Proteomic and metabolomic integration reveals the effects of pre-flowering cytokinin applications on central carbon metabolism in table grape berries
Consumers around the world prefer high quality table grapes. To achieve higher quality traits at ripening, grapevine producers apply different plant growth regulators. The synthetic cytokinin forchlorfenuron N-(2-chloro-4-pyridinyl)-N'-phenylurea (CPPU) is widely used, its effect on grape quality is...
| Autores principales: | , , , , , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2023
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/128483 |
| _version_ | 1855535518765285376 |
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| author | Olmedo, Patricio Núñez-Lillo, Gerardo Vidal, Juan Leiva, Carol Rojas, Bárbara Sagredo, Karen Arriagada, César Defilippi, Bruno G. Pérez Donoso, Alonso G. Meneses, Claudio Carpentier, Sebastien C. Pedreschi, Romina Campos Vargas, Reinaldo |
| author_browse | Arriagada, César Campos Vargas, Reinaldo Carpentier, Sebastien C. Defilippi, Bruno G. Leiva, Carol Meneses, Claudio Núñez-Lillo, Gerardo Olmedo, Patricio Pedreschi, Romina Pérez Donoso, Alonso G. Rojas, Bárbara Sagredo, Karen Vidal, Juan |
| author_facet | Olmedo, Patricio Núñez-Lillo, Gerardo Vidal, Juan Leiva, Carol Rojas, Bárbara Sagredo, Karen Arriagada, César Defilippi, Bruno G. Pérez Donoso, Alonso G. Meneses, Claudio Carpentier, Sebastien C. Pedreschi, Romina Campos Vargas, Reinaldo |
| author_sort | Olmedo, Patricio |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Consumers around the world prefer high quality table grapes. To achieve higher quality traits at ripening, grapevine producers apply different plant growth regulators. The synthetic cytokinin forchlorfenuron N-(2-chloro-4-pyridinyl)-N'-phenylurea (CPPU) is widely used, its effect on grape quality is poorly understood. We hypothesized that the use of CPPU in pre-flowering can lead to changes in the metabolism that affects grape quality at harvest. Therefore, we investigated the role of CPPU applications on the quality of grapes by integrating proteomics and metabolomics. CPPU-treated grapevines showed a significant increase in berry size and firmness. Proteomic analyses indicated that CPPU-treated berries accumulated enzymes associated with carbohydrate metabolism, glycolysis, and tricarboxylic acid (TCA) cycle at harvest. Metabolomic analyses showed shifts in the abundance of compounds associated with carbohydrate metabolism and TCA cycle in CPPU-treated grapes. These findings suggest that CPPU applications modulate central carbon metabolism, improving grape berry quality. |
| format | Journal Article |
| id | CGSpace128483 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | CGSpace1284832025-10-26T13:02:31Z Proteomic and metabolomic integration reveals the effects of pre-flowering cytokinin applications on central carbon metabolism in table grape berries Olmedo, Patricio Núñez-Lillo, Gerardo Vidal, Juan Leiva, Carol Rojas, Bárbara Sagredo, Karen Arriagada, César Defilippi, Bruno G. Pérez Donoso, Alonso G. Meneses, Claudio Carpentier, Sebastien C. Pedreschi, Romina Campos Vargas, Reinaldo vitis vinifera metabolomics proteomics glycolysis tca metabolismo proteómica glicólisis Consumers around the world prefer high quality table grapes. To achieve higher quality traits at ripening, grapevine producers apply different plant growth regulators. The synthetic cytokinin forchlorfenuron N-(2-chloro-4-pyridinyl)-N'-phenylurea (CPPU) is widely used, its effect on grape quality is poorly understood. We hypothesized that the use of CPPU in pre-flowering can lead to changes in the metabolism that affects grape quality at harvest. Therefore, we investigated the role of CPPU applications on the quality of grapes by integrating proteomics and metabolomics. CPPU-treated grapevines showed a significant increase in berry size and firmness. Proteomic analyses indicated that CPPU-treated berries accumulated enzymes associated with carbohydrate metabolism, glycolysis, and tricarboxylic acid (TCA) cycle at harvest. Metabolomic analyses showed shifts in the abundance of compounds associated with carbohydrate metabolism and TCA cycle in CPPU-treated grapes. These findings suggest that CPPU applications modulate central carbon metabolism, improving grape berry quality. 2023-06 2023-02-07T11:55:24Z 2023-02-07T11:55:24Z Journal Article https://hdl.handle.net/10568/128483 en Limited Access Elsevier Olmedo, P.; Núñez-Lillo, G.; Vidal, J.; Leiva, C.; Rojas, B.; Sagredo, K.; Arriagada, C.; Defilippi, B.G.; Pérez-Donoso, A.G.; Meneses, C.; Carpentier, S.; Pedreschi, R.; Campos-Vargas, R. (2023) Proteomic and metabolomic integration reveals the effects of pre-flowering cytokinin applications on central carbon metabolism in table grape berries. Food Chemistry 411: 135498. ISSN: 0308-8146 |
| spellingShingle | vitis vinifera metabolomics proteomics glycolysis tca metabolismo proteómica glicólisis Olmedo, Patricio Núñez-Lillo, Gerardo Vidal, Juan Leiva, Carol Rojas, Bárbara Sagredo, Karen Arriagada, César Defilippi, Bruno G. Pérez Donoso, Alonso G. Meneses, Claudio Carpentier, Sebastien C. Pedreschi, Romina Campos Vargas, Reinaldo Proteomic and metabolomic integration reveals the effects of pre-flowering cytokinin applications on central carbon metabolism in table grape berries |
| title | Proteomic and metabolomic integration reveals the effects of pre-flowering cytokinin applications on central carbon metabolism in table grape berries |
| title_full | Proteomic and metabolomic integration reveals the effects of pre-flowering cytokinin applications on central carbon metabolism in table grape berries |
| title_fullStr | Proteomic and metabolomic integration reveals the effects of pre-flowering cytokinin applications on central carbon metabolism in table grape berries |
| title_full_unstemmed | Proteomic and metabolomic integration reveals the effects of pre-flowering cytokinin applications on central carbon metabolism in table grape berries |
| title_short | Proteomic and metabolomic integration reveals the effects of pre-flowering cytokinin applications on central carbon metabolism in table grape berries |
| title_sort | proteomic and metabolomic integration reveals the effects of pre flowering cytokinin applications on central carbon metabolism in table grape berries |
| topic | vitis vinifera metabolomics proteomics glycolysis tca metabolismo proteómica glicólisis |
| url | https://hdl.handle.net/10568/128483 |
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