Loop-mediated Isothermal Amplification (LAMP): Potato virus detection CIP – SOPXXX, v.1.1.
This standard operational procedure (SOP) for LAMP testing covers the steps on how to use a field-deployable LAMP assay in the diagnosis of the major viruses infecting potato worldwide: Potato leaf roll virus (PLRV), Potato virus Y (PVY), and Potato virus X (PVX). It includes different steps from co...
| Autor principal: | |
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| Formato: | Manual |
| Lenguaje: | Inglés |
| Publicado: |
International Potato Center
2022
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| Acceso en línea: | https://hdl.handle.net/10568/128477 |
| _version_ | 1855531265283850240 |
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| author | Fuentes, S. |
| author_browse | Fuentes, S. |
| author_facet | Fuentes, S. |
| author_sort | Fuentes, S. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | This standard operational procedure (SOP) for LAMP testing covers the steps on how to use a field-deployable LAMP assay in the diagnosis of the major viruses infecting potato worldwide: Potato leaf roll virus (PLRV), Potato virus Y (PVY), and Potato virus X (PVX). It includes different steps from collecting samples, processing of them for nucleic acid extraction, preparing LAMP reaction, setting the Genie instrument, and recording of results. LAMP test can be rapidly adopted to detect different pathogens in different plants by just replacing the specific primers (for those pathogens) in the reaction, which makes it broadly applicable and adjustable to new pathogens. |
| format | Manual |
| id | CGSpace128477 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | International Potato Center |
| publisherStr | International Potato Center |
| record_format | dspace |
| spelling | CGSpace1284772025-11-06T14:08:26Z Loop-mediated Isothermal Amplification (LAMP): Potato virus detection CIP – SOPXXX, v.1.1. Fuentes, S. potatoes viroses plant viruses detection diagnosis This standard operational procedure (SOP) for LAMP testing covers the steps on how to use a field-deployable LAMP assay in the diagnosis of the major viruses infecting potato worldwide: Potato leaf roll virus (PLRV), Potato virus Y (PVY), and Potato virus X (PVX). It includes different steps from collecting samples, processing of them for nucleic acid extraction, preparing LAMP reaction, setting the Genie instrument, and recording of results. LAMP test can be rapidly adopted to detect different pathogens in different plants by just replacing the specific primers (for those pathogens) in the reaction, which makes it broadly applicable and adjustable to new pathogens. 2022-10 2023-02-06T19:59:12Z 2023-02-06T19:59:12Z Manual https://hdl.handle.net/10568/128477 en Open Access application/pdf International Potato Center Fuentes, S. 2022. Loop-mediated Isothermal Amplification (LAMP): Potato virus detection CIP – SOPXXX, v.1.1. International Potato Center. ISBN: 978-92-9060-603-1. 14 p. |
| spellingShingle | potatoes viroses plant viruses detection diagnosis Fuentes, S. Loop-mediated Isothermal Amplification (LAMP): Potato virus detection CIP – SOPXXX, v.1.1. |
| title | Loop-mediated Isothermal Amplification (LAMP): Potato virus detection CIP – SOPXXX, v.1.1. |
| title_full | Loop-mediated Isothermal Amplification (LAMP): Potato virus detection CIP – SOPXXX, v.1.1. |
| title_fullStr | Loop-mediated Isothermal Amplification (LAMP): Potato virus detection CIP – SOPXXX, v.1.1. |
| title_full_unstemmed | Loop-mediated Isothermal Amplification (LAMP): Potato virus detection CIP – SOPXXX, v.1.1. |
| title_short | Loop-mediated Isothermal Amplification (LAMP): Potato virus detection CIP – SOPXXX, v.1.1. |
| title_sort | loop mediated isothermal amplification lamp potato virus detection cip sopxxx v 1 1 |
| topic | potatoes viroses plant viruses detection diagnosis |
| url | https://hdl.handle.net/10568/128477 |
| work_keys_str_mv | AT fuentess loopmediatedisothermalamplificationlamppotatovirusdetectioncipsopxxxv11 |