Genome-wide association for heat tolerance at seedling stage in historical spring wheat cultivars
Increasing global temperature has adverse effects on crop health and productivity at both seedling and reproductivity stages. It is paramount to develop heat tolerant wheat cultivars able to sustain under high and fluctuating temperature conditions. An experiment was conducted to characterize 194 hi...
| Autores principales: | , , , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Frontiers Media
2022
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/128239 |
| _version_ | 1855534525886496768 |
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| author | Khan, Muhammad Ibrar Kainat, Zarnishal Maqbool, Saman Mehwish, Ambreen Ahmad, Suhaib Hafiz Muhammad Suleman Mahmood, Zahid Ali, Mohsin Aziz, Abdul Rasheed, Awais Huihui Li |
| author_browse | Ahmad, Suhaib Ali, Mohsin Aziz, Abdul Hafiz Muhammad Suleman Huihui Li Kainat, Zarnishal Khan, Muhammad Ibrar Mahmood, Zahid Maqbool, Saman Mehwish, Ambreen Rasheed, Awais |
| author_facet | Khan, Muhammad Ibrar Kainat, Zarnishal Maqbool, Saman Mehwish, Ambreen Ahmad, Suhaib Hafiz Muhammad Suleman Mahmood, Zahid Ali, Mohsin Aziz, Abdul Rasheed, Awais Huihui Li |
| author_sort | Khan, Muhammad Ibrar |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Increasing global temperature has adverse effects on crop health and productivity at both seedling and reproductivity stages. It is paramount to develop heat tolerant wheat cultivars able to sustain under high and fluctuating temperature conditions. An experiment was conducted to characterize 194 historical wheat cultivars of Pakistan under high temperature at seedling stage to identify loci associated with heat tolerance using genome-wide association studies (GWAS). A quantitative trait locus, TaHST1, on chr4A was also characterized to identify the haplotypes at this locus associated with heat tolerance in wheat from Pakistan. Initially, the diversity panel was planted under control conditions (25°C/20°C day and night temperature) in a glass house. At three leaf stage, plants were subjected to heat stress (HS) by increasing temperature (40°C/35°C day and night), while one treatment was kept at control condition. After 7 days of HS, data were collected for seedling morphology. Heat stress reduced these traits by 25% (root weight) to 40% (shoot weight), and shoot biomass was largely affected by heat stress. A GWAS model, fixed and random model circulating probability unification (FarmCPU), identified 43 quantitative trait nucleotides (QTNs) on all chromosomes, except chr7B, were associated under both HS and control conditions. Thirteen QTNs were identified in control, while 30 QTNs were identified in HS condition. In total, 24 haplotypes were identified at TaHST1 locus, and most of the heat tolerant genotypes were assigned to Hap-20 and Hap-21. Eleven QTNs were identified within 0.3–3.1 Mb proximity of heat shock protein (HSP). Conclusively, this study provided a detailed genetic framework of heat tolerance in wheat at the seedling stage and identify potential genetic regions associated with heat tolerance which can be used for marker assisted selection (MAS) in breeding for heat stress tolerance. |
| format | Journal Article |
| id | CGSpace128239 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Frontiers Media |
| publisherStr | Frontiers Media |
| record_format | dspace |
| spelling | CGSpace1282392025-12-08T10:29:22Z Genome-wide association for heat tolerance at seedling stage in historical spring wheat cultivars Khan, Muhammad Ibrar Kainat, Zarnishal Maqbool, Saman Mehwish, Ambreen Ahmad, Suhaib Hafiz Muhammad Suleman Mahmood, Zahid Ali, Mohsin Aziz, Abdul Rasheed, Awais Huihui Li heat stress quantitative trait loci nucleotides single nucleotide polymorphism spring wheat Increasing global temperature has adverse effects on crop health and productivity at both seedling and reproductivity stages. It is paramount to develop heat tolerant wheat cultivars able to sustain under high and fluctuating temperature conditions. An experiment was conducted to characterize 194 historical wheat cultivars of Pakistan under high temperature at seedling stage to identify loci associated with heat tolerance using genome-wide association studies (GWAS). A quantitative trait locus, TaHST1, on chr4A was also characterized to identify the haplotypes at this locus associated with heat tolerance in wheat from Pakistan. Initially, the diversity panel was planted under control conditions (25°C/20°C day and night temperature) in a glass house. At three leaf stage, plants were subjected to heat stress (HS) by increasing temperature (40°C/35°C day and night), while one treatment was kept at control condition. After 7 days of HS, data were collected for seedling morphology. Heat stress reduced these traits by 25% (root weight) to 40% (shoot weight), and shoot biomass was largely affected by heat stress. A GWAS model, fixed and random model circulating probability unification (FarmCPU), identified 43 quantitative trait nucleotides (QTNs) on all chromosomes, except chr7B, were associated under both HS and control conditions. Thirteen QTNs were identified in control, while 30 QTNs were identified in HS condition. In total, 24 haplotypes were identified at TaHST1 locus, and most of the heat tolerant genotypes were assigned to Hap-20 and Hap-21. Eleven QTNs were identified within 0.3–3.1 Mb proximity of heat shock protein (HSP). Conclusively, this study provided a detailed genetic framework of heat tolerance in wheat at the seedling stage and identify potential genetic regions associated with heat tolerance which can be used for marker assisted selection (MAS) in breeding for heat stress tolerance. 2022-08-25 2023-01-25T20:02:44Z 2023-01-25T20:02:44Z Journal Article https://hdl.handle.net/10568/128239 en Open Access application/pdf Frontiers Media Khan, M. I., Kainat, Z., Maqbool, S., Mehwish, A., Ahmad, S., Suleman, H. M., Mahmood, Z., Ali, M., Aziz, A., Rasheed, A., & Li, H. (2022). Genome-wide association for heat tolerance at seedling stage in historical spring wheat cultivars. Frontiers in Plant Science, 13. https://doi.org/10.3389/fpls.2022.972481 |
| spellingShingle | heat stress quantitative trait loci nucleotides single nucleotide polymorphism spring wheat Khan, Muhammad Ibrar Kainat, Zarnishal Maqbool, Saman Mehwish, Ambreen Ahmad, Suhaib Hafiz Muhammad Suleman Mahmood, Zahid Ali, Mohsin Aziz, Abdul Rasheed, Awais Huihui Li Genome-wide association for heat tolerance at seedling stage in historical spring wheat cultivars |
| title | Genome-wide association for heat tolerance at seedling stage in historical spring wheat cultivars |
| title_full | Genome-wide association for heat tolerance at seedling stage in historical spring wheat cultivars |
| title_fullStr | Genome-wide association for heat tolerance at seedling stage in historical spring wheat cultivars |
| title_full_unstemmed | Genome-wide association for heat tolerance at seedling stage in historical spring wheat cultivars |
| title_short | Genome-wide association for heat tolerance at seedling stage in historical spring wheat cultivars |
| title_sort | genome wide association for heat tolerance at seedling stage in historical spring wheat cultivars |
| topic | heat stress quantitative trait loci nucleotides single nucleotide polymorphism spring wheat |
| url | https://hdl.handle.net/10568/128239 |
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