Assessment of Pathogenicity Assay of Ascochyta rabiei Isolates Using Chickpea Differential Genotypes
Ascochyta blight caused by Ascochyta rabiei, is the most destructive disease in many chickpea growing countries. Many chickpea varieties were out of production due to increased virulence/aggressiveness of the pathogen in some countries. The aim of this study was to assess variation in pathogenicity...
| Main Authors: | , , , , |
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| Format: | Conference Paper |
| Language: | Inglés |
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Australian National Chickpea Ascochyta Blight
2022
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| Online Access: | https://hdl.handle.net/10568/128125 |
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| author | Istanbuli, Tawffiq Tawkaz, Sawsan Moukahel, Abdulrahman Kemal, Seid Ahmed Hamwieh, Aladdin |
| author_browse | Hamwieh, Aladdin Istanbuli, Tawffiq Kemal, Seid Ahmed Moukahel, Abdulrahman Tawkaz, Sawsan |
| author_facet | Istanbuli, Tawffiq Tawkaz, Sawsan Moukahel, Abdulrahman Kemal, Seid Ahmed Hamwieh, Aladdin |
| author_sort | Istanbuli, Tawffiq |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Ascochyta blight caused by Ascochyta rabiei, is the most destructive disease in many chickpea growing countries. Many chickpea varieties were out of production due to increased virulence/aggressiveness of the pathogen in some countries. The aim of this study was to assess variation in pathogenicity of 25 A. rabiei collected in different years from Syria, Lebanon, Uzbekistan, and France. The pathogen phenotyping was done using five genotypes (ILC3279, ILC482, ICC3396, ICC12004, and Genesis090) under controlled conditions (humidity >85, temperature 18-20 °C, light/dark 18:6 h) at the International Center of Agricultural Research in the Dry Area (ICARDA), Terbol station, Lebanon. Five genotypes carrying different resistant genes have been used in this study, and a highly susceptible genotype (ILC263). was used as a control. A total of 25 sets of these genotypes, each genotype has two replications that have been planted and inoculated with the 25 A. rabiei isolates. The symptoms were measured two times using a 1–9 disease symptoms rating scale (RS) when the susceptible control was RS >6. The result showed high significant differences between genotypes (G) (P<0.001), and isolations (P<0.001). The average of resistance for the five genotypes was 3.5 compared to the susceptible check which scored ≥6. The results indicated 5 isolates were highly aggressive isolates Pathotype 4 & race 4 from Syria, Uz3-2 from Uzbekistan, and FR-2926 & FR-3248 from France). Further large-scale evaluation of these isolates on the AB differential set may help to identify a better understanding of the resistant genes available in chickpeas. |
| format | Conference Paper |
| id | CGSpace128125 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Australian National Chickpea Ascochyta Blight |
| publisherStr | Australian National Chickpea Ascochyta Blight |
| record_format | dspace |
| spelling | CGSpace1281252026-01-21T02:10:34Z Assessment of Pathogenicity Assay of Ascochyta rabiei Isolates Using Chickpea Differential Genotypes Istanbuli, Tawffiq Tawkaz, Sawsan Moukahel, Abdulrahman Kemal, Seid Ahmed Hamwieh, Aladdin food security health nutrition chickpeas chickpea ascochyta blight climate adaptation and mitigation Ascochyta blight caused by Ascochyta rabiei, is the most destructive disease in many chickpea growing countries. Many chickpea varieties were out of production due to increased virulence/aggressiveness of the pathogen in some countries. The aim of this study was to assess variation in pathogenicity of 25 A. rabiei collected in different years from Syria, Lebanon, Uzbekistan, and France. The pathogen phenotyping was done using five genotypes (ILC3279, ILC482, ICC3396, ICC12004, and Genesis090) under controlled conditions (humidity >85, temperature 18-20 °C, light/dark 18:6 h) at the International Center of Agricultural Research in the Dry Area (ICARDA), Terbol station, Lebanon. Five genotypes carrying different resistant genes have been used in this study, and a highly susceptible genotype (ILC263). was used as a control. A total of 25 sets of these genotypes, each genotype has two replications that have been planted and inoculated with the 25 A. rabiei isolates. The symptoms were measured two times using a 1–9 disease symptoms rating scale (RS) when the susceptible control was RS >6. The result showed high significant differences between genotypes (G) (P<0.001), and isolations (P<0.001). The average of resistance for the five genotypes was 3.5 compared to the susceptible check which scored ≥6. The results indicated 5 isolates were highly aggressive isolates Pathotype 4 & race 4 from Syria, Uz3-2 from Uzbekistan, and FR-2926 & FR-3248 from France). Further large-scale evaluation of these isolates on the AB differential set may help to identify a better understanding of the resistant genes available in chickpeas. 2022-10-12 2023-01-24T21:40:09Z 2023-01-24T21:40:09Z Conference Paper https://hdl.handle.net/10568/128125 en Limited Access application/pdf Australian National Chickpea Ascochyta Blight Tawffiq Istanbuli, Sawsan Tawkaz, Abdulrahman Moukahel, Seid Ahmed Kemal, Aladdin Hamwieh. (12/10/2022). Assessment of Pathogenicity Assay of Ascochyta rabiei Isolates Using Chickpea Differential Genotypes. Wagga Wagga, Australia. |
| spellingShingle | food security health nutrition chickpeas chickpea ascochyta blight climate adaptation and mitigation Istanbuli, Tawffiq Tawkaz, Sawsan Moukahel, Abdulrahman Kemal, Seid Ahmed Hamwieh, Aladdin Assessment of Pathogenicity Assay of Ascochyta rabiei Isolates Using Chickpea Differential Genotypes |
| title | Assessment of Pathogenicity Assay of Ascochyta rabiei Isolates Using Chickpea Differential Genotypes |
| title_full | Assessment of Pathogenicity Assay of Ascochyta rabiei Isolates Using Chickpea Differential Genotypes |
| title_fullStr | Assessment of Pathogenicity Assay of Ascochyta rabiei Isolates Using Chickpea Differential Genotypes |
| title_full_unstemmed | Assessment of Pathogenicity Assay of Ascochyta rabiei Isolates Using Chickpea Differential Genotypes |
| title_short | Assessment of Pathogenicity Assay of Ascochyta rabiei Isolates Using Chickpea Differential Genotypes |
| title_sort | assessment of pathogenicity assay of ascochyta rabiei isolates using chickpea differential genotypes |
| topic | food security health nutrition chickpeas chickpea ascochyta blight climate adaptation and mitigation |
| url | https://hdl.handle.net/10568/128125 |
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