Genetic diversity of two viruses affecting lentil and chickpea crops in the highlands of Ethiopia

Chickpea and lentil are the major legume crops grown in Ethiopia, providing people with balanced nutrition of proteins, minerals, fibers, and carbohydrates. Since 2019, farmers in the highlands of Ethiopia are losing their crops because of virus epidemics, and lentil production area in Ethiopia was...

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Main Authors: Kumari, Safaa, Moukahel, Abdulrahman, Ademe, Anteneh, Aynewa, Yetsedaw, Kemal, Seid Ahmed
Format: Internal Document
Language:Inglés
Published: International Center for Agricultural Research in the Dry Areas 2022
Subjects:
Online Access:https://hdl.handle.net/10568/127946
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author Kumari, Safaa
Moukahel, Abdulrahman
Ademe, Anteneh
Aynewa, Yetsedaw
Kemal, Seid Ahmed
author_browse Ademe, Anteneh
Aynewa, Yetsedaw
Kemal, Seid Ahmed
Kumari, Safaa
Moukahel, Abdulrahman
author_facet Kumari, Safaa
Moukahel, Abdulrahman
Ademe, Anteneh
Aynewa, Yetsedaw
Kemal, Seid Ahmed
author_sort Kumari, Safaa
collection Repository of Agricultural Research Outputs (CGSpace)
description Chickpea and lentil are the major legume crops grown in Ethiopia, providing people with balanced nutrition of proteins, minerals, fibers, and carbohydrates. Since 2019, farmers in the highlands of Ethiopia are losing their crops because of virus epidemics, and lentil production area in Ethiopia was reduced due to the virus infection. Knowing genetic variation and the exact identity of viruses affecting crops is essential for breeding for resistance and crop management purposes. Serological tests for more than 2000 chickpea and lentil samples showed that the most important viruses are Chickpea chlorotic stunt virus (CpCSV) on both crops, and Pea seed-borne mosaic virus (PSbMV) on lentil. Representative samples were selected for further analysis with RT-PCR and sequence to identify the isolates at molecular level. Phylogenetic analysis showed that the Ethiopian PSbMV isolates (5 lentil & 1 chickpea) are clustered with pathotype 2, and CpCSV isolates (3 chickpea & 2 lentil) are clustered with Serogroup-I.
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publisher International Center for Agricultural Research in the Dry Areas
publisherStr International Center for Agricultural Research in the Dry Areas
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spelling CGSpace1279462026-01-15T02:00:24Z Genetic diversity of two viruses affecting lentil and chickpea crops in the highlands of Ethiopia Kumari, Safaa Moukahel, Abdulrahman Ademe, Anteneh Aynewa, Yetsedaw Kemal, Seid Ahmed food security molecular characterization virus diseases lentil chickpea Chickpea and lentil are the major legume crops grown in Ethiopia, providing people with balanced nutrition of proteins, minerals, fibers, and carbohydrates. Since 2019, farmers in the highlands of Ethiopia are losing their crops because of virus epidemics, and lentil production area in Ethiopia was reduced due to the virus infection. Knowing genetic variation and the exact identity of viruses affecting crops is essential for breeding for resistance and crop management purposes. Serological tests for more than 2000 chickpea and lentil samples showed that the most important viruses are Chickpea chlorotic stunt virus (CpCSV) on both crops, and Pea seed-borne mosaic virus (PSbMV) on lentil. Representative samples were selected for further analysis with RT-PCR and sequence to identify the isolates at molecular level. Phylogenetic analysis showed that the Ethiopian PSbMV isolates (5 lentil & 1 chickpea) are clustered with pathotype 2, and CpCSV isolates (3 chickpea & 2 lentil) are clustered with Serogroup-I. 2022-12-01 2023-01-24T03:06:33Z 2023-01-24T03:06:33Z Internal Document https://hdl.handle.net/10568/127946 en Open Access application/pdf International Center for Agricultural Research in the Dry Areas Safaa G. Kumari, Abdulrahman Moukahel, Anteneh Ademe, Yetsedaw Aynewa and Seid Ahmed Kemal. 2022. Genetic diversity of two viruses affecting lentil and chickpea crops in the highlands of Ethiopia. International Center for Agricultural Research in the Dry Areas (ICARDA), Beirut, Lebanon.
spellingShingle food security
molecular characterization
virus diseases
lentil
chickpea
Kumari, Safaa
Moukahel, Abdulrahman
Ademe, Anteneh
Aynewa, Yetsedaw
Kemal, Seid Ahmed
Genetic diversity of two viruses affecting lentil and chickpea crops in the highlands of Ethiopia
title Genetic diversity of two viruses affecting lentil and chickpea crops in the highlands of Ethiopia
title_full Genetic diversity of two viruses affecting lentil and chickpea crops in the highlands of Ethiopia
title_fullStr Genetic diversity of two viruses affecting lentil and chickpea crops in the highlands of Ethiopia
title_full_unstemmed Genetic diversity of two viruses affecting lentil and chickpea crops in the highlands of Ethiopia
title_short Genetic diversity of two viruses affecting lentil and chickpea crops in the highlands of Ethiopia
title_sort genetic diversity of two viruses affecting lentil and chickpea crops in the highlands of ethiopia
topic food security
molecular characterization
virus diseases
lentil
chickpea
url https://hdl.handle.net/10568/127946
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