Reverse transcription-polymerase chain reaction (RT-PCR) assay for detection and characterization of Pea seed-borne mosaic virus in legumes applied by ICARDA’s GHU
Pea seed-borne mosaic virus (PSbMV, genus Potyvirus, family Potyviridae) is the most important virus infecting lentil both in terms of economic importance and geographical distribution. It was reported that PSbMV can even lead up to 61% yield loss. Therefore, developing and implementing effective ma...
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| Format: | Tool |
| Language: | Inglés |
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International Center for Agricultural Research in the Dry Areas
2022
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| Online Access: | https://hdl.handle.net/10568/127945 |
| _version_ | 1855527062408790016 |
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| author | Kumari, Safaa Moukahel, Abdulrahman |
| author_browse | Kumari, Safaa Moukahel, Abdulrahman |
| author_facet | Kumari, Safaa Moukahel, Abdulrahman |
| author_sort | Kumari, Safaa |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Pea seed-borne mosaic virus (PSbMV, genus Potyvirus, family Potyviridae) is the most important virus infecting lentil both in terms of economic importance and geographical distribution. It was reported that PSbMV can even lead up to 61% yield loss. Therefore, developing and implementing effective management strategies for PSbMV is very important to reduce yield and quality loss. However, accurate detection of virus disease is the first essential step for effective management strategies for control of this disease. Molecular is efficient tool to accurately detect and monitor the occurrence and development of crop diseases. This report describes a validated reverse transcription-polymerase chain reaction (RT-PCR) assay for PSbMV detection and characterization applied by ICARDA’s GHU. |
| format | Tool |
| id | CGSpace127945 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | International Center for Agricultural Research in the Dry Areas |
| publisherStr | International Center for Agricultural Research in the Dry Areas |
| record_format | dspace |
| spelling | CGSpace1279452026-01-15T02:09:56Z Reverse transcription-polymerase chain reaction (RT-PCR) assay for detection and characterization of Pea seed-borne mosaic virus in legumes applied by ICARDA’s GHU Kumari, Safaa Moukahel, Abdulrahman food security molecular characterization virus diseases plant health faba bean lentil chickpea grass pea (lathyrus sativus) seed health testing seed-borne virus field pea (pisum sativum) Pea seed-borne mosaic virus (PSbMV, genus Potyvirus, family Potyviridae) is the most important virus infecting lentil both in terms of economic importance and geographical distribution. It was reported that PSbMV can even lead up to 61% yield loss. Therefore, developing and implementing effective management strategies for PSbMV is very important to reduce yield and quality loss. However, accurate detection of virus disease is the first essential step for effective management strategies for control of this disease. Molecular is efficient tool to accurately detect and monitor the occurrence and development of crop diseases. This report describes a validated reverse transcription-polymerase chain reaction (RT-PCR) assay for PSbMV detection and characterization applied by ICARDA’s GHU. 2022-12-01 2023-01-24T03:04:45Z 2023-01-24T03:04:45Z Tool https://hdl.handle.net/10568/127945 en Open Access application/pdf International Center for Agricultural Research in the Dry Areas Safaa G. Kumari and Abdelrahman Moukahel. 2022. Reverse transcription-polymerase chain reaction (RT-PCR) assay for detection and characterization of Pea seed-borne mosaic virus in legumes applied by ICARDA’s GHU. International Center for Agricultural Research in the Dry Areas (ICARDA), Beirut, Lebanon. |
| spellingShingle | food security molecular characterization virus diseases plant health faba bean lentil chickpea grass pea (lathyrus sativus) seed health testing seed-borne virus field pea (pisum sativum) Kumari, Safaa Moukahel, Abdulrahman Reverse transcription-polymerase chain reaction (RT-PCR) assay for detection and characterization of Pea seed-borne mosaic virus in legumes applied by ICARDA’s GHU |
| title | Reverse transcription-polymerase chain reaction (RT-PCR) assay for detection and characterization of Pea seed-borne mosaic virus in legumes applied by ICARDA’s GHU |
| title_full | Reverse transcription-polymerase chain reaction (RT-PCR) assay for detection and characterization of Pea seed-borne mosaic virus in legumes applied by ICARDA’s GHU |
| title_fullStr | Reverse transcription-polymerase chain reaction (RT-PCR) assay for detection and characterization of Pea seed-borne mosaic virus in legumes applied by ICARDA’s GHU |
| title_full_unstemmed | Reverse transcription-polymerase chain reaction (RT-PCR) assay for detection and characterization of Pea seed-borne mosaic virus in legumes applied by ICARDA’s GHU |
| title_short | Reverse transcription-polymerase chain reaction (RT-PCR) assay for detection and characterization of Pea seed-borne mosaic virus in legumes applied by ICARDA’s GHU |
| title_sort | reverse transcription polymerase chain reaction rt pcr assay for detection and characterization of pea seed borne mosaic virus in legumes applied by icarda s ghu |
| topic | food security molecular characterization virus diseases plant health faba bean lentil chickpea grass pea (lathyrus sativus) seed health testing seed-borne virus field pea (pisum sativum) |
| url | https://hdl.handle.net/10568/127945 |
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