Large genomic introgression blocks of Phaseolus parvifolius Freytag bean into the common bean enhance the crossability between tepary and common beans

The production of the common bean (Phaseolus vulgaris L.), one of the most important sources of protein and minerals and one of the most consumed grain legumes globally, is highly affected by heat and drought constraints. In contrast, the tepary bean (Phaseolus acutifolius A. Gray), a common bean-re...

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Main Authors: Barrera, Santos, Berny Mier y Teran, Jorge Carlos, Lobaton, Juan David, Escobar Pérez, Roosevelt Humberto, Gepts, Paul L., Beebe, Stephen E., Urrea, Carlos A.
Format: Journal Article
Language:Inglés
Published: Wiley 2022
Subjects:
Online Access:https://hdl.handle.net/10568/127311
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author Barrera, Santos
Berny Mier y Teran, Jorge Carlos
Lobaton, Juan David
Escobar Pérez, Roosevelt Humberto
Gepts, Paul L.
Beebe, Stephen E.
Urrea, Carlos A.
author_browse Barrera, Santos
Beebe, Stephen E.
Berny Mier y Teran, Jorge Carlos
Escobar Pérez, Roosevelt Humberto
Gepts, Paul L.
Lobaton, Juan David
Urrea, Carlos A.
author_facet Barrera, Santos
Berny Mier y Teran, Jorge Carlos
Lobaton, Juan David
Escobar Pérez, Roosevelt Humberto
Gepts, Paul L.
Beebe, Stephen E.
Urrea, Carlos A.
author_sort Barrera, Santos
collection Repository of Agricultural Research Outputs (CGSpace)
description The production of the common bean (Phaseolus vulgaris L.), one of the most important sources of protein and minerals and one of the most consumed grain legumes globally, is highly affected by heat and drought constraints. In contrast, the tepary bean (Phaseolus acutifolius A. Gray), a common bean-related species, is adapted to hot and dry climates. Hybridization to introduce complex traits from the tepary bean into the common bean has been challenging, as embryo rescue is required. In this study, we report three novel interspecific lines that were obtained by crossing lines from prior common bean tepary bean hybridization with Phaseolus parvifolius Frey tag in order to increase the male gametic diversity to facilitate interspecific crosses. These interspecific lines enhanced the crossability of the common bean and tepary bean species while avoiding the embryo rescue process. Crossing these three interspecific lines with tepary beans resulted in 12-fold more hybrid plants than crossing traditional common beans with tepary beans. Whole-genome sequencing analysis of these three interspecific lines shows large introgressions of genomic regions corresponding to P. parvifolius on chromosomes that presumably contribute to reproductive barriers between both species. The development of these lines opens up the possibility of increasing the introgression of desirable tepary bean traits into the common bean to address constraints driven by climate change.
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spelling CGSpace1273112025-11-11T17:40:15Z Large genomic introgression blocks of Phaseolus parvifolius Freytag bean into the common bean enhance the crossability between tepary and common beans Barrera, Santos Berny Mier y Teran, Jorge Carlos Lobaton, Juan David Escobar Pérez, Roosevelt Humberto Gepts, Paul L. Beebe, Stephen E. Urrea, Carlos A. embryo preservation haplotypes gene editing conservación de embriones haplotipos edición de genes ecology The production of the common bean (Phaseolus vulgaris L.), one of the most important sources of protein and minerals and one of the most consumed grain legumes globally, is highly affected by heat and drought constraints. In contrast, the tepary bean (Phaseolus acutifolius A. Gray), a common bean-related species, is adapted to hot and dry climates. Hybridization to introduce complex traits from the tepary bean into the common bean has been challenging, as embryo rescue is required. In this study, we report three novel interspecific lines that were obtained by crossing lines from prior common bean tepary bean hybridization with Phaseolus parvifolius Frey tag in order to increase the male gametic diversity to facilitate interspecific crosses. These interspecific lines enhanced the crossability of the common bean and tepary bean species while avoiding the embryo rescue process. Crossing these three interspecific lines with tepary beans resulted in 12-fold more hybrid plants than crossing traditional common beans with tepary beans. Whole-genome sequencing analysis of these three interspecific lines shows large introgressions of genomic regions corresponding to P. parvifolius on chromosomes that presumably contribute to reproductive barriers between both species. The development of these lines opens up the possibility of increasing the introgression of desirable tepary bean traits into the common bean to address constraints driven by climate change. 2022-12 2023-01-17T15:13:47Z 2023-01-17T15:13:47Z Journal Article https://hdl.handle.net/10568/127311 en Open Access application/pdf Wiley Barrera, S.; Berny Mier Y.Teran, J.C.; Lobaton, J.D.; Escobar, R.; Gepts, P.; Beebe, S.; Urrea, C.A. (2022) Large genomic introgression blocks of Phaseolus parvifolius Freytag bean into the common bean enhance the crossability between tepary and common beans. Plant Direct 6(12):e470 12 p. ISSN: 2475-4455
spellingShingle embryo preservation
haplotypes
gene editing
conservación de embriones
haplotipos
edición de genes
ecology
Barrera, Santos
Berny Mier y Teran, Jorge Carlos
Lobaton, Juan David
Escobar Pérez, Roosevelt Humberto
Gepts, Paul L.
Beebe, Stephen E.
Urrea, Carlos A.
Large genomic introgression blocks of Phaseolus parvifolius Freytag bean into the common bean enhance the crossability between tepary and common beans
title Large genomic introgression blocks of Phaseolus parvifolius Freytag bean into the common bean enhance the crossability between tepary and common beans
title_full Large genomic introgression blocks of Phaseolus parvifolius Freytag bean into the common bean enhance the crossability between tepary and common beans
title_fullStr Large genomic introgression blocks of Phaseolus parvifolius Freytag bean into the common bean enhance the crossability between tepary and common beans
title_full_unstemmed Large genomic introgression blocks of Phaseolus parvifolius Freytag bean into the common bean enhance the crossability between tepary and common beans
title_short Large genomic introgression blocks of Phaseolus parvifolius Freytag bean into the common bean enhance the crossability between tepary and common beans
title_sort large genomic introgression blocks of phaseolus parvifolius freytag bean into the common bean enhance the crossability between tepary and common beans
topic embryo preservation
haplotypes
gene editing
conservación de embriones
haplotipos
edición de genes
ecology
url https://hdl.handle.net/10568/127311
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