Genetic analysis of resistance to bean leaf crumple virus identifies a candidate LRR-RLK gene
Bean leaf crumple virus (BLCrV) is a novel begomovirus (family Geminiviridae, genus Begomovirus) infecting common bean (Phaseolus vulgaris L.), threatening bean production in Latin America. Genetic resistance is required to ensure yield stability and reduce the use of insecticides, yet the available...
| Autores principales: | , , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Wiley
2023
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| Acceso en línea: | https://hdl.handle.net/10568/127298 |
| _version_ | 1855519974974554112 |
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| author | Ariza-Suárez, Daniel Keller, Beat Spescha, Anna Aparicio, Johan Steven Mayor, Victor Portilla Benavides, Ana Elisabeth Buendia, Hector Fabio Bueno, Juan Miguel Studer, Bruno Raatz, Bodo |
| author_browse | Aparicio, Johan Steven Ariza-Suárez, Daniel Buendia, Hector Fabio Bueno, Juan Miguel Keller, Beat Mayor, Victor Portilla Benavides, Ana Elisabeth Raatz, Bodo Spescha, Anna Studer, Bruno |
| author_facet | Ariza-Suárez, Daniel Keller, Beat Spescha, Anna Aparicio, Johan Steven Mayor, Victor Portilla Benavides, Ana Elisabeth Buendia, Hector Fabio Bueno, Juan Miguel Studer, Bruno Raatz, Bodo |
| author_sort | Ariza-Suárez, Daniel |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Bean leaf crumple virus (BLCrV) is a novel begomovirus (family Geminiviridae, genus Begomovirus) infecting common bean (Phaseolus vulgaris L.), threatening bean production in Latin America. Genetic resistance is required to ensure yield stability and reduce the use of insecticides, yet the available resistance sources are limited. In this study, three common bean populations containing a total of 558 genotypes were evaluated in different yield and BLCrV resistance trials under natural infection in the field. A genome‐wide association study identified the locus BLC7.1 on chromosome Pv07 at 3.31 Mbp, explaining 8 to 16% of the phenotypic variation for BLCrV resistance. In comparison, whole‐genome regression models explained 51 to 78% of the variation and identified the same region on Pv07 to confer resistance. The most significantly associated markers were located within the gene model Phvul.007G040400, which encodes a leucine‐rich repeat receptor‐like kinase subfamily III member and is likely to be involved in the innate immune response against the virus. The allelic diversity within this gene revealed five different haplotype groups, one of which was significantly associated with BLCrV resistance. As the same genome region was previously reported to be associated with resistance against other geminiviruses affecting common bean, our study highlights the role of previous breeding efforts for virus resistance in the accumulation of positive alleles against newly emerging viruses. In addition, we provide novel diagnostic single‐nucleotide polymorphism markers for marker‐assisted selection to exploit BLC7.1 for breeding against geminivirus diseases in one of the most important food crops worldwide. |
| format | Journal Article |
| id | CGSpace127298 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | CGSpace1272982025-11-11T17:45:21Z Genetic analysis of resistance to bean leaf crumple virus identifies a candidate LRR-RLK gene Ariza-Suárez, Daniel Keller, Beat Spescha, Anna Aparicio, Johan Steven Mayor, Victor Portilla Benavides, Ana Elisabeth Buendia, Hector Fabio Bueno, Juan Miguel Studer, Bruno Raatz, Bodo disease resistance marker-assisted selection genetic control resistencia a la enfermedad selección asistida por marcadores control genético cell biology genetics Bean leaf crumple virus (BLCrV) is a novel begomovirus (family Geminiviridae, genus Begomovirus) infecting common bean (Phaseolus vulgaris L.), threatening bean production in Latin America. Genetic resistance is required to ensure yield stability and reduce the use of insecticides, yet the available resistance sources are limited. In this study, three common bean populations containing a total of 558 genotypes were evaluated in different yield and BLCrV resistance trials under natural infection in the field. A genome‐wide association study identified the locus BLC7.1 on chromosome Pv07 at 3.31 Mbp, explaining 8 to 16% of the phenotypic variation for BLCrV resistance. In comparison, whole‐genome regression models explained 51 to 78% of the variation and identified the same region on Pv07 to confer resistance. The most significantly associated markers were located within the gene model Phvul.007G040400, which encodes a leucine‐rich repeat receptor‐like kinase subfamily III member and is likely to be involved in the innate immune response against the virus. The allelic diversity within this gene revealed five different haplotype groups, one of which was significantly associated with BLCrV resistance. As the same genome region was previously reported to be associated with resistance against other geminiviruses affecting common bean, our study highlights the role of previous breeding efforts for virus resistance in the accumulation of positive alleles against newly emerging viruses. In addition, we provide novel diagnostic single‐nucleotide polymorphism markers for marker‐assisted selection to exploit BLC7.1 for breeding against geminivirus diseases in one of the most important food crops worldwide. 2023-04 2023-01-17T14:30:51Z 2023-01-17T14:30:51Z Journal Article https://hdl.handle.net/10568/127298 en Open Access application/pdf Wiley Ariza-Suarez, D.; Keller, B.; Spescha, A.; Aparicio, J.S.; Mayor, V.; Portilla-Benavides, A.E.; Buendia, H.F.; Bueno, J.M.; Studer, B.; Raatz, B. (2022) Genetic analysis of resistance to bean leaf crumple virus identifies a candidate LRR-RLK gene. Plant Journal, Online first paper (2022-05-16) . ISSN: 0960-7412 |
| spellingShingle | disease resistance marker-assisted selection genetic control resistencia a la enfermedad selección asistida por marcadores control genético cell biology genetics Ariza-Suárez, Daniel Keller, Beat Spescha, Anna Aparicio, Johan Steven Mayor, Victor Portilla Benavides, Ana Elisabeth Buendia, Hector Fabio Bueno, Juan Miguel Studer, Bruno Raatz, Bodo Genetic analysis of resistance to bean leaf crumple virus identifies a candidate LRR-RLK gene |
| title | Genetic analysis of resistance to bean leaf crumple virus identifies a candidate LRR-RLK gene |
| title_full | Genetic analysis of resistance to bean leaf crumple virus identifies a candidate LRR-RLK gene |
| title_fullStr | Genetic analysis of resistance to bean leaf crumple virus identifies a candidate LRR-RLK gene |
| title_full_unstemmed | Genetic analysis of resistance to bean leaf crumple virus identifies a candidate LRR-RLK gene |
| title_short | Genetic analysis of resistance to bean leaf crumple virus identifies a candidate LRR-RLK gene |
| title_sort | genetic analysis of resistance to bean leaf crumple virus identifies a candidate lrr rlk gene |
| topic | disease resistance marker-assisted selection genetic control resistencia a la enfermedad selección asistida por marcadores control genético cell biology genetics |
| url | https://hdl.handle.net/10568/127298 |
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