SgNramp1, a plasma membrane-localized transporter, involves in manganese uptake in Stylosanthes guianensis
Transporters belonging to the natural resistance-associated macrophage protein (Nramp) family play important roles in metal uptake and homeostasis. Although Nramp members have been functionally characterized in plants, the role of Nramp in the important tropical forage legume Stylosanthes guianensis...
| Autores principales: | , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
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Frontiers Media
2022
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/127145 |
| _version_ | 1855522888910635008 |
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| author | Zou, Xiaoyan Huang, Rui Wang, Linjie Wang, Guihua Miao, Ye Rao, Idupulapati M. Liu, Guodao Chen, Zhijian |
| author_browse | Chen, Zhijian Huang, Rui Liu, Guodao Miao, Ye Rao, Idupulapati M. Wang, Guihua Wang, Linjie Zou, Xiaoyan |
| author_facet | Zou, Xiaoyan Huang, Rui Wang, Linjie Wang, Guihua Miao, Ye Rao, Idupulapati M. Liu, Guodao Chen, Zhijian |
| author_sort | Zou, Xiaoyan |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Transporters belonging to the natural resistance-associated macrophage protein (Nramp) family play important roles in metal uptake and homeostasis. Although Nramp members have been functionally characterized in plants, the role of Nramp in the important tropical forage legume Stylosanthes guianensis (stylo) is largely unknown. This study aimed to determine the responses of Nramp genes to metal stresses and investigate its metal transport activity in stylo. Five SgNramp genes were identified from stylo. Expression analysis showed that SgNramp genes exhibited tissue preferential expressions and diverse responses to metal stresses, especially for manganese (Mn), suggesting the involvement of SgNramps in the response of stylo to metal stresses. Of the five SgNramps, SgNramp1 displayed the highest expression in stylo roots. A close correlation between SgNramp1 expression and root Mn concentration was observed among nine stylo cultivars under Mn limited condition. The higher expression of SgNramp1 was correlated with a high Mn uptake in stylo. Subsequent subcellular localization analysis showed that SgNramp1 was localized to the plasma membrane. Furthermore, heterologous expression of SgNramp1 complemented the phenotype of the Mn uptake-defective yeast (Saccharomyces cerevisiae) mutant Dsmf1. Mn concentration in the yeast cells expressing SgNramp1 was higher than that of the empty vector control, suggesting the transport activity of SgNramp1 for Mn in yeast. Taken together, this study reveals that SgNramp1 is a plasma membrane–localized transporter responsible for Mn uptake in stylo. |
| format | Journal Article |
| id | CGSpace127145 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Frontiers Media |
| publisherStr | Frontiers Media |
| record_format | dspace |
| spelling | CGSpace1271452025-12-08T10:29:22Z SgNramp1, a plasma membrane-localized transporter, involves in manganese uptake in Stylosanthes guianensis Zou, Xiaoyan Huang, Rui Wang, Linjie Wang, Guihua Miao, Ye Rao, Idupulapati M. Liu, Guodao Chen, Zhijian homeostasis gene expression stylosanthes guianensis trace elements pcr Transporters belonging to the natural resistance-associated macrophage protein (Nramp) family play important roles in metal uptake and homeostasis. Although Nramp members have been functionally characterized in plants, the role of Nramp in the important tropical forage legume Stylosanthes guianensis (stylo) is largely unknown. This study aimed to determine the responses of Nramp genes to metal stresses and investigate its metal transport activity in stylo. Five SgNramp genes were identified from stylo. Expression analysis showed that SgNramp genes exhibited tissue preferential expressions and diverse responses to metal stresses, especially for manganese (Mn), suggesting the involvement of SgNramps in the response of stylo to metal stresses. Of the five SgNramps, SgNramp1 displayed the highest expression in stylo roots. A close correlation between SgNramp1 expression and root Mn concentration was observed among nine stylo cultivars under Mn limited condition. The higher expression of SgNramp1 was correlated with a high Mn uptake in stylo. Subsequent subcellular localization analysis showed that SgNramp1 was localized to the plasma membrane. Furthermore, heterologous expression of SgNramp1 complemented the phenotype of the Mn uptake-defective yeast (Saccharomyces cerevisiae) mutant Dsmf1. Mn concentration in the yeast cells expressing SgNramp1 was higher than that of the empty vector control, suggesting the transport activity of SgNramp1 for Mn in yeast. Taken together, this study reveals that SgNramp1 is a plasma membrane–localized transporter responsible for Mn uptake in stylo. 2022-10-06 2023-01-14T18:53:02Z 2023-01-14T18:53:02Z Journal Article https://hdl.handle.net/10568/127145 en Open Access application/pdf Frontiers Media Zou, X.; Huang, R.; Wang, L.; Wang, G.; Miao, Y.; Rao, I.; Liu, G.; Chen, Z. (2022) SgNramp1, a plasma membrane-localized transporter, involves in manganese uptake in Stylosanthes guianensis. Frontiers in Plant Science 13:1027551. ISSN: 1664-462X |
| spellingShingle | homeostasis gene expression stylosanthes guianensis trace elements pcr Zou, Xiaoyan Huang, Rui Wang, Linjie Wang, Guihua Miao, Ye Rao, Idupulapati M. Liu, Guodao Chen, Zhijian SgNramp1, a plasma membrane-localized transporter, involves in manganese uptake in Stylosanthes guianensis |
| title | SgNramp1, a plasma membrane-localized transporter, involves in manganese uptake in Stylosanthes guianensis |
| title_full | SgNramp1, a plasma membrane-localized transporter, involves in manganese uptake in Stylosanthes guianensis |
| title_fullStr | SgNramp1, a plasma membrane-localized transporter, involves in manganese uptake in Stylosanthes guianensis |
| title_full_unstemmed | SgNramp1, a plasma membrane-localized transporter, involves in manganese uptake in Stylosanthes guianensis |
| title_short | SgNramp1, a plasma membrane-localized transporter, involves in manganese uptake in Stylosanthes guianensis |
| title_sort | sgnramp1 a plasma membrane localized transporter involves in manganese uptake in stylosanthes guianensis |
| topic | homeostasis gene expression stylosanthes guianensis trace elements pcr |
| url | https://hdl.handle.net/10568/127145 |
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