Chlorophyll fluorescence imaging as a tool for evaluating disease resistance of common bean lines in the Western Amazon region of Colombia
The evaluation of disease resistance is considered an important aspect of phenotyping for crop improvement. Identification of advanced lines of the common bean with disease resistance contributes to improved grain yields. This study aimed to determine the response of the photosyn- thetic apparatus t...
| Autores principales: | , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
MDPI
2022
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/127036 |
| _version_ | 1855521147029815296 |
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| author | Suárez, Juan Carlos Vanegas, José Iván Contreras, Amara Tatiana Anzola, José Alexander Urban, Milan Oldřich Beebe, Stephen E. Rao, Idupulapati M. |
| author_browse | Anzola, José Alexander Beebe, Stephen E. Contreras, Amara Tatiana Rao, Idupulapati M. Suárez, Juan Carlos Urban, Milan Oldřich Vanegas, José Iván |
| author_facet | Suárez, Juan Carlos Vanegas, José Iván Contreras, Amara Tatiana Anzola, José Alexander Urban, Milan Oldřich Beebe, Stephen E. Rao, Idupulapati M. |
| author_sort | Suárez, Juan Carlos |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The evaluation of disease resistance is considered an important aspect of phenotyping for crop improvement. Identification of advanced lines of the common bean with disease resistance contributes to improved grain yields. This study aimed to determine the response of the photosyn- thetic apparatus to natural pathogen infection by using chlorophyll (Chla) fluorescence parameters and their relationship to the agronomic performance of 59 common bean lines and comparing the photosynthetic responses of naturally infected vs. healthy leaves. The study was conducted over two seasons under acid soil and high temperature conditions in the western Amazon region of Colombia. A disease susceptibility index (DSI) was developed and validated using chlorophyll a (Chla) fluorescence as a tool to identify Mesoamerican and Andean lines of common bean (Phaseolus vulgaris L.) that are resistant to pathogens. A negative effect on the functional status of the photo- synthetic apparatus was found with the presence of pathogen infection, a situation that allowed the identification of four typologies based on the DSI values ((i) moderately resistant; (ii) moderately susceptible; (iii) susceptible; and (iv) highly susceptible). Moderately resistant lines, five of them from the Mesoamerican gene pool (ALB 350, SMC 200, BFS 10, SER 16, SMN 27) and one from the Andean gene pool (DAB 295), allocated a higher proportion of energy to photochemical processes, which increased the rate of electron transfer resulting in a lower sensitivity to disease stress. This photosynthetic response was associated with lower values of DSI, which translated into an increase in the accumulation of dry matter accumulation in different plant organs (leaves, stem, pods and roots). Thus, DSI values based on chlorophyll fluorescence response to pathogen infection could serve as a phenotyping tool for evaluating advanced common bean lines. Six common bean lines (ALB 350, BFS 10, DAB 295, SER 16, SMC 200 and SMN 27) were identified as less sensitive to disease stress under field conditions in the western Amazon region of Colombia, and these could serve as useful parents for improving the common bean for multiple stress resistance. |
| format | Journal Article |
| id | CGSpace127036 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | CGSpace1270362025-12-08T10:29:22Z Chlorophyll fluorescence imaging as a tool for evaluating disease resistance of common bean lines in the Western Amazon region of Colombia Suárez, Juan Carlos Vanegas, José Iván Contreras, Amara Tatiana Anzola, José Alexander Urban, Milan Oldřich Beebe, Stephen E. Rao, Idupulapati M. acid soils agronomic characters performance assessment disease resistance dry matter yields heat-high temperature common beans phaseolus The evaluation of disease resistance is considered an important aspect of phenotyping for crop improvement. Identification of advanced lines of the common bean with disease resistance contributes to improved grain yields. This study aimed to determine the response of the photosyn- thetic apparatus to natural pathogen infection by using chlorophyll (Chla) fluorescence parameters and their relationship to the agronomic performance of 59 common bean lines and comparing the photosynthetic responses of naturally infected vs. healthy leaves. The study was conducted over two seasons under acid soil and high temperature conditions in the western Amazon region of Colombia. A disease susceptibility index (DSI) was developed and validated using chlorophyll a (Chla) fluorescence as a tool to identify Mesoamerican and Andean lines of common bean (Phaseolus vulgaris L.) that are resistant to pathogens. A negative effect on the functional status of the photo- synthetic apparatus was found with the presence of pathogen infection, a situation that allowed the identification of four typologies based on the DSI values ((i) moderately resistant; (ii) moderately susceptible; (iii) susceptible; and (iv) highly susceptible). Moderately resistant lines, five of them from the Mesoamerican gene pool (ALB 350, SMC 200, BFS 10, SER 16, SMN 27) and one from the Andean gene pool (DAB 295), allocated a higher proportion of energy to photochemical processes, which increased the rate of electron transfer resulting in a lower sensitivity to disease stress. This photosynthetic response was associated with lower values of DSI, which translated into an increase in the accumulation of dry matter accumulation in different plant organs (leaves, stem, pods and roots). Thus, DSI values based on chlorophyll fluorescence response to pathogen infection could serve as a phenotyping tool for evaluating advanced common bean lines. Six common bean lines (ALB 350, BFS 10, DAB 295, SER 16, SMC 200 and SMN 27) were identified as less sensitive to disease stress under field conditions in the western Amazon region of Colombia, and these could serve as useful parents for improving the common bean for multiple stress resistance. 2022-05-21 2023-01-13T10:09:42Z 2023-01-13T10:09:42Z Journal Article https://hdl.handle.net/10568/127036 en Open Access application/pdf MDPI Suárez, J.C.; Vanegas, J.I.; Contreras, A.T.; Anzola, J.A.; Urban, M.O.; Beebe, S.E.; Rao, I.M. (2022) Chlorophyll fluorescence imaging as a tool for evaluating disease resistance of common bean lines in the Western Amazon region of Colombia. Plants 11(10):1371. ISSN: 2223-7747 |
| spellingShingle | acid soils agronomic characters performance assessment disease resistance dry matter yields heat-high temperature common beans phaseolus Suárez, Juan Carlos Vanegas, José Iván Contreras, Amara Tatiana Anzola, José Alexander Urban, Milan Oldřich Beebe, Stephen E. Rao, Idupulapati M. Chlorophyll fluorescence imaging as a tool for evaluating disease resistance of common bean lines in the Western Amazon region of Colombia |
| title | Chlorophyll fluorescence imaging as a tool for evaluating disease resistance of common bean lines in the Western Amazon region of Colombia |
| title_full | Chlorophyll fluorescence imaging as a tool for evaluating disease resistance of common bean lines in the Western Amazon region of Colombia |
| title_fullStr | Chlorophyll fluorescence imaging as a tool for evaluating disease resistance of common bean lines in the Western Amazon region of Colombia |
| title_full_unstemmed | Chlorophyll fluorescence imaging as a tool for evaluating disease resistance of common bean lines in the Western Amazon region of Colombia |
| title_short | Chlorophyll fluorescence imaging as a tool for evaluating disease resistance of common bean lines in the Western Amazon region of Colombia |
| title_sort | chlorophyll fluorescence imaging as a tool for evaluating disease resistance of common bean lines in the western amazon region of colombia |
| topic | acid soils agronomic characters performance assessment disease resistance dry matter yields heat-high temperature common beans phaseolus |
| url | https://hdl.handle.net/10568/127036 |
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