QTL mapping of seedling and field resistance to stem rust in DAKIYE/Reichenbachii durum wheat population
Stem rust caused by the fungus Puccinia graminis f.sp. tritici Eriks. & E. Henn. (Pgt) threatens the global production of both durum wheat (Triticum turgidum L. ssp. durum (Desf.) Husnot) and common wheat (Triticum aestivum L.). The objective of this study was to evaluate a durum wheat recombinant i...
| Autores principales: | , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
2022
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/126707 |
| _version_ | 1855530233504989184 |
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| author | Megerssa, Shitaye Homma Ammar, Karim Acevedo, Maricelis Bergstrom, Gary Carlton Dreisigacker, Susanne Randhawa, Mandeep S. Brown-Guedira, Gina Ward, Brian Sorrells, Mark Earl |
| author_browse | Acevedo, Maricelis Ammar, Karim Bergstrom, Gary Carlton Brown-Guedira, Gina Dreisigacker, Susanne Megerssa, Shitaye Homma Randhawa, Mandeep S. Sorrells, Mark Earl Ward, Brian |
| author_facet | Megerssa, Shitaye Homma Ammar, Karim Acevedo, Maricelis Bergstrom, Gary Carlton Dreisigacker, Susanne Randhawa, Mandeep S. Brown-Guedira, Gina Ward, Brian Sorrells, Mark Earl |
| author_sort | Megerssa, Shitaye Homma |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Stem rust caused by the fungus Puccinia graminis f.sp. tritici Eriks. & E. Henn. (Pgt) threatens the global production of both durum wheat (Triticum turgidum L. ssp. durum (Desf.) Husnot) and common wheat (Triticum aestivum L.). The objective of this study was to evaluate a durum wheat recombinant inbred line (RIL) population from a cross between a susceptible parent ‘DAKIYE’ and a resistant parent ‘Reichenbachii’ developed by the International Center for the Improvement of Maize and Wheat (CIMMYT) 1) for seedling response to races JRCQC and TTRTF and 2) for field response to a bulk of the current Pgt races prevalent in Ethiopia and Kenya and 3) to map loci associated with seedling and field resistances in this population. A total of 224 RILs along with their parents were evaluated at the seedling stage in the Ethiopian Institute for Agricultural Research greenhouse at Debre Zeit, Ethiopia and in the EIAR and KALRO fields in Ethiopia and Kenya, for two seasons from 2019 to 2020. The lines were genotyped using the genotyping-by-sequencing approach. A total of 843 single nucleotide polymorphism markers for 175 lines were used for quantitative trait locus (QTL) analyses. Composite interval mapping (CIM) identified three QTL on chromosomes 3B, 4B and 7B contributed by the resistant parent. The QTL on chromosome 3B was identified at all growth stages and it explained 11.8%, 6.5%, 6.4% and 15.3% of the phenotypic variation for responses to races JRCQC, TTRTF and in the field trials ETMS19 and KNMS19, respectively. The power to identify additional QTL in this population was limited by the number of high-quality markers, since several markers with segregation distortion were eliminated. A cytological study is needed to understand the presence of chromosomal rearrangements. Future evaluations of additional durum lines and RIL families identification of durable adult plant resistance sources is crucial for breeding stem rust resistance in durum wheat in the future. |
| format | Journal Article |
| id | CGSpace126707 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| record_format | dspace |
| spelling | CGSpace1267072025-11-06T13:06:52Z QTL mapping of seedling and field resistance to stem rust in DAKIYE/Reichenbachii durum wheat population Megerssa, Shitaye Homma Ammar, Karim Acevedo, Maricelis Bergstrom, Gary Carlton Dreisigacker, Susanne Randhawa, Mandeep S. Brown-Guedira, Gina Ward, Brian Sorrells, Mark Earl breeding chromosomes genetic markers genotyping greenhouses maize phenotypic variation plant response quantitative analysis quantitative trait loci mapping seedlings single nucleotide polymorphism stem rust wheat basidiomycota disease resistance genetics microbiology plant breeding Stem rust caused by the fungus Puccinia graminis f.sp. tritici Eriks. & E. Henn. (Pgt) threatens the global production of both durum wheat (Triticum turgidum L. ssp. durum (Desf.) Husnot) and common wheat (Triticum aestivum L.). The objective of this study was to evaluate a durum wheat recombinant inbred line (RIL) population from a cross between a susceptible parent ‘DAKIYE’ and a resistant parent ‘Reichenbachii’ developed by the International Center for the Improvement of Maize and Wheat (CIMMYT) 1) for seedling response to races JRCQC and TTRTF and 2) for field response to a bulk of the current Pgt races prevalent in Ethiopia and Kenya and 3) to map loci associated with seedling and field resistances in this population. A total of 224 RILs along with their parents were evaluated at the seedling stage in the Ethiopian Institute for Agricultural Research greenhouse at Debre Zeit, Ethiopia and in the EIAR and KALRO fields in Ethiopia and Kenya, for two seasons from 2019 to 2020. The lines were genotyped using the genotyping-by-sequencing approach. A total of 843 single nucleotide polymorphism markers for 175 lines were used for quantitative trait locus (QTL) analyses. Composite interval mapping (CIM) identified three QTL on chromosomes 3B, 4B and 7B contributed by the resistant parent. The QTL on chromosome 3B was identified at all growth stages and it explained 11.8%, 6.5%, 6.4% and 15.3% of the phenotypic variation for responses to races JRCQC, TTRTF and in the field trials ETMS19 and KNMS19, respectively. The power to identify additional QTL in this population was limited by the number of high-quality markers, since several markers with segregation distortion were eliminated. A cytological study is needed to understand the presence of chromosomal rearrangements. Future evaluations of additional durum lines and RIL families identification of durable adult plant resistance sources is crucial for breeding stem rust resistance in durum wheat in the future. 2022-10-06 2023-01-09T10:10:38Z 2023-01-09T10:10:38Z Journal Article https://hdl.handle.net/10568/126707 en Open Access application/pdf Megerssa, S. H., Ammar, K., Acevedo, M., Bergstrom, G. C., Dreisigacker, S., Randhawa, M., Brown-Guedira, G., Ward, B., & Sorrells, M. E. (2022). QTL mapping of seedling and field resistance to stem rust in DAKIYE/Reichenbachii durum wheat population. PLOS ONE, 17(10), e0273993. https://doi.org/10.1371/journal.pone.0273993 |
| spellingShingle | breeding chromosomes genetic markers genotyping greenhouses maize phenotypic variation plant response quantitative analysis quantitative trait loci mapping seedlings single nucleotide polymorphism stem rust wheat basidiomycota disease resistance genetics microbiology plant breeding Megerssa, Shitaye Homma Ammar, Karim Acevedo, Maricelis Bergstrom, Gary Carlton Dreisigacker, Susanne Randhawa, Mandeep S. Brown-Guedira, Gina Ward, Brian Sorrells, Mark Earl QTL mapping of seedling and field resistance to stem rust in DAKIYE/Reichenbachii durum wheat population |
| title | QTL mapping of seedling and field resistance to stem rust in DAKIYE/Reichenbachii durum wheat population |
| title_full | QTL mapping of seedling and field resistance to stem rust in DAKIYE/Reichenbachii durum wheat population |
| title_fullStr | QTL mapping of seedling and field resistance to stem rust in DAKIYE/Reichenbachii durum wheat population |
| title_full_unstemmed | QTL mapping of seedling and field resistance to stem rust in DAKIYE/Reichenbachii durum wheat population |
| title_short | QTL mapping of seedling and field resistance to stem rust in DAKIYE/Reichenbachii durum wheat population |
| title_sort | qtl mapping of seedling and field resistance to stem rust in dakiye reichenbachii durum wheat population |
| topic | breeding chromosomes genetic markers genotyping greenhouses maize phenotypic variation plant response quantitative analysis quantitative trait loci mapping seedlings single nucleotide polymorphism stem rust wheat basidiomycota disease resistance genetics microbiology plant breeding |
| url | https://hdl.handle.net/10568/126707 |
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