Identification of a novel stripe rust resistance gene from the European winter wheat cultivar ‘Acienda’: A step towards rust proofing wheat cultivation

All stage resistance to stripe rust races prevalent in India was investigated in the European winter wheat cultivar ‘Acienda’. In order to dissect the genetic basis of the resistance, a backcross population was developed between ‘Acienda’ and the stripe rust susceptible Indian spring wheat cultivar...

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Autores principales: Grover, Gomti, Sharma, Achla, Mackay, Ian, Srivastava, Puja, Kaur, Satinder, Kaur, Jaspal, Burridge, Amanda, Allen, Sacha Przewieslik, Bentley, Alison R., Chhuneja, Parveen, Bains, N.S.
Formato: Journal Article
Lenguaje:Inglés
Publicado: 2022
Materias:
Acceso en línea:https://hdl.handle.net/10568/126704
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author Grover, Gomti
Sharma, Achla
Mackay, Ian
Srivastava, Puja
Kaur, Satinder
Kaur, Jaspal
Burridge, Amanda
Allen, Sacha Przewieslik
Bentley, Alison R.
Chhuneja, Parveen
Bains, N.S.
author_browse Allen, Sacha Przewieslik
Bains, N.S.
Bentley, Alison R.
Burridge, Amanda
Chhuneja, Parveen
Grover, Gomti
Kaur, Jaspal
Kaur, Satinder
Mackay, Ian
Sharma, Achla
Srivastava, Puja
author_facet Grover, Gomti
Sharma, Achla
Mackay, Ian
Srivastava, Puja
Kaur, Satinder
Kaur, Jaspal
Burridge, Amanda
Allen, Sacha Przewieslik
Bentley, Alison R.
Chhuneja, Parveen
Bains, N.S.
author_sort Grover, Gomti
collection Repository of Agricultural Research Outputs (CGSpace)
description All stage resistance to stripe rust races prevalent in India was investigated in the European winter wheat cultivar ‘Acienda’. In order to dissect the genetic basis of the resistance, a backcross population was developed between ‘Acienda’ and the stripe rust susceptible Indian spring wheat cultivar ‘HD 2967’. Inheritance studies revealed segregation for a dominant resistant gene. High density SNP genotyping was used to map stripe rust resistance and marker regression analysis located stripe rust resistance to the distal end of wheat chromosome 1A. Interval mapping located this region between the SNP markers AX-95162217 and AX-94540853, at a LOD score of 15.83 with a phenotypic contribution of 60%. This major stripe rust resistance locus from ‘Acienda’ has been temporarily designated as Yraci. A candidate gene search in the 2.76 Mb region carrying Yraci on chromosome 1A identified 18 NBS-LRR genes based on wheat RefSeqv1.0 annotations. Our results indicate that as there is no major gene reported in the Yraci chromosome region, it is likely to be a novel stripe rust resistance locus and offers potential for deployment, using the identified markers, to confer all stage stripe rust resistance.
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spelling CGSpace1267042025-11-06T13:02:51Z Identification of a novel stripe rust resistance gene from the European winter wheat cultivar ‘Acienda’: A step towards rust proofing wheat cultivation Grover, Gomti Sharma, Achla Mackay, Ian Srivastava, Puja Kaur, Satinder Kaur, Jaspal Burridge, Amanda Allen, Sacha Przewieslik Bentley, Alison R. Chhuneja, Parveen Bains, N.S. wheat loci chromosome mapping single nucleotide polymorphism plant breeding genotyping All stage resistance to stripe rust races prevalent in India was investigated in the European winter wheat cultivar ‘Acienda’. In order to dissect the genetic basis of the resistance, a backcross population was developed between ‘Acienda’ and the stripe rust susceptible Indian spring wheat cultivar ‘HD 2967’. Inheritance studies revealed segregation for a dominant resistant gene. High density SNP genotyping was used to map stripe rust resistance and marker regression analysis located stripe rust resistance to the distal end of wheat chromosome 1A. Interval mapping located this region between the SNP markers AX-95162217 and AX-94540853, at a LOD score of 15.83 with a phenotypic contribution of 60%. This major stripe rust resistance locus from ‘Acienda’ has been temporarily designated as Yraci. A candidate gene search in the 2.76 Mb region carrying Yraci on chromosome 1A identified 18 NBS-LRR genes based on wheat RefSeqv1.0 annotations. Our results indicate that as there is no major gene reported in the Yraci chromosome region, it is likely to be a novel stripe rust resistance locus and offers potential for deployment, using the identified markers, to confer all stage stripe rust resistance. 2022-02-16 2023-01-09T10:10:24Z 2023-01-09T10:10:24Z Journal Article https://hdl.handle.net/10568/126704 en Open Access application/pdf Grover, G., Sharma, A., Mackay, I., Srivastava, P., Kaur, S., Kaur, J., Burridge, A., Allen, S. P., Bentley, A. R., Chhuneja, P., & Bains, N. S. (2022). Identification of a novel stripe rust resistance gene from the European winter wheat cultivar ‘Acienda’: A step towards rust proofing wheat cultivation. PLOS ONE, 17(2), e0264027. https://doi.org/10.1371/journal.pone.0264027
spellingShingle wheat
loci
chromosome mapping
single nucleotide polymorphism
plant breeding
genotyping
Grover, Gomti
Sharma, Achla
Mackay, Ian
Srivastava, Puja
Kaur, Satinder
Kaur, Jaspal
Burridge, Amanda
Allen, Sacha Przewieslik
Bentley, Alison R.
Chhuneja, Parveen
Bains, N.S.
Identification of a novel stripe rust resistance gene from the European winter wheat cultivar ‘Acienda’: A step towards rust proofing wheat cultivation
title Identification of a novel stripe rust resistance gene from the European winter wheat cultivar ‘Acienda’: A step towards rust proofing wheat cultivation
title_full Identification of a novel stripe rust resistance gene from the European winter wheat cultivar ‘Acienda’: A step towards rust proofing wheat cultivation
title_fullStr Identification of a novel stripe rust resistance gene from the European winter wheat cultivar ‘Acienda’: A step towards rust proofing wheat cultivation
title_full_unstemmed Identification of a novel stripe rust resistance gene from the European winter wheat cultivar ‘Acienda’: A step towards rust proofing wheat cultivation
title_short Identification of a novel stripe rust resistance gene from the European winter wheat cultivar ‘Acienda’: A step towards rust proofing wheat cultivation
title_sort identification of a novel stripe rust resistance gene from the european winter wheat cultivar acienda a step towards rust proofing wheat cultivation
topic wheat
loci
chromosome mapping
single nucleotide polymorphism
plant breeding
genotyping
url https://hdl.handle.net/10568/126704
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