Genetic characterization of oleaginous bottle gourd (Lagenaria siceraria) germplasm from Côte d'Ivoire using agromorphological and molecular markers
Being difficult to regenerate and maintain the seeds, the oleaginous bottle gourd was investigated using nine agromorphological traits and 31 amplified fragment length polymorphism (AFLP) markers. Specifically, the study was conducted to determine the intra-specific variability of a total of 173 acc...
| Autores principales: | , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
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Cambridge University Press
2022
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| Acceso en línea: | https://hdl.handle.net/10568/126666 |
| _version_ | 1855525827043655680 |
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| author | Gbotto, A.A. Yao, N.K. Kitavi, M. Osama, S.K. Habimana, R. Koffi, K.K. Zoro Bi, I.A. |
| author_browse | Gbotto, A.A. Habimana, R. Kitavi, M. Koffi, K.K. Osama, S.K. Yao, N.K. Zoro Bi, I.A. |
| author_facet | Gbotto, A.A. Yao, N.K. Kitavi, M. Osama, S.K. Habimana, R. Koffi, K.K. Zoro Bi, I.A. |
| author_sort | Gbotto, A.A. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Being difficult to regenerate and maintain the seeds, the oleaginous bottle gourd was investigated using nine agromorphological traits and 31 amplified fragment length polymorphism (AFLP) markers. Specifically, the study was conducted to determine the intra-specific variability of a total of 173 accessions, which were identified from five agro-ecological regions from Côte d'Ivoire (Centre, East, North and South). Then, the genetic diversity and relationships within accessions were studied using AFLP markers. This characterization using both morphological and AFLP markers was realized in order to ultimately build a reliable core collection. The discriminant analysis, using nine quantitative traits, reveals plant length and seeds number per fruit as discriminating characteristics. From the accessions used for the agromorphological study, 148 were able to be differentiated by the AFLP markers. A range of 52 to 113 bands were amplified per primer combination. As revealed by the analysis of molecular variance (AMOVA), 28% of the total variation resides among accessions and 72% occurs within populations. The AMOVA computed in order to differentiate cultivars, displayed the same trends when no prior grouping of accessions was considered. The differentiation within cultivar (97%) was more than that, among cultivars (3%). Tree topologies inferred by neighbour-joining analysis reflected no clear cut off grouping. |
| format | Journal Article |
| id | CGSpace126666 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Cambridge University Press |
| publisherStr | Cambridge University Press |
| record_format | dspace |
| spelling | CGSpace1266662025-01-27T15:00:52Z Genetic characterization of oleaginous bottle gourd (Lagenaria siceraria) germplasm from Côte d'Ivoire using agromorphological and molecular markers Gbotto, A.A. Yao, N.K. Kitavi, M. Osama, S.K. Habimana, R. Koffi, K.K. Zoro Bi, I.A. core collections genetic variation lagenaria siceraria germplasm Being difficult to regenerate and maintain the seeds, the oleaginous bottle gourd was investigated using nine agromorphological traits and 31 amplified fragment length polymorphism (AFLP) markers. Specifically, the study was conducted to determine the intra-specific variability of a total of 173 accessions, which were identified from five agro-ecological regions from Côte d'Ivoire (Centre, East, North and South). Then, the genetic diversity and relationships within accessions were studied using AFLP markers. This characterization using both morphological and AFLP markers was realized in order to ultimately build a reliable core collection. The discriminant analysis, using nine quantitative traits, reveals plant length and seeds number per fruit as discriminating characteristics. From the accessions used for the agromorphological study, 148 were able to be differentiated by the AFLP markers. A range of 52 to 113 bands were amplified per primer combination. As revealed by the analysis of molecular variance (AMOVA), 28% of the total variation resides among accessions and 72% occurs within populations. The AMOVA computed in order to differentiate cultivars, displayed the same trends when no prior grouping of accessions was considered. The differentiation within cultivar (97%) was more than that, among cultivars (3%). Tree topologies inferred by neighbour-joining analysis reflected no clear cut off grouping. 2022-04 2023-01-06T17:03:56Z 2023-01-06T17:03:56Z Journal Article https://hdl.handle.net/10568/126666 en Limited Access Cambridge University Press Gbotto, A.A.; Yao, N.K.; Kitavi, M.; Osama, S.K.; Habimana, R.; Koffi, K.K.; Zoro Bi, I.A. 2022. Genetic characterization of oleaginous bottle gourd (Lagenaria siceraria) germplasm from Côte d’Ivoire using agromorphological and molecular markers. Plant Genetic Resources: Characterization and Utilization. ISSN 1479-263X. 1–12. |
| spellingShingle | core collections genetic variation lagenaria siceraria germplasm Gbotto, A.A. Yao, N.K. Kitavi, M. Osama, S.K. Habimana, R. Koffi, K.K. Zoro Bi, I.A. Genetic characterization of oleaginous bottle gourd (Lagenaria siceraria) germplasm from Côte d'Ivoire using agromorphological and molecular markers |
| title | Genetic characterization of oleaginous bottle gourd (Lagenaria siceraria) germplasm from Côte d'Ivoire using agromorphological and molecular markers |
| title_full | Genetic characterization of oleaginous bottle gourd (Lagenaria siceraria) germplasm from Côte d'Ivoire using agromorphological and molecular markers |
| title_fullStr | Genetic characterization of oleaginous bottle gourd (Lagenaria siceraria) germplasm from Côte d'Ivoire using agromorphological and molecular markers |
| title_full_unstemmed | Genetic characterization of oleaginous bottle gourd (Lagenaria siceraria) germplasm from Côte d'Ivoire using agromorphological and molecular markers |
| title_short | Genetic characterization of oleaginous bottle gourd (Lagenaria siceraria) germplasm from Côte d'Ivoire using agromorphological and molecular markers |
| title_sort | genetic characterization of oleaginous bottle gourd lagenaria siceraria germplasm from cote d ivoire using agromorphological and molecular markers |
| topic | core collections genetic variation lagenaria siceraria germplasm |
| url | https://hdl.handle.net/10568/126666 |
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