Adult plant stem rust resistance in durum wheat Glossy Huguenot: Mapping, marker development and validation
An F3 population from a Glossy Huguenot (GH)/Bansi cross used in a previous Australian study was advanced to F6 for molecular mapping of adult plant stem rust resistance. Maturity differences among F6 lines confounded assessments of stem rust response. GH was crossed with a stem rust susceptible F6...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Springer
2022
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/126590 |
| _version_ | 1855526710441672704 |
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| author | Mago, Rohit Chunhong Chen Xiaodi Xia Whan, Alex Forrest, Kerrie L. Basnet, Bhoja Raj Perera, Geetha Chandramohan, Sutha Randhawa, Mandeep S. Hayden, Matthew Bansal, Urmil Huerta Espino, Julio Singh, Ravi P. Bariana, Harbans S. Lagudah, Evans S. |
| author_browse | Bansal, Urmil Bariana, Harbans S. Basnet, Bhoja Raj Chandramohan, Sutha Chunhong Chen Forrest, Kerrie L. Hayden, Matthew Huerta Espino, Julio Lagudah, Evans S. Mago, Rohit Perera, Geetha Randhawa, Mandeep S. Singh, Ravi P. Whan, Alex Xiaodi Xia |
| author_facet | Mago, Rohit Chunhong Chen Xiaodi Xia Whan, Alex Forrest, Kerrie L. Basnet, Bhoja Raj Perera, Geetha Chandramohan, Sutha Randhawa, Mandeep S. Hayden, Matthew Bansal, Urmil Huerta Espino, Julio Singh, Ravi P. Bariana, Harbans S. Lagudah, Evans S. |
| author_sort | Mago, Rohit |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | An F3 population from a Glossy Huguenot (GH)/Bansi cross used in a previous Australian study was advanced to F6 for molecular mapping of adult plant stem rust resistance. Maturity differences among F6 lines confounded assessments of stem rust response. GH was crossed with a stem rust susceptible F6 recombinant inbred line (RIL), GHB14 (M14), with similar maturity and an F6:7 population was developed through single seed descent method. F7 and F8 RILs were tested along with the parents at different locations. The F6 individual plants and both parents were genotyped using the 90 K single nucleotide polymorphism (SNP) wheat array. Stem rust resistance QTL on the long arms of chromosomes 1B (QSrGH.cs-1BL) and 2A (QSrGH.cs-2AL) were detected. QSrGH.cs-1BL and QSrGH.cs-2AL were both contributed by GH and explained 22% and 18% adult plant stem rust response variation, respectively, among GH/M14 RIL population. RILs carrying combinations of these QTL reduced more than 14% stem rust severity compared to those that possessed QSrGH.cs-1BL and QSrGH.cs-2AL individually. QSrGH.cs1BL was demonstrated to be the same as Sr58/Lr46/Yr29/Pm39 through marker genotyping. Lines lacking QSrGH.cs-1BL were used to Mendelise QSrGH.cs-2AL. Based on genomic locations of previously catalogued stem rust resistance genes and the QSrGH.cs-2AL map, it appeared to represent a new APR locus and was permanently named Sr63. SNP markers associated with Sr63 were converted to kompetetive allele-specific PCR (KASP) assays and were validated on a set of durum cultivars. |
| format | Journal Article |
| id | CGSpace126590 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace1265902023-12-08T19:36:04Z Adult plant stem rust resistance in durum wheat Glossy Huguenot: Mapping, marker development and validation Mago, Rohit Chunhong Chen Xiaodi Xia Whan, Alex Forrest, Kerrie L. Basnet, Bhoja Raj Perera, Geetha Chandramohan, Sutha Randhawa, Mandeep S. Hayden, Matthew Bansal, Urmil Huerta Espino, Julio Singh, Ravi P. Bariana, Harbans S. Lagudah, Evans S. genes plants hard wheat seeds rusts disease resistance alleles An F3 population from a Glossy Huguenot (GH)/Bansi cross used in a previous Australian study was advanced to F6 for molecular mapping of adult plant stem rust resistance. Maturity differences among F6 lines confounded assessments of stem rust response. GH was crossed with a stem rust susceptible F6 recombinant inbred line (RIL), GHB14 (M14), with similar maturity and an F6:7 population was developed through single seed descent method. F7 and F8 RILs were tested along with the parents at different locations. The F6 individual plants and both parents were genotyped using the 90 K single nucleotide polymorphism (SNP) wheat array. Stem rust resistance QTL on the long arms of chromosomes 1B (QSrGH.cs-1BL) and 2A (QSrGH.cs-2AL) were detected. QSrGH.cs-1BL and QSrGH.cs-2AL were both contributed by GH and explained 22% and 18% adult plant stem rust response variation, respectively, among GH/M14 RIL population. RILs carrying combinations of these QTL reduced more than 14% stem rust severity compared to those that possessed QSrGH.cs-1BL and QSrGH.cs-2AL individually. QSrGH.cs1BL was demonstrated to be the same as Sr58/Lr46/Yr29/Pm39 through marker genotyping. Lines lacking QSrGH.cs-1BL were used to Mendelise QSrGH.cs-2AL. Based on genomic locations of previously catalogued stem rust resistance genes and the QSrGH.cs-2AL map, it appeared to represent a new APR locus and was permanently named Sr63. SNP markers associated with Sr63 were converted to kompetetive allele-specific PCR (KASP) assays and were validated on a set of durum cultivars. 2022-05 2023-01-05T08:33:10Z 2023-01-05T08:33:10Z Journal Article https://hdl.handle.net/10568/126590 en Limited Access Springer Mago, R., Chen, C., Xia, X., Whan, A., Forrest, K., Basnet, B.R., Perera, G., Chandramohan, S., Randhawa, M., Hayden, M., Bansal, U., Huerta-Espino, J., Singh, R.P., Bariana, H. and Lagudah, E. 2022. Adult plant stem rust resistance in durum wheat Glossy Huguenot: Mapping, marker develop-ment and validation. Theoretical and Applied Genetics 135(5):1541–1550. |
| spellingShingle | genes plants hard wheat seeds rusts disease resistance alleles Mago, Rohit Chunhong Chen Xiaodi Xia Whan, Alex Forrest, Kerrie L. Basnet, Bhoja Raj Perera, Geetha Chandramohan, Sutha Randhawa, Mandeep S. Hayden, Matthew Bansal, Urmil Huerta Espino, Julio Singh, Ravi P. Bariana, Harbans S. Lagudah, Evans S. Adult plant stem rust resistance in durum wheat Glossy Huguenot: Mapping, marker development and validation |
| title | Adult plant stem rust resistance in durum wheat Glossy Huguenot: Mapping, marker development and validation |
| title_full | Adult plant stem rust resistance in durum wheat Glossy Huguenot: Mapping, marker development and validation |
| title_fullStr | Adult plant stem rust resistance in durum wheat Glossy Huguenot: Mapping, marker development and validation |
| title_full_unstemmed | Adult plant stem rust resistance in durum wheat Glossy Huguenot: Mapping, marker development and validation |
| title_short | Adult plant stem rust resistance in durum wheat Glossy Huguenot: Mapping, marker development and validation |
| title_sort | adult plant stem rust resistance in durum wheat glossy huguenot mapping marker development and validation |
| topic | genes plants hard wheat seeds rusts disease resistance alleles |
| url | https://hdl.handle.net/10568/126590 |
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