Preliminary study on the seasonal questing of Ixodes ricinus group ticks in Ain Draham forest (north-western Tunisia) with analyses of their phylogenetic diversity
The present study aimed to investigate the activity dynamics of Ixodes ricinus group ticks in a forest located in north-western Tunisia (Aïn Draham, Jendouba District) and assess the variation of abiotic factors (temperature, Normalized Difference Vegetation Index and relative humidity) during one y...
| Autores principales: | , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2023
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/126579 |
| _version_ | 1855532155360247808 |
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| author | Elati, Khawla Khammasi Khbou, Médiha Kahl, Olaf Mwacharo, Joram Al-Shamaa, Khaled Rekik, Mourad Darghouth, Mohamed Aziz Gharbi, Mohamed |
| author_browse | Al-Shamaa, Khaled Darghouth, Mohamed Aziz Elati, Khawla Gharbi, Mohamed Kahl, Olaf Khammasi Khbou, Médiha Mwacharo, Joram Rekik, Mourad |
| author_facet | Elati, Khawla Khammasi Khbou, Médiha Kahl, Olaf Mwacharo, Joram Al-Shamaa, Khaled Rekik, Mourad Darghouth, Mohamed Aziz Gharbi, Mohamed |
| author_sort | Elati, Khawla |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The present study aimed to investigate the activity dynamics of Ixodes ricinus group ticks in a forest located in north-western Tunisia (Aïn Draham, Jendouba District) and assess the variation of abiotic factors (temperature, Normalized Difference Vegetation Index and relative humidity) during one year survey from September 2016 to August 2017 using the dragging sampling method. A total of 116 questing ticks was collected from the vegetation consisting of 47 adults (19 females and 28 males, sex ratio M:F = 1.47), 45 nymphs and 24 larvae representing 40.5, 38.8 and 20.7% of the total collected specimens, respectively. Adult I. ricinus were collected during October–May, nymphs during May–August and larvae during July–September. There were statistically significant correlations between adult tick numbers and mean daily relative humidity (Pearson r = 0.77; p = 0.003) and mean daily temperature (r = −0.74; p = 0.006).
The comparison of 16S rDNA sequences from 20 adult ticks of approximately 444 bp length showed variability among 11 sequences. There was a low genetic variability (<1%) among the I. ricinus isolates collected from the forest. The amplicons showed >99% identity with I. ricinus and Ixodes inopinatus sequences from different countries and published in GenBank. These results should be complemented by further surveys in other Tunisian regions to better understand the influence of environmental factors on the biology of I. ricinus and the occurrence of sympatric I. inopinatus ticks. Different molecular markers should be used for better understanding of their taxonomic status. |
| format | Journal Article |
| id | CGSpace126579 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | CGSpace1265792026-01-21T11:56:52Z Preliminary study on the seasonal questing of Ixodes ricinus group ticks in Ain Draham forest (north-western Tunisia) with analyses of their phylogenetic diversity Elati, Khawla Khammasi Khbou, Médiha Kahl, Olaf Mwacharo, Joram Al-Shamaa, Khaled Rekik, Mourad Darghouth, Mohamed Aziz Gharbi, Mohamed food security health nutrition tunisia abiotic factors ixodes ricinus goal 3 good health and well-being abundance activity dynamics The present study aimed to investigate the activity dynamics of Ixodes ricinus group ticks in a forest located in north-western Tunisia (Aïn Draham, Jendouba District) and assess the variation of abiotic factors (temperature, Normalized Difference Vegetation Index and relative humidity) during one year survey from September 2016 to August 2017 using the dragging sampling method. A total of 116 questing ticks was collected from the vegetation consisting of 47 adults (19 females and 28 males, sex ratio M:F = 1.47), 45 nymphs and 24 larvae representing 40.5, 38.8 and 20.7% of the total collected specimens, respectively. Adult I. ricinus were collected during October–May, nymphs during May–August and larvae during July–September. There were statistically significant correlations between adult tick numbers and mean daily relative humidity (Pearson r = 0.77; p = 0.003) and mean daily temperature (r = −0.74; p = 0.006). The comparison of 16S rDNA sequences from 20 adult ticks of approximately 444 bp length showed variability among 11 sequences. There was a low genetic variability (<1%) among the I. ricinus isolates collected from the forest. The amplicons showed >99% identity with I. ricinus and Ixodes inopinatus sequences from different countries and published in GenBank. These results should be complemented by further surveys in other Tunisian regions to better understand the influence of environmental factors on the biology of I. ricinus and the occurrence of sympatric I. inopinatus ticks. Different molecular markers should be used for better understanding of their taxonomic status. 2023-01-04T21:22:46Z 2023-01-04T21:22:46Z Journal Article https://hdl.handle.net/10568/126579 en Limited Access Elsevier Khawla Elati, Médiha Khammasi Khbou, Olaf Kahl, Joram Mwacharo, Khaled Al-Shamaa, Mourad Rekik, Mohamed Aziz Darghouth, Mohamed Gharbi. (23/9/2022). Preliminary study on the seasonal questing of Ixodes ricinus group ticks in Ain Draham forest (north-western Tunisia) with analyses of their phylogenetic diversity. Veterinary Parasitology: Regional Studies and Reports, 36. |
| spellingShingle | food security health nutrition tunisia abiotic factors ixodes ricinus goal 3 good health and well-being abundance activity dynamics Elati, Khawla Khammasi Khbou, Médiha Kahl, Olaf Mwacharo, Joram Al-Shamaa, Khaled Rekik, Mourad Darghouth, Mohamed Aziz Gharbi, Mohamed Preliminary study on the seasonal questing of Ixodes ricinus group ticks in Ain Draham forest (north-western Tunisia) with analyses of their phylogenetic diversity |
| title | Preliminary study on the seasonal questing of Ixodes ricinus group ticks in Ain Draham forest (north-western Tunisia) with analyses of their phylogenetic diversity |
| title_full | Preliminary study on the seasonal questing of Ixodes ricinus group ticks in Ain Draham forest (north-western Tunisia) with analyses of their phylogenetic diversity |
| title_fullStr | Preliminary study on the seasonal questing of Ixodes ricinus group ticks in Ain Draham forest (north-western Tunisia) with analyses of their phylogenetic diversity |
| title_full_unstemmed | Preliminary study on the seasonal questing of Ixodes ricinus group ticks in Ain Draham forest (north-western Tunisia) with analyses of their phylogenetic diversity |
| title_short | Preliminary study on the seasonal questing of Ixodes ricinus group ticks in Ain Draham forest (north-western Tunisia) with analyses of their phylogenetic diversity |
| title_sort | preliminary study on the seasonal questing of ixodes ricinus group ticks in ain draham forest north western tunisia with analyses of their phylogenetic diversity |
| topic | food security health nutrition tunisia abiotic factors ixodes ricinus goal 3 good health and well-being abundance activity dynamics |
| url | https://hdl.handle.net/10568/126579 |
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