First insights into the microbiome of Tunisian Hyalomma ticks gained through nextgeneration sequencing with a special focus on H. scupense
Ticks are one of the most important vectors of several pathogens affecting humans and animals. In addition to pathogens, ticks carry diverse microbiota of symbiotic and commensal microorganisms. In this study, we have investigated the first Tunisian insight into the microbial composition of the most...
| Main Authors: | , , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
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Public Library of Science (PLOS ONE)
2023
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| Online Access: | https://hdl.handle.net/10568/126568 |
| _version_ | 1855514854275678208 |
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| author | Ben Yedem, Hayet Lekired, Abdelmalek Mhadhbi, Moez Dhibi, Mokhtar Romdhane, Rihab Chaari, Soufiene Rekik, Mourad Ouzari, Hadda-Imene Hajji, Tarek Darghouth, Mohamed Aziz |
| author_browse | Ben Yedem, Hayet Chaari, Soufiene Darghouth, Mohamed Aziz Dhibi, Mokhtar Hajji, Tarek Lekired, Abdelmalek Mhadhbi, Moez Ouzari, Hadda-Imene Rekik, Mourad Romdhane, Rihab |
| author_facet | Ben Yedem, Hayet Lekired, Abdelmalek Mhadhbi, Moez Dhibi, Mokhtar Romdhane, Rihab Chaari, Soufiene Rekik, Mourad Ouzari, Hadda-Imene Hajji, Tarek Darghouth, Mohamed Aziz |
| author_sort | Ben Yedem, Hayet |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Ticks are one of the most important vectors of several pathogens affecting humans and animals. In addition to pathogens, ticks carry diverse microbiota of symbiotic and commensal microorganisms. In this study, we have investigated the first Tunisian insight into the microbial composition of the most dominant Hyalomma species infesting Tunisian cattle and explored the relative contribution of tick sex, life stage, and species to the diversity, richness and bacterial species of tick microbiome. In this regard, next generation sequencing for the 16S rRNA (V3-V4 region) of tick bacterial microbiota and metagenomic analysis were established. The analysis of the bacterial diversity reveals that H. marginatum and H. excavatum have greater diversity than H. scupense. Furthermore, microbial diversity and composition vary according to the tick’s life stage and sex in the specific case of H. scupense. The endosymbionts Francisella, Midichloria mitochondrii, and Rickettsia were shown to be the most prevalent in Hyalomma spp. Rickettsia, Francisella, Ehrlichia, and Erwinia are the most common zoonotic bacteria found in Hyalomma ticks. Accordingly, Hyalomma ticks could represent potential vectors for these zoonotic bacterial agents. |
| format | Journal Article |
| id | CGSpace126568 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Public Library of Science (PLOS ONE) |
| publisherStr | Public Library of Science (PLOS ONE) |
| record_format | dspace |
| spelling | CGSpace1265682026-01-22T02:01:08Z First insights into the microbiome of Tunisian Hyalomma ticks gained through nextgeneration sequencing with a special focus on H. scupense Ben Yedem, Hayet Lekired, Abdelmalek Mhadhbi, Moez Dhibi, Mokhtar Romdhane, Rihab Chaari, Soufiene Rekik, Mourad Ouzari, Hadda-Imene Hajji, Tarek Darghouth, Mohamed Aziz food security health livestock nutrition species diversity pathogens ticks goal 3 good health and well-being francisella rickettsia microbiome Ticks are one of the most important vectors of several pathogens affecting humans and animals. In addition to pathogens, ticks carry diverse microbiota of symbiotic and commensal microorganisms. In this study, we have investigated the first Tunisian insight into the microbial composition of the most dominant Hyalomma species infesting Tunisian cattle and explored the relative contribution of tick sex, life stage, and species to the diversity, richness and bacterial species of tick microbiome. In this regard, next generation sequencing for the 16S rRNA (V3-V4 region) of tick bacterial microbiota and metagenomic analysis were established. The analysis of the bacterial diversity reveals that H. marginatum and H. excavatum have greater diversity than H. scupense. Furthermore, microbial diversity and composition vary according to the tick’s life stage and sex in the specific case of H. scupense. The endosymbionts Francisella, Midichloria mitochondrii, and Rickettsia were shown to be the most prevalent in Hyalomma spp. Rickettsia, Francisella, Ehrlichia, and Erwinia are the most common zoonotic bacteria found in Hyalomma ticks. Accordingly, Hyalomma ticks could represent potential vectors for these zoonotic bacterial agents. 2023-01-04T19:51:57Z 2023-01-04T19:51:57Z Journal Article https://hdl.handle.net/10568/126568 en Open Access application/pdf Public Library of Science (PLOS ONE) Hayet Ben Yedem, Abdelmalek Lekired, Moez Mhadhbi, Mokhtar Dhibi, Rihab Romdhane, Soufiene Chaari, Mourad Rekik, Hadda-Imene Ouzari, Tarek Hajji, Mohamed Aziz Darghouth. (19/5/2022). First insights into the microbiome of Tunisian Hyalomma ticks gained through nextgeneration sequencing with a special focus on H. scupense. PLoS ONE, 17 (5). |
| spellingShingle | food security health livestock nutrition species diversity pathogens ticks goal 3 good health and well-being francisella rickettsia microbiome Ben Yedem, Hayet Lekired, Abdelmalek Mhadhbi, Moez Dhibi, Mokhtar Romdhane, Rihab Chaari, Soufiene Rekik, Mourad Ouzari, Hadda-Imene Hajji, Tarek Darghouth, Mohamed Aziz First insights into the microbiome of Tunisian Hyalomma ticks gained through nextgeneration sequencing with a special focus on H. scupense |
| title | First insights into the microbiome of Tunisian Hyalomma ticks gained through nextgeneration sequencing with a special focus on H. scupense |
| title_full | First insights into the microbiome of Tunisian Hyalomma ticks gained through nextgeneration sequencing with a special focus on H. scupense |
| title_fullStr | First insights into the microbiome of Tunisian Hyalomma ticks gained through nextgeneration sequencing with a special focus on H. scupense |
| title_full_unstemmed | First insights into the microbiome of Tunisian Hyalomma ticks gained through nextgeneration sequencing with a special focus on H. scupense |
| title_short | First insights into the microbiome of Tunisian Hyalomma ticks gained through nextgeneration sequencing with a special focus on H. scupense |
| title_sort | first insights into the microbiome of tunisian hyalomma ticks gained through nextgeneration sequencing with a special focus on h scupense |
| topic | food security health livestock nutrition species diversity pathogens ticks goal 3 good health and well-being francisella rickettsia microbiome |
| url | https://hdl.handle.net/10568/126568 |
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