Genetic Diversity, Population Structure and Subset Development in a Sesbania sesban Collection
Sesbania sesban (L.) Merr. is a multipurpose legume tree grown primarily for fodder and forage in the tropical and subtropical world. In this study, the Sesbania sesban collection maintained in the International Livestock Research Institute (ILRI) forage Genebank was studied using genome-wide marker...
| Autores principales: | , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
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MDPI
2022
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/126549 |
| _version_ | 1855539006381490176 |
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| author | Negawo, Alemayehu T. Akinmade, Habib Olumide Muktar, Meki S. Habte, Ermias Assefa, Yilikal Muchugi, Alice Sartie, Alieu Jones, Christopher S. |
| author_browse | Akinmade, Habib Olumide Assefa, Yilikal Habte, Ermias Jones, Christopher S. Muchugi, Alice Muktar, Meki S. Negawo, Alemayehu T. Sartie, Alieu |
| author_facet | Negawo, Alemayehu T. Akinmade, Habib Olumide Muktar, Meki S. Habte, Ermias Assefa, Yilikal Muchugi, Alice Sartie, Alieu Jones, Christopher S. |
| author_sort | Negawo, Alemayehu T. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Sesbania sesban (L.) Merr. is a multipurpose legume tree grown primarily for fodder and forage in the tropical and subtropical world. In this study, the Sesbania sesban collection maintained in the International Livestock Research Institute (ILRI) forage Genebank was studied using genome-wide markers generated on the DArTseq platform. Genotyping produced 84,673 and 60,626 SNP and SilicoDArT markers with a mean polymorphic information content of 0.153 and 0.123, respectively. From the generated markers, 7587 and 15,031 highly informative SNP and SilicoDArT markers, respectively, were filtered and used for genetic diversity analysis and subset development. Analysis of molecular variance (AMOVA) revealed higher variability ‘within’ (52.73% for SNP markers and 67.36% for SilicoDArT markers) than ‘between’ accessions. Hierarchical cluster analysis showed the presence of four main clusters in the collection. Mantel correlation analysis showed a lack of relationship between genetic variation of the germplasm and their geographical origin. A representative subset of 34 accessions containing germplasm from diverse origins and agro-ecologies was developed using SNP markers. The genetic diversity information generated in this study could be used for marker-assisted screening for stress tolerance, gap analysis and identification and acquisition of new distinct genotype(s) to broaden the genetic basis of the collection for future improvement programs to develop high-yielding, stress-tolerant varieties for enhancing food and environmental security in crop–livestock-based production systems. |
| format | Journal Article |
| id | CGSpace126549 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | CGSpace1265492025-12-08T10:29:22Z Genetic Diversity, Population Structure and Subset Development in a Sesbania sesban Collection Negawo, Alemayehu T. Akinmade, Habib Olumide Muktar, Meki S. Habte, Ermias Assefa, Yilikal Muchugi, Alice Sartie, Alieu Jones, Christopher S. dartseq genetic diversity sesbania sesban subset Sesbania sesban (L.) Merr. is a multipurpose legume tree grown primarily for fodder and forage in the tropical and subtropical world. In this study, the Sesbania sesban collection maintained in the International Livestock Research Institute (ILRI) forage Genebank was studied using genome-wide markers generated on the DArTseq platform. Genotyping produced 84,673 and 60,626 SNP and SilicoDArT markers with a mean polymorphic information content of 0.153 and 0.123, respectively. From the generated markers, 7587 and 15,031 highly informative SNP and SilicoDArT markers, respectively, were filtered and used for genetic diversity analysis and subset development. Analysis of molecular variance (AMOVA) revealed higher variability ‘within’ (52.73% for SNP markers and 67.36% for SilicoDArT markers) than ‘between’ accessions. Hierarchical cluster analysis showed the presence of four main clusters in the collection. Mantel correlation analysis showed a lack of relationship between genetic variation of the germplasm and their geographical origin. A representative subset of 34 accessions containing germplasm from diverse origins and agro-ecologies was developed using SNP markers. The genetic diversity information generated in this study could be used for marker-assisted screening for stress tolerance, gap analysis and identification and acquisition of new distinct genotype(s) to broaden the genetic basis of the collection for future improvement programs to develop high-yielding, stress-tolerant varieties for enhancing food and environmental security in crop–livestock-based production systems. 2022-12-20 2023-01-04T12:38:08Z 2023-01-04T12:38:08Z Journal Article https://hdl.handle.net/10568/126549 en Open Access MDPI Negawo, A.T., Akinmade, H.O., Muktar, M.S.; Habte, E., Assefa, Y., Muchugi, A., Sartie, A.M. and Jones, C.S. 2023. Genetic Diversity, Population Structure and Subset Development in a Sesbania sesban Collection. Plants 2023, 12, 13 |
| spellingShingle | dartseq genetic diversity sesbania sesban subset Negawo, Alemayehu T. Akinmade, Habib Olumide Muktar, Meki S. Habte, Ermias Assefa, Yilikal Muchugi, Alice Sartie, Alieu Jones, Christopher S. Genetic Diversity, Population Structure and Subset Development in a Sesbania sesban Collection |
| title | Genetic Diversity, Population Structure and Subset Development in a Sesbania sesban Collection |
| title_full | Genetic Diversity, Population Structure and Subset Development in a Sesbania sesban Collection |
| title_fullStr | Genetic Diversity, Population Structure and Subset Development in a Sesbania sesban Collection |
| title_full_unstemmed | Genetic Diversity, Population Structure and Subset Development in a Sesbania sesban Collection |
| title_short | Genetic Diversity, Population Structure and Subset Development in a Sesbania sesban Collection |
| title_sort | genetic diversity population structure and subset development in a sesbania sesban collection |
| topic | dartseq genetic diversity sesbania sesban subset |
| url | https://hdl.handle.net/10568/126549 |
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