Genome-wide analysis of Circular RNAs reveals circCHRNG regulates sheep myoblast proliferation via miR-133/SRF and MEF2A Axis
As relatively new members of the non-coding RNA family, circRNAs play important roles in a variety of biological processes. However, the temporal expression pattern and the function of circRNAs during sheep skeletal muscle development remains unclear. This study aimed to identify circRNAs related to...
| Autores principales: | , , , , , |
|---|---|
| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
MDPI
2022
|
| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/126542 |
| _version_ | 1855541023816548352 |
|---|---|
| author | Yue Liu Qian Chen Jingjing Bao Yabin Pu Han Jianlin Huijing Zhao |
| author_browse | Han Jianlin Huijing Zhao Jingjing Bao Qian Chen Yabin Pu Yue Liu |
| author_facet | Yue Liu Qian Chen Jingjing Bao Yabin Pu Han Jianlin Huijing Zhao |
| author_sort | Yue Liu |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | As relatively new members of the non-coding RNA family, circRNAs play important roles in a variety of biological processes. However, the temporal expression pattern and the function of circRNAs during sheep skeletal muscle development remains unclear. This study aimed to identify circRNAs related to sheep skeletal muscle development and explore their roles in myoblast proliferation. The circRNA expression profiles of longissimus dorsi of sheep from F90, L30, and A3Y were obtained by the RNA-seq method. The function and mechanisms of the novel circCHRNG in muscle satellite cell proliferation were explored using CCK-8 assay, Western blot, qPCR, and dual-luciferase reporter assay. We identified 12,375 circRNAs, including 476, 133, and 233 DEcircRNAs found among three comparative groups. KEGG results showed that DEcircRNAs were enriched in muscle contraction, the regulation of cell proliferation, and the AMPK, insulin, and PI3K-Akt signaling pathways. Notably, a novel circRNA, termed circRNA CHRNG, acts as a miR-133 sponge to promote skeletal muscle satellite cell proliferation. Our study provides a systematic description of circRNAs of ovine skeletal muscle across fetal, lamb, and adult stages. GO and KEGG analyses showed that DEcircRNAs were enriched in multiple pathways associated with muscle development, such as the PI3K-Akt and AMPK signaling pathways. In addition, we propose that circCHRNG acts as a miR-133 sponge to upregulate the expression levels of SRF and MEF2A, thereby promoting myoblast proliferation. |
| format | Journal Article |
| id | CGSpace126542 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | CGSpace1265422025-12-08T10:29:22Z Genome-wide analysis of Circular RNAs reveals circCHRNG regulates sheep myoblast proliferation via miR-133/SRF and MEF2A Axis Yue Liu Qian Chen Jingjing Bao Yabin Pu Han Jianlin Huijing Zhao animal breeding genetics sheep small ruminants As relatively new members of the non-coding RNA family, circRNAs play important roles in a variety of biological processes. However, the temporal expression pattern and the function of circRNAs during sheep skeletal muscle development remains unclear. This study aimed to identify circRNAs related to sheep skeletal muscle development and explore their roles in myoblast proliferation. The circRNA expression profiles of longissimus dorsi of sheep from F90, L30, and A3Y were obtained by the RNA-seq method. The function and mechanisms of the novel circCHRNG in muscle satellite cell proliferation were explored using CCK-8 assay, Western blot, qPCR, and dual-luciferase reporter assay. We identified 12,375 circRNAs, including 476, 133, and 233 DEcircRNAs found among three comparative groups. KEGG results showed that DEcircRNAs were enriched in muscle contraction, the regulation of cell proliferation, and the AMPK, insulin, and PI3K-Akt signaling pathways. Notably, a novel circRNA, termed circRNA CHRNG, acts as a miR-133 sponge to promote skeletal muscle satellite cell proliferation. Our study provides a systematic description of circRNAs of ovine skeletal muscle across fetal, lamb, and adult stages. GO and KEGG analyses showed that DEcircRNAs were enriched in multiple pathways associated with muscle development, such as the PI3K-Akt and AMPK signaling pathways. In addition, we propose that circCHRNG acts as a miR-133 sponge to upregulate the expression levels of SRF and MEF2A, thereby promoting myoblast proliferation. 2022-12-16 2023-01-04T10:21:51Z 2023-01-04T10:21:51Z Journal Article https://hdl.handle.net/10568/126542 en Open Access MDPI Yue Liu, Qian Chen, Jingjing Bao, Yabin Pu, Jianlin Han and Huijing Zhao. 2022. Genome-wide analysis of Circular RNAs reveals circCHRNG regulates sheep myoblast proliferation via miR-133/SRF and MEF2A Axis. International Journal of Molecular Sciences 23(24):16065. |
| spellingShingle | animal breeding genetics sheep small ruminants Yue Liu Qian Chen Jingjing Bao Yabin Pu Han Jianlin Huijing Zhao Genome-wide analysis of Circular RNAs reveals circCHRNG regulates sheep myoblast proliferation via miR-133/SRF and MEF2A Axis |
| title | Genome-wide analysis of Circular RNAs reveals circCHRNG regulates sheep myoblast proliferation via miR-133/SRF and MEF2A Axis |
| title_full | Genome-wide analysis of Circular RNAs reveals circCHRNG regulates sheep myoblast proliferation via miR-133/SRF and MEF2A Axis |
| title_fullStr | Genome-wide analysis of Circular RNAs reveals circCHRNG regulates sheep myoblast proliferation via miR-133/SRF and MEF2A Axis |
| title_full_unstemmed | Genome-wide analysis of Circular RNAs reveals circCHRNG regulates sheep myoblast proliferation via miR-133/SRF and MEF2A Axis |
| title_short | Genome-wide analysis of Circular RNAs reveals circCHRNG regulates sheep myoblast proliferation via miR-133/SRF and MEF2A Axis |
| title_sort | genome wide analysis of circular rnas reveals circchrng regulates sheep myoblast proliferation via mir 133 srf and mef2a axis |
| topic | animal breeding genetics sheep small ruminants |
| url | https://hdl.handle.net/10568/126542 |
| work_keys_str_mv | AT yueliu genomewideanalysisofcircularrnasrevealscircchrngregulatessheepmyoblastproliferationviamir133srfandmef2aaxis AT qianchen genomewideanalysisofcircularrnasrevealscircchrngregulatessheepmyoblastproliferationviamir133srfandmef2aaxis AT jingjingbao genomewideanalysisofcircularrnasrevealscircchrngregulatessheepmyoblastproliferationviamir133srfandmef2aaxis AT yabinpu genomewideanalysisofcircularrnasrevealscircchrngregulatessheepmyoblastproliferationviamir133srfandmef2aaxis AT hanjianlin genomewideanalysisofcircularrnasrevealscircchrngregulatessheepmyoblastproliferationviamir133srfandmef2aaxis AT huijingzhao genomewideanalysisofcircularrnasrevealscircchrngregulatessheepmyoblastproliferationviamir133srfandmef2aaxis |