Resistance response of drought and heat tolerant spring wheat lines against the cereal cyst nematode, heterodera filipjevi
Spot blotch caused by Bipolaris sorokiniana is a destructive disease of wheat worldwide. This study investigated the aggressiveness of B. sorokiniana isolates from different wheat-growing areas of Bolu province in Turkey on the cultivar Seri-82. Host susceptibility of 55 wheat cultivars was evaluate...
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| Format: | Journal Article |
| Language: | Inglés |
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Ankara University Faculty of Agriculture
2022
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| Online Access: | https://hdl.handle.net/10568/126528 |
| _version_ | 1855539732801388544 |
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| author | Atiya, Osameh Dababat, Abdelfattah A. Erginbas-Orakci, Gul Singh, Sukhwinder Toktay, Halil |
| author_browse | Atiya, Osameh Dababat, Abdelfattah A. Erginbas-Orakci, Gul Singh, Sukhwinder Toktay, Halil |
| author_facet | Atiya, Osameh Dababat, Abdelfattah A. Erginbas-Orakci, Gul Singh, Sukhwinder Toktay, Halil |
| author_sort | Atiya, Osameh |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Spot blotch caused by Bipolaris sorokiniana is a destructive disease of wheat worldwide. This study investigated the aggressiveness of B. sorokiniana isolates from different wheat-growing areas of Bolu province in Turkey on the cultivar Seri-82. Host susceptibility of 55 wheat cultivars was evaluated against the most aggressive isolate. Our results indicated that the cultivars Anafarta and Koç-2015 were the most resistant. A specific and sensitive qPCR assay was developed for detecting the pathogen in plant tissues and evaluating wheat plants with different resistance levels. Three primer sets, BsGAPDHF/BsGAPDHR, BsITSF/BsITSR, and BsSSUF/BsSSUR, were designed based on glyceraldehyde-3-phosphate dehydrogenase, internal transcribed spacers, and 18S rRNA loci of B. sorokiniana with detection limits of 1, 0.1, and 0.1 pg of pathogen DNA, respectively. The qPCR assay was highly sensitive and did not amplify DNA from the other closely related fungal species and host plants. The protocol differentiated wheat plants with varying degrees of resistance. The assay developed a useful tool for the quantification of the pathogen in the early stages of infection and may provide a significant contribution to a more efficient selection of wheat genotypes in breeding studies. In the present study, expression levels of PR proteins, phenylalanine ammonia-lyase, catalase, ascorbate peroxidase, and superoxide dismutase enzymes were upregulated in Anafarta (resistant) and Nenehatun (susceptible) cultivars at different post-infection time points, but more induced in the susceptible cultivar. The results showed considerable variation in the expression levels and timing of defense genes in both cultivars. |
| format | Journal Article |
| id | CGSpace126528 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Ankara University Faculty of Agriculture |
| publisherStr | Ankara University Faculty of Agriculture |
| record_format | dspace |
| spelling | CGSpace1265282025-11-06T13:09:26Z Resistance response of drought and heat tolerant spring wheat lines against the cereal cyst nematode, heterodera filipjevi Atiya, Osameh Dababat, Abdelfattah A. Erginbas-Orakci, Gul Singh, Sukhwinder Toktay, Halil biotic stress breeding cereal crops plant nematodes spring wheat drought resistance Spot blotch caused by Bipolaris sorokiniana is a destructive disease of wheat worldwide. This study investigated the aggressiveness of B. sorokiniana isolates from different wheat-growing areas of Bolu province in Turkey on the cultivar Seri-82. Host susceptibility of 55 wheat cultivars was evaluated against the most aggressive isolate. Our results indicated that the cultivars Anafarta and Koç-2015 were the most resistant. A specific and sensitive qPCR assay was developed for detecting the pathogen in plant tissues and evaluating wheat plants with different resistance levels. Three primer sets, BsGAPDHF/BsGAPDHR, BsITSF/BsITSR, and BsSSUF/BsSSUR, were designed based on glyceraldehyde-3-phosphate dehydrogenase, internal transcribed spacers, and 18S rRNA loci of B. sorokiniana with detection limits of 1, 0.1, and 0.1 pg of pathogen DNA, respectively. The qPCR assay was highly sensitive and did not amplify DNA from the other closely related fungal species and host plants. The protocol differentiated wheat plants with varying degrees of resistance. The assay developed a useful tool for the quantification of the pathogen in the early stages of infection and may provide a significant contribution to a more efficient selection of wheat genotypes in breeding studies. In the present study, expression levels of PR proteins, phenylalanine ammonia-lyase, catalase, ascorbate peroxidase, and superoxide dismutase enzymes were upregulated in Anafarta (resistant) and Nenehatun (susceptible) cultivars at different post-infection time points, but more induced in the susceptible cultivar. The results showed considerable variation in the expression levels and timing of defense genes in both cultivars. 2022-04-25 2023-01-04T08:03:45Z 2023-01-04T08:03:45Z Journal Article https://hdl.handle.net/10568/126528 en Open Access application/pdf Ankara University Faculty of Agriculture ATİYA, O., DABABAT, A., ERGİNBAS-ORAKCİ, G., SİNGH, S., & TOKTAY, H. (2021). Resistance Response of Drought and Heat Tolerant Spring Wheat Lines against the Cereal Cyst Nematode Heterodera filipjevi. Tarım Bilimleri Dergisi. https://doi.org/10.15832/ankutbd.818047 |
| spellingShingle | biotic stress breeding cereal crops plant nematodes spring wheat drought resistance Atiya, Osameh Dababat, Abdelfattah A. Erginbas-Orakci, Gul Singh, Sukhwinder Toktay, Halil Resistance response of drought and heat tolerant spring wheat lines against the cereal cyst nematode, heterodera filipjevi |
| title | Resistance response of drought and heat tolerant spring wheat lines against the cereal cyst nematode, heterodera filipjevi |
| title_full | Resistance response of drought and heat tolerant spring wheat lines against the cereal cyst nematode, heterodera filipjevi |
| title_fullStr | Resistance response of drought and heat tolerant spring wheat lines against the cereal cyst nematode, heterodera filipjevi |
| title_full_unstemmed | Resistance response of drought and heat tolerant spring wheat lines against the cereal cyst nematode, heterodera filipjevi |
| title_short | Resistance response of drought and heat tolerant spring wheat lines against the cereal cyst nematode, heterodera filipjevi |
| title_sort | resistance response of drought and heat tolerant spring wheat lines against the cereal cyst nematode heterodera filipjevi |
| topic | biotic stress breeding cereal crops plant nematodes spring wheat drought resistance |
| url | https://hdl.handle.net/10568/126528 |
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