MicroRNA-181a Regulates the Proliferation and Differentiation of Hu Sheep Skeletal Muscle Satellite Cells and Targets the YAP1 Gene
MicroRNA (miRNA) is of great importance to muscle growth and development, including the regulation of the proliferation and differentiation of skeletal muscle satellite cells (SMSCs). In our research group’s previous study, we found that miR-181a is differentially expressed in the longissimus dorsi...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
MDPI
2023
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/126521 |
| _version_ | 1855541639615873024 |
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| author | He, Mingliang Zhang, Weibo Wang, Shan Ge, Ling Cao, Xiukai Wang, Shanhe Yuan, Zehu Lv, Xiaoyang Getachew, Tesfaye Mwacharo, Joram Haile, Aynalem Sun, Wei |
| author_browse | Cao, Xiukai Ge, Ling Getachew, Tesfaye Haile, Aynalem He, Mingliang Lv, Xiaoyang Mwacharo, Joram Sun, Wei Wang, Shan Wang, Shanhe Yuan, Zehu Zhang, Weibo |
| author_facet | He, Mingliang Zhang, Weibo Wang, Shan Ge, Ling Cao, Xiukai Wang, Shanhe Yuan, Zehu Lv, Xiaoyang Getachew, Tesfaye Mwacharo, Joram Haile, Aynalem Sun, Wei |
| author_sort | He, Mingliang |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | MicroRNA (miRNA) is of great importance to muscle growth and development, including
the regulation of the proliferation and differentiation of skeletal muscle satellite cells (SMSCs). In our
research group’s previous study, we found that miR-181a is differentially expressed in the longissimus
dorsi muscle of Hu sheep at different stages. We speculated that miR-181a may participate in the
growth and development process of Hu sheep. To understand the mechanism of miR-181a regulating
the growth and development of Hu sheep skeletal muscle, we extracted skeletal muscle satellite
cells from the longissimus dorsi muscle of 3-month-old Hu sheep fetuses and performed a series of
experiments. Our results showed that miR-181a suppressed SMSCs’ proliferation using QRT-PCR,
Western blot, CCK-8, EDU, and Flow cytometry cycle tests. In addition, QRT-PCR, Western blot,
and immunofluorescence indicated that miR-181a facilitated the differentiation of SMSCs. Then, we
used dual-luciferase reporter gene detection, QRT-PCR, and Western blot to find that the Yes1-related
transcription regulator (YAP1) is the target gene of miR-181a. Our study supplies a research basis for
understanding the regulation mechanism of miR-181a on the growth of Hu sheep skeletal muscle. |
| format | Journal Article |
| id | CGSpace126521 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | CGSpace1265212026-01-14T02:17:02Z MicroRNA-181a Regulates the Proliferation and Differentiation of Hu Sheep Skeletal Muscle Satellite Cells and Targets the YAP1 Gene He, Mingliang Zhang, Weibo Wang, Shan Ge, Ling Cao, Xiukai Wang, Shanhe Yuan, Zehu Lv, Xiaoyang Getachew, Tesfaye Mwacharo, Joram Haile, Aynalem Sun, Wei goal 1 no poverty sheep differentiation hu sheep poverty reduction, livelihoods and jobs mir-181a yap1 proliferation satellite cell MicroRNA (miRNA) is of great importance to muscle growth and development, including the regulation of the proliferation and differentiation of skeletal muscle satellite cells (SMSCs). In our research group’s previous study, we found that miR-181a is differentially expressed in the longissimus dorsi muscle of Hu sheep at different stages. We speculated that miR-181a may participate in the growth and development process of Hu sheep. To understand the mechanism of miR-181a regulating the growth and development of Hu sheep skeletal muscle, we extracted skeletal muscle satellite cells from the longissimus dorsi muscle of 3-month-old Hu sheep fetuses and performed a series of experiments. Our results showed that miR-181a suppressed SMSCs’ proliferation using QRT-PCR, Western blot, CCK-8, EDU, and Flow cytometry cycle tests. In addition, QRT-PCR, Western blot, and immunofluorescence indicated that miR-181a facilitated the differentiation of SMSCs. Then, we used dual-luciferase reporter gene detection, QRT-PCR, and Western blot to find that the Yes1-related transcription regulator (YAP1) is the target gene of miR-181a. Our study supplies a research basis for understanding the regulation mechanism of miR-181a on the growth of Hu sheep skeletal muscle. 2023-01-03T21:53:35Z 2023-01-03T21:53:35Z Journal Article https://hdl.handle.net/10568/126521 en Open Access application/pdf MDPI Mingliang He, Weibo Zhang, Shan Wang, Ling Ge, Xiukai Cao, Shanhe Wang, Zehu Yuan, Xiaoyang Lv, Tesfaye Getachew, Joram Mwacharo, Aynalem Haile, Wei Sun. (16/3/2022). MicroRNA-181a Regulates the Proliferation and Differentiation of Hu Sheep Skeletal Muscle Satellite Cells and Targets the YAP1 Gene. Genes, 13 (3). |
| spellingShingle | goal 1 no poverty sheep differentiation hu sheep poverty reduction, livelihoods and jobs mir-181a yap1 proliferation satellite cell He, Mingliang Zhang, Weibo Wang, Shan Ge, Ling Cao, Xiukai Wang, Shanhe Yuan, Zehu Lv, Xiaoyang Getachew, Tesfaye Mwacharo, Joram Haile, Aynalem Sun, Wei MicroRNA-181a Regulates the Proliferation and Differentiation of Hu Sheep Skeletal Muscle Satellite Cells and Targets the YAP1 Gene |
| title | MicroRNA-181a Regulates the Proliferation and Differentiation of Hu Sheep Skeletal Muscle Satellite Cells and Targets the YAP1 Gene |
| title_full | MicroRNA-181a Regulates the Proliferation and Differentiation of Hu Sheep Skeletal Muscle Satellite Cells and Targets the YAP1 Gene |
| title_fullStr | MicroRNA-181a Regulates the Proliferation and Differentiation of Hu Sheep Skeletal Muscle Satellite Cells and Targets the YAP1 Gene |
| title_full_unstemmed | MicroRNA-181a Regulates the Proliferation and Differentiation of Hu Sheep Skeletal Muscle Satellite Cells and Targets the YAP1 Gene |
| title_short | MicroRNA-181a Regulates the Proliferation and Differentiation of Hu Sheep Skeletal Muscle Satellite Cells and Targets the YAP1 Gene |
| title_sort | microrna 181a regulates the proliferation and differentiation of hu sheep skeletal muscle satellite cells and targets the yap1 gene |
| topic | goal 1 no poverty sheep differentiation hu sheep poverty reduction, livelihoods and jobs mir-181a yap1 proliferation satellite cell |
| url | https://hdl.handle.net/10568/126521 |
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