Marker development for quality protein maize breeding and an interaction study between Opaque-2 and Ask2 genes
Quality Protein Maize (QPM) kernels contain twice the amounts of lysine and tryptophan compared to normal corn kernels. Although the opaque-2 (o2) mutation is the underlying cause of this beneficial change, other genes such as Aspartate kinase-2 (Ask2) affect the amino acid content in the endosperm...
| Main Authors: | , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Sociedad Mexicana de Fitogenetica A.C
2022
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/126507 |
| _version_ | 1855531460559110144 |
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| author | Hernández Rodríguez, Martha Babu, Raman Skinner, Debra J. Palacios Rojas, Natalia Martins, Marina C.M. García Zavala, J. Jesús Lobato Ortiz, Ricardo Santacruz Varela, Amalio Castillo-González, Fernando Yunbi Xu Boddupalli, P.M. |
| author_browse | Babu, Raman Boddupalli, P.M. Castillo-González, Fernando García Zavala, J. Jesús Hernández Rodríguez, Martha Lobato Ortiz, Ricardo Martins, Marina C.M. Palacios Rojas, Natalia Santacruz Varela, Amalio Skinner, Debra J. Yunbi Xu |
| author_facet | Hernández Rodríguez, Martha Babu, Raman Skinner, Debra J. Palacios Rojas, Natalia Martins, Marina C.M. García Zavala, J. Jesús Lobato Ortiz, Ricardo Santacruz Varela, Amalio Castillo-González, Fernando Yunbi Xu Boddupalli, P.M. |
| author_sort | Hernández Rodríguez, Martha |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Quality Protein Maize (QPM) kernels contain twice the amounts of lysine and tryptophan compared to normal corn kernels. Although the opaque-2 (o2) mutation is the underlying cause of this beneficial change, other genes such as Aspartate kinase-2 (Ask2) affect the amino acid content in the endosperm to a lesser degree. To date, reports on the interaction between both loci are scarce and there are no high-throughput assays for the identification of the alleles of these genes. The objectives of this research were: 1) to study the interaction between the o2 and Ask2 genes with respect to the accumulation of amino acids in the endosperm in an F2 population, 2) to identify conserved SNPs into the o2 gene that can be used as markers, 3) to estimate the frequency of an SNP of Ask2 associated with the accumulation of lysine in the endosperm in CIMMYT germplasm, and 4) to develop high-throughput marker assays for these SNPs. The interaction study showed a preponderant effect of o2 on the accumulation of 11 amino acids (P ≤ 0.01). Ask2 appeared only to act with o2 to enhance marginally lysine, histidine and methionine levels in the double recessive homozygotes. Sequencing of amplicons at the o2 locus led to the identification of an SNP in exon 1 that discriminated all QPM (C) genotypes from non-QPM (T) genotypes. Validation of this SNP through KASP™ assays indicated that it was 92 % assertive in differentiating the o2 genotypes. In contrast, the frequency of the Ask2 SNP in CIMMYT QPM germplasm was low; however, an SSCP marker developed using this SNP detected five variants indicating that other unknown base changes may confer positive lysine-increasing responses. These markers could aid the marker-assisted selection of QPM cultivars. |
| format | Journal Article |
| id | CGSpace126507 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Sociedad Mexicana de Fitogenetica A.C |
| publisherStr | Sociedad Mexicana de Fitogenetica A.C |
| record_format | dspace |
| spelling | CGSpace1265072025-11-06T13:07:14Z Marker development for quality protein maize breeding and an interaction study between Opaque-2 and Ask2 genes Hernández Rodríguez, Martha Babu, Raman Skinner, Debra J. Palacios Rojas, Natalia Martins, Marina C.M. García Zavala, J. Jesús Lobato Ortiz, Ricardo Santacruz Varela, Amalio Castillo-González, Fernando Yunbi Xu Boddupalli, P.M. zea mays marker-assisted selection single nucleotide polymorphism protein quality Quality Protein Maize (QPM) kernels contain twice the amounts of lysine and tryptophan compared to normal corn kernels. Although the opaque-2 (o2) mutation is the underlying cause of this beneficial change, other genes such as Aspartate kinase-2 (Ask2) affect the amino acid content in the endosperm to a lesser degree. To date, reports on the interaction between both loci are scarce and there are no high-throughput assays for the identification of the alleles of these genes. The objectives of this research were: 1) to study the interaction between the o2 and Ask2 genes with respect to the accumulation of amino acids in the endosperm in an F2 population, 2) to identify conserved SNPs into the o2 gene that can be used as markers, 3) to estimate the frequency of an SNP of Ask2 associated with the accumulation of lysine in the endosperm in CIMMYT germplasm, and 4) to develop high-throughput marker assays for these SNPs. The interaction study showed a preponderant effect of o2 on the accumulation of 11 amino acids (P ≤ 0.01). Ask2 appeared only to act with o2 to enhance marginally lysine, histidine and methionine levels in the double recessive homozygotes. Sequencing of amplicons at the o2 locus led to the identification of an SNP in exon 1 that discriminated all QPM (C) genotypes from non-QPM (T) genotypes. Validation of this SNP through KASP™ assays indicated that it was 92 % assertive in differentiating the o2 genotypes. In contrast, the frequency of the Ask2 SNP in CIMMYT QPM germplasm was low; however, an SSCP marker developed using this SNP detected five variants indicating that other unknown base changes may confer positive lysine-increasing responses. These markers could aid the marker-assisted selection of QPM cultivars. 2022-05-30 2023-01-03T14:32:35Z 2023-01-03T14:32:35Z Journal Article https://hdl.handle.net/10568/126507 en Open Access application/pdf Sociedad Mexicana de Fitogenetica A.C Hernández-Rodríguez, M., Babu, R., Skinner, D. J., Palacios-Rojas, N., Martins, M. C. M., García-Zavala, J. J., Lobato-Ortiz, R., Santacruz-Varela, A., Castillo-González, F., Xu, Y., & Prasanna, B. M. (2022). DESARROLLO DE MARCADORES PARA MEJORAMIENTO DE MAÍZ CON PROTEÍNA DE CALIDAD Y ESTUDIO DE INTERACCIÓN ENTRE LOS GENES Opaco-2 Y Ask2. Revista Fitotecnia Mexicana, 45(3), 293. https://doi.org/10.35196/rfm.2022.3.293 |
| spellingShingle | zea mays marker-assisted selection single nucleotide polymorphism protein quality Hernández Rodríguez, Martha Babu, Raman Skinner, Debra J. Palacios Rojas, Natalia Martins, Marina C.M. García Zavala, J. Jesús Lobato Ortiz, Ricardo Santacruz Varela, Amalio Castillo-González, Fernando Yunbi Xu Boddupalli, P.M. Marker development for quality protein maize breeding and an interaction study between Opaque-2 and Ask2 genes |
| title | Marker development for quality protein maize breeding and an interaction study between Opaque-2 and Ask2 genes |
| title_full | Marker development for quality protein maize breeding and an interaction study between Opaque-2 and Ask2 genes |
| title_fullStr | Marker development for quality protein maize breeding and an interaction study between Opaque-2 and Ask2 genes |
| title_full_unstemmed | Marker development for quality protein maize breeding and an interaction study between Opaque-2 and Ask2 genes |
| title_short | Marker development for quality protein maize breeding and an interaction study between Opaque-2 and Ask2 genes |
| title_sort | marker development for quality protein maize breeding and an interaction study between opaque 2 and ask2 genes |
| topic | zea mays marker-assisted selection single nucleotide polymorphism protein quality |
| url | https://hdl.handle.net/10568/126507 |
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