Genome-wide association study for resistance to tan spot in synthetic hexaploid wheat

Synthetic hexaploid wheat (SHW) has shown effective resistance to a diversity of diseases and insects, including tan spot, which is caused by Pyrenophora tritici-repentis, being an important foliar disease that can attack all types of wheat and several grasses. In this study, 443 SHW plants were eva...

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Main Authors: Lozano Ramirez, Nerida, Dreisigacker, Susanne, Sansaloni, Carolina P., Xinyao He, Sandoval-Islas, Sergio, Pérez Rodriguez, Paulino, Carballo-Carballo, Aquiles, Nava Díaz, Cristian, Kishii, Masahiro, Singh, Pawan K.
Format: Journal Article
Language:Inglés
Published: MDPI 2022
Subjects:
Online Access:https://hdl.handle.net/10568/126501
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author Lozano Ramirez, Nerida
Dreisigacker, Susanne
Sansaloni, Carolina P.
Xinyao He
Sandoval-Islas, Sergio
Pérez Rodriguez, Paulino
Carballo-Carballo, Aquiles
Nava Díaz, Cristian
Kishii, Masahiro
Singh, Pawan K.
author_browse Carballo-Carballo, Aquiles
Dreisigacker, Susanne
Kishii, Masahiro
Lozano Ramirez, Nerida
Nava Díaz, Cristian
Pérez Rodriguez, Paulino
Sandoval-Islas, Sergio
Sansaloni, Carolina P.
Singh, Pawan K.
Xinyao He
author_facet Lozano Ramirez, Nerida
Dreisigacker, Susanne
Sansaloni, Carolina P.
Xinyao He
Sandoval-Islas, Sergio
Pérez Rodriguez, Paulino
Carballo-Carballo, Aquiles
Nava Díaz, Cristian
Kishii, Masahiro
Singh, Pawan K.
author_sort Lozano Ramirez, Nerida
collection Repository of Agricultural Research Outputs (CGSpace)
description Synthetic hexaploid wheat (SHW) has shown effective resistance to a diversity of diseases and insects, including tan spot, which is caused by Pyrenophora tritici-repentis, being an important foliar disease that can attack all types of wheat and several grasses. In this study, 443 SHW plants were evaluated for their resistance to tan spot under controlled environmental conditions. Additionally, a genome-wide association study was conducted by genotyping all entries with the DArTSeq technology to identify marker-trait associations for tan spot resistance. Of the 443 SHW plants, 233 showed resistant and 183 moderately resistant reactions, and only 27 were moderately susceptible or susceptible to tan spot. Durum wheat (DW) parents of the SHW showed moderately susceptible to susceptible reactions. A total of 30 significant marker-trait associations were found on chromosomes 1B (4 markers), 1D (1 marker), 2A (1 marker), 2D (2 markers), 3A (4 markers), 3D (3 markers), 4B (1 marker), 5A (4 markers), 6A (6 markers), 6B (1 marker) and 7D (3 markers). In-creased resistance in the SHW in comparison to the DW parents, along with the significant association of resistance with the A and B genome, supported the concept of activating epistasis interaction across the three wheat genomes. Candidate genes coding for F-box and cytochrome P450 proteins that play significant roles in biotic stress resistance were identified for the significant markers. The identified resistant SHW lines can be deployed in wheat breeding for tan spot resistance.
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spelling CGSpace1265012025-12-08T10:29:22Z Genome-wide association study for resistance to tan spot in synthetic hexaploid wheat Lozano Ramirez, Nerida Dreisigacker, Susanne Sansaloni, Carolina P. Xinyao He Sandoval-Islas, Sergio Pérez Rodriguez, Paulino Carballo-Carballo, Aquiles Nava Díaz, Cristian Kishii, Masahiro Singh, Pawan K. aegilops hard wheat hexaploidy spots genomes Synthetic hexaploid wheat (SHW) has shown effective resistance to a diversity of diseases and insects, including tan spot, which is caused by Pyrenophora tritici-repentis, being an important foliar disease that can attack all types of wheat and several grasses. In this study, 443 SHW plants were evaluated for their resistance to tan spot under controlled environmental conditions. Additionally, a genome-wide association study was conducted by genotyping all entries with the DArTSeq technology to identify marker-trait associations for tan spot resistance. Of the 443 SHW plants, 233 showed resistant and 183 moderately resistant reactions, and only 27 were moderately susceptible or susceptible to tan spot. Durum wheat (DW) parents of the SHW showed moderately susceptible to susceptible reactions. A total of 30 significant marker-trait associations were found on chromosomes 1B (4 markers), 1D (1 marker), 2A (1 marker), 2D (2 markers), 3A (4 markers), 3D (3 markers), 4B (1 marker), 5A (4 markers), 6A (6 markers), 6B (1 marker) and 7D (3 markers). In-creased resistance in the SHW in comparison to the DW parents, along with the significant association of resistance with the A and B genome, supported the concept of activating epistasis interaction across the three wheat genomes. Candidate genes coding for F-box and cytochrome P450 proteins that play significant roles in biotic stress resistance were identified for the significant markers. The identified resistant SHW lines can be deployed in wheat breeding for tan spot resistance. 2022 2023-01-03T14:13:03Z 2023-01-03T14:13:03Z Journal Article https://hdl.handle.net/10568/126501 en Open Access application/pdf MDPI Lozano-Ramírez, N., Dreisigacker, S., Sansaloni, C. P., He, X., Islas, S. S., Pérez-Rodríguez, P., Carballo, A. C., Nava-Díaz, C., Kishii, M., & Singh, P. K. (2022). Genome-Wide Association Study for Resistance to Tan Spot in Synthetic Hexaploid Wheat. Plants, 11(3), 433. https://doi.org/10.3390/plants11030433
spellingShingle aegilops
hard wheat
hexaploidy
spots
genomes
Lozano Ramirez, Nerida
Dreisigacker, Susanne
Sansaloni, Carolina P.
Xinyao He
Sandoval-Islas, Sergio
Pérez Rodriguez, Paulino
Carballo-Carballo, Aquiles
Nava Díaz, Cristian
Kishii, Masahiro
Singh, Pawan K.
Genome-wide association study for resistance to tan spot in synthetic hexaploid wheat
title Genome-wide association study for resistance to tan spot in synthetic hexaploid wheat
title_full Genome-wide association study for resistance to tan spot in synthetic hexaploid wheat
title_fullStr Genome-wide association study for resistance to tan spot in synthetic hexaploid wheat
title_full_unstemmed Genome-wide association study for resistance to tan spot in synthetic hexaploid wheat
title_short Genome-wide association study for resistance to tan spot in synthetic hexaploid wheat
title_sort genome wide association study for resistance to tan spot in synthetic hexaploid wheat
topic aegilops
hard wheat
hexaploidy
spots
genomes
url https://hdl.handle.net/10568/126501
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