Monitoring of host suitability and defense-related genes in wheat to Bipolaris sorokiniana
Spot blotch caused by Bipolaris sorokiniana is a destructive disease of wheat worldwide. This study investigated the aggressiveness of B. sorokiniana isolates from different wheat-growing areas of Bolu province in Turkey on the cultivar Seri-82. Host susceptibility of 55 wheat cultivars was evaluate...
| Main Authors: | , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
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MDPI
2022
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/126497 |
| _version_ | 1855518454584442880 |
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| author | Alkan, Mehtap Bayraktar, Harun Imren, Mustafa Ozdemir, Fatih Lahlali, Rachid Mokrini, Fouad Paulitz, Timothy C. Dababat, Abdelfattah A. Özer, Göksel |
| author_browse | Alkan, Mehtap Bayraktar, Harun Dababat, Abdelfattah A. Imren, Mustafa Lahlali, Rachid Mokrini, Fouad Ozdemir, Fatih Paulitz, Timothy C. Özer, Göksel |
| author_facet | Alkan, Mehtap Bayraktar, Harun Imren, Mustafa Ozdemir, Fatih Lahlali, Rachid Mokrini, Fouad Paulitz, Timothy C. Dababat, Abdelfattah A. Özer, Göksel |
| author_sort | Alkan, Mehtap |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Spot blotch caused by Bipolaris sorokiniana is a destructive disease of wheat worldwide. This study investigated the aggressiveness of B. sorokiniana isolates from different wheat-growing areas of Bolu province in Turkey on the cultivar Seri-82. Host susceptibility of 55 wheat cultivars was evaluated against the most aggressive isolate. Our results indicated that the cultivars Anafarta and Koç-2015 were the most resistant. A specific and sensitive qPCR assay was developed for detecting the pathogen in plant tissues and evaluating wheat plants with different resistance levels. Three primer sets, BsGAPDHF/BsGAPDHR, BsITSF/BsITSR, and BsSSUF/BsSSUR, were designed based on glyceraldehyde-3-phosphate dehydrogenase, internal transcribed spacers, and 18S rRNA loci of B. sorokiniana with detection limits of 1, 0.1, and 0.1 pg of pathogen DNA, respectively. The qPCR assay was highly sensitive and did not amplify DNA from the other closely related fungal species and host plants. The protocol differentiated wheat plants with varying degrees of resistance. The assay developed a useful tool for the quantification of the pathogen in the early stages of infection and may provide a significant contribution to a more efficient selection of wheat genotypes in breeding studies. In the present study, expression levels of PR proteins, phenylalanine ammonia-lyase, catalase, ascorbate peroxidase, and superoxide dismutase enzymes were upregulated in Anafarta (resistant) and Nenehatun (susceptible) cultivars at different post-infection time points, but more induced in the susceptible cultivar. The results showed considerable variation in the expression levels and timing of defense genes in both cultivars. |
| format | Journal Article |
| id | CGSpace126497 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | CGSpace1264972025-12-08T10:29:22Z Monitoring of host suitability and defense-related genes in wheat to Bipolaris sorokiniana Alkan, Mehtap Bayraktar, Harun Imren, Mustafa Ozdemir, Fatih Lahlali, Rachid Mokrini, Fouad Paulitz, Timothy C. Dababat, Abdelfattah A. Özer, Göksel diseases gene expression pathogens wheat bipolaris sorokiniana Spot blotch caused by Bipolaris sorokiniana is a destructive disease of wheat worldwide. This study investigated the aggressiveness of B. sorokiniana isolates from different wheat-growing areas of Bolu province in Turkey on the cultivar Seri-82. Host susceptibility of 55 wheat cultivars was evaluated against the most aggressive isolate. Our results indicated that the cultivars Anafarta and Koç-2015 were the most resistant. A specific and sensitive qPCR assay was developed for detecting the pathogen in plant tissues and evaluating wheat plants with different resistance levels. Three primer sets, BsGAPDHF/BsGAPDHR, BsITSF/BsITSR, and BsSSUF/BsSSUR, were designed based on glyceraldehyde-3-phosphate dehydrogenase, internal transcribed spacers, and 18S rRNA loci of B. sorokiniana with detection limits of 1, 0.1, and 0.1 pg of pathogen DNA, respectively. The qPCR assay was highly sensitive and did not amplify DNA from the other closely related fungal species and host plants. The protocol differentiated wheat plants with varying degrees of resistance. The assay developed a useful tool for the quantification of the pathogen in the early stages of infection and may provide a significant contribution to a more efficient selection of wheat genotypes in breeding studies. In the present study, expression levels of PR proteins, phenylalanine ammonia-lyase, catalase, ascorbate peroxidase, and superoxide dismutase enzymes were upregulated in Anafarta (resistant) and Nenehatun (susceptible) cultivars at different post-infection time points, but more induced in the susceptible cultivar. The results showed considerable variation in the expression levels and timing of defense genes in both cultivars. 2022 2023-01-03T13:17:36Z 2023-01-03T13:17:36Z Journal Article https://hdl.handle.net/10568/126497 en Open Access application/pdf MDPI Alkan, M., Bayraktar, H., İmren, M., Özdemir, F., Lahlali, R., Mokrini, F., Paulitz, T., Dababat, A. A., & Özer, G. (2022). Monitoring of Host Suitability and Defense-Related Genes in Wheat to Bipolaris sorokiniana. Journal of Fungi, 8(2), 149. https://doi.org/10.3390/jof8020149 |
| spellingShingle | diseases gene expression pathogens wheat bipolaris sorokiniana Alkan, Mehtap Bayraktar, Harun Imren, Mustafa Ozdemir, Fatih Lahlali, Rachid Mokrini, Fouad Paulitz, Timothy C. Dababat, Abdelfattah A. Özer, Göksel Monitoring of host suitability and defense-related genes in wheat to Bipolaris sorokiniana |
| title | Monitoring of host suitability and defense-related genes in wheat to Bipolaris sorokiniana |
| title_full | Monitoring of host suitability and defense-related genes in wheat to Bipolaris sorokiniana |
| title_fullStr | Monitoring of host suitability and defense-related genes in wheat to Bipolaris sorokiniana |
| title_full_unstemmed | Monitoring of host suitability and defense-related genes in wheat to Bipolaris sorokiniana |
| title_short | Monitoring of host suitability and defense-related genes in wheat to Bipolaris sorokiniana |
| title_sort | monitoring of host suitability and defense related genes in wheat to bipolaris sorokiniana |
| topic | diseases gene expression pathogens wheat bipolaris sorokiniana |
| url | https://hdl.handle.net/10568/126497 |
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