Comparison of non-overlapping maize populations of unequal sizes for resistance to maize lethal necrosis

Contrast between marker-assisted backcross (MABC) and doubled haploid (DH) methods in transferring genes for resistance to maize lethal necrosis (MLN) in maize (Zea mays L.) is not well understood. The MLN is caused by co-infection of maize plant by maize chlorotic mottle virus and sugarcane mosaic...

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Autores principales: Awata, Luka A. O., Ifie, Beatrice E., Danquah, Eric, Tongoona, Pangirayi, Mahabaleswara, Suresh L., Gowda, Manje, Marchelo-Dragga, Philip W., Sitonik, Chelang’At, Olsen, Michael, Boddupalli, P.M., Jumbo, McDonald Bright
Formato: Journal Article
Lenguaje:Inglés
Publicado: Informa UK Limited 2022
Materias:
Acceso en línea:https://hdl.handle.net/10568/126427
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author Awata, Luka A. O.
Ifie, Beatrice E.
Danquah, Eric
Tongoona, Pangirayi
Mahabaleswara, Suresh L.
Gowda, Manje
Marchelo-Dragga, Philip W.
Sitonik, Chelang’At
Olsen, Michael
Boddupalli, P.M.
Jumbo, McDonald Bright
author_browse Awata, Luka A. O.
Boddupalli, P.M.
Danquah, Eric
Gowda, Manje
Ifie, Beatrice E.
Jumbo, McDonald Bright
Mahabaleswara, Suresh L.
Marchelo-Dragga, Philip W.
Olsen, Michael
Sitonik, Chelang’At
Tongoona, Pangirayi
author_facet Awata, Luka A. O.
Ifie, Beatrice E.
Danquah, Eric
Tongoona, Pangirayi
Mahabaleswara, Suresh L.
Gowda, Manje
Marchelo-Dragga, Philip W.
Sitonik, Chelang’At
Olsen, Michael
Boddupalli, P.M.
Jumbo, McDonald Bright
author_sort Awata, Luka A. O.
collection Repository of Agricultural Research Outputs (CGSpace)
description Contrast between marker-assisted backcross (MABC) and doubled haploid (DH) methods in transferring genes for resistance to maize lethal necrosis (MLN) in maize (Zea mays L.) is not well understood. The MLN is caused by co-infection of maize plant by maize chlorotic mottle virus and sugarcane mosaic virus. Two maize panels consisting of four BC3F2 and six DH populations, separately developed through marker-assisted selection from crosses between susceptible CIMMYT lines and MLN-resistant donor parent (KS23-6), were used in the current study. The two populations were of different population structures with unequal sizes. Experiments were conducted under artificial MLN inoculations for two seasons in 2018. Analyses of variance revealed significant variations among genotypes in both panels (p ≤ 0.001). Levene’s and Welch’s tests found that variances and means of the BC3F2 and DH populations were highly unequal (p ≤ 0.001). The study identified genotypes with reduced MLN infections in both populations; however, lower means for MLN severity and area under disease progress curve (AUDPC) values, and higher heritability estimates were obtained in the DH populations than in the BC3F2 populations. Additionally, the DH populations showed higher relative genetic gains for resistance to MLN compared with the BC3F2 populations. The current study detected superiority of DH over MABC populations for breeding for resistance to MLN. Nevertheless, the results observed in the present study warrant further investigations using the same genetic materials with identical population sizes.
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spelling CGSpace1264272025-11-12T05:35:06Z Comparison of non-overlapping maize populations of unequal sizes for resistance to maize lethal necrosis Awata, Luka A. O. Ifie, Beatrice E. Danquah, Eric Tongoona, Pangirayi Mahabaleswara, Suresh L. Gowda, Manje Marchelo-Dragga, Philip W. Sitonik, Chelang’At Olsen, Michael Boddupalli, P.M. Jumbo, McDonald Bright backcrossing breeding lines necrosis marker-assisted selection zea mays maize Contrast between marker-assisted backcross (MABC) and doubled haploid (DH) methods in transferring genes for resistance to maize lethal necrosis (MLN) in maize (Zea mays L.) is not well understood. The MLN is caused by co-infection of maize plant by maize chlorotic mottle virus and sugarcane mosaic virus. Two maize panels consisting of four BC3F2 and six DH populations, separately developed through marker-assisted selection from crosses between susceptible CIMMYT lines and MLN-resistant donor parent (KS23-6), were used in the current study. The two populations were of different population structures with unequal sizes. Experiments were conducted under artificial MLN inoculations for two seasons in 2018. Analyses of variance revealed significant variations among genotypes in both panels (p ≤ 0.001). Levene’s and Welch’s tests found that variances and means of the BC3F2 and DH populations were highly unequal (p ≤ 0.001). The study identified genotypes with reduced MLN infections in both populations; however, lower means for MLN severity and area under disease progress curve (AUDPC) values, and higher heritability estimates were obtained in the DH populations than in the BC3F2 populations. Additionally, the DH populations showed higher relative genetic gains for resistance to MLN compared with the BC3F2 populations. The current study detected superiority of DH over MABC populations for breeding for resistance to MLN. Nevertheless, the results observed in the present study warrant further investigations using the same genetic materials with identical population sizes. 2022-07-04 2023-01-01T16:03:40Z 2023-01-01T16:03:40Z Journal Article https://hdl.handle.net/10568/126427 en Open Access application/pdf Informa UK Limited Awata, L. A. O., Ifie, B. E., Danquah, E., Tongoona, P., Suresh, L. M., Gowda, M., Marchelo-Dragga, P. W., Sitonik, C., Olsen, M., Prasanna, B. M., & Jumbo, M. B. (2021). Comparison of non-overlapping maize populations of unequal sizes for resistance to maize lethal necrosis. Journal of Crop Improvement, 36(4), 494–513. https://doi.org/10.1080/15427528.2021.1979157
spellingShingle backcrossing
breeding lines
necrosis
marker-assisted selection
zea mays
maize
Awata, Luka A. O.
Ifie, Beatrice E.
Danquah, Eric
Tongoona, Pangirayi
Mahabaleswara, Suresh L.
Gowda, Manje
Marchelo-Dragga, Philip W.
Sitonik, Chelang’At
Olsen, Michael
Boddupalli, P.M.
Jumbo, McDonald Bright
Comparison of non-overlapping maize populations of unequal sizes for resistance to maize lethal necrosis
title Comparison of non-overlapping maize populations of unequal sizes for resistance to maize lethal necrosis
title_full Comparison of non-overlapping maize populations of unequal sizes for resistance to maize lethal necrosis
title_fullStr Comparison of non-overlapping maize populations of unequal sizes for resistance to maize lethal necrosis
title_full_unstemmed Comparison of non-overlapping maize populations of unequal sizes for resistance to maize lethal necrosis
title_short Comparison of non-overlapping maize populations of unequal sizes for resistance to maize lethal necrosis
title_sort comparison of non overlapping maize populations of unequal sizes for resistance to maize lethal necrosis
topic backcrossing
breeding lines
necrosis
marker-assisted selection
zea mays
maize
url https://hdl.handle.net/10568/126427
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