Development and validation of a novel core set of KASP markers for the traits improving grain yield and adaptability of rice under direct-seeded cultivation conditions
The development and utilization of molecular-markers play an important role in genomics-assisted breeding during pyramiding of valuable genes. The aim of present study was to develop and validate a novel core-set of KASP (Kompetitive Allele-Specific PCR) markers associated with traits improving rice...
| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Elsevier
2022
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/126152 |
| _version_ | 1855536139489771520 |
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| author | Sandhu, Nitika Singh, Jasneet Singh, Gursewak Sethi, Mehak Singh, Mohini Prabha Pruthi, Gomsie Raigar, Om Prakash Kaur, Rupinder Sarao, Preetinder Singh Lore, Jagjeet Singh Singh, Uma Maheshwar Dixit, Shilpi Sagare, Deepti B. Singh, S.P. Satturu, Vanisri Singh, Vikas Kumar Kumar, Arvind |
| author_browse | Dixit, Shilpi Kaur, Rupinder Kumar, Arvind Lore, Jagjeet Singh Pruthi, Gomsie Raigar, Om Prakash Sagare, Deepti B. Sandhu, Nitika Sarao, Preetinder Singh Satturu, Vanisri Sethi, Mehak Singh, Gursewak Singh, Jasneet Singh, Mohini Prabha Singh, S.P. Singh, Uma Maheshwar Singh, Vikas Kumar |
| author_facet | Sandhu, Nitika Singh, Jasneet Singh, Gursewak Sethi, Mehak Singh, Mohini Prabha Pruthi, Gomsie Raigar, Om Prakash Kaur, Rupinder Sarao, Preetinder Singh Lore, Jagjeet Singh Singh, Uma Maheshwar Dixit, Shilpi Sagare, Deepti B. Singh, S.P. Satturu, Vanisri Singh, Vikas Kumar Kumar, Arvind |
| author_sort | Sandhu, Nitika |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The development and utilization of molecular-markers play an important role in genomics-assisted breeding during pyramiding of valuable genes. The aim of present study was to develop and validate a novel core-set of KASP (Kompetitive Allele-Specific PCR) markers associated with traits improving rice grain yield and adaptability under direct-seeded cultivation conditions. The 110 phenotypically validated KASP assays out of 171 designed KASP, include assays for biotic-resistance genes, anaerobic germination, root-traits, grain yield, lodging resistance and early-uniform emergence. The KASP assays were validated for their robustness and reliability at five different levels using diverse germplasm, segregating and advanced population, comparison with SSR markers and on F1s. The present research work will provide (i) breeding material in form of anticipated pre-direct-seeded adapted rice varieties (ii) single improved breeding line with many useful genes and (iii) KASP assay information for the useful QTL/genes providing grain yield and adaptability to rice under direct-seeded cultivation conditions. |
| format | Journal Article |
| id | CGSpace126152 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | CGSpace1261522025-11-12T05:34:46Z Development and validation of a novel core set of KASP markers for the traits improving grain yield and adaptability of rice under direct-seeded cultivation conditions Sandhu, Nitika Singh, Jasneet Singh, Gursewak Sethi, Mehak Singh, Mohini Prabha Pruthi, Gomsie Raigar, Om Prakash Kaur, Rupinder Sarao, Preetinder Singh Lore, Jagjeet Singh Singh, Uma Maheshwar Dixit, Shilpi Sagare, Deepti B. Singh, S.P. Satturu, Vanisri Singh, Vikas Kumar Kumar, Arvind abiotic stress biotic stress rice breeding methods grain yields The development and utilization of molecular-markers play an important role in genomics-assisted breeding during pyramiding of valuable genes. The aim of present study was to develop and validate a novel core-set of KASP (Kompetitive Allele-Specific PCR) markers associated with traits improving rice grain yield and adaptability under direct-seeded cultivation conditions. The 110 phenotypically validated KASP assays out of 171 designed KASP, include assays for biotic-resistance genes, anaerobic germination, root-traits, grain yield, lodging resistance and early-uniform emergence. The KASP assays were validated for their robustness and reliability at five different levels using diverse germplasm, segregating and advanced population, comparison with SSR markers and on F1s. The present research work will provide (i) breeding material in form of anticipated pre-direct-seeded adapted rice varieties (ii) single improved breeding line with many useful genes and (iii) KASP assay information for the useful QTL/genes providing grain yield and adaptability to rice under direct-seeded cultivation conditions. 2022-03 2022-12-21T08:40:34Z 2022-12-21T08:40:34Z Journal Article https://hdl.handle.net/10568/126152 en Open Access application/pdf Elsevier Sandhu, Nitika, Singh, Jasneet, Singh, Gursewak, Sethi, Mehak, Singh, Mohini Prabha, Pruthi, Gomsie, Raigar, Om Prakash, Kaur, Rupinder, Kaur, Rupinder, Sarao, Preetinder Singh, Lore, Jagjeet Singh, Singh, Uma Maheshwar, Dixit, Shilpi, Sagare, Deepti B., Singh, S.P., Satturu, Vanisri, Singh, Vikas Kumar and Kumar, Arvind. 2022. Development and validation of a novel core set of KASP markers for the traits improving grain yield and adaptability of rice under direct-seeded cultivation conditions. Genomics 114(2):110269. |
| spellingShingle | abiotic stress biotic stress rice breeding methods grain yields Sandhu, Nitika Singh, Jasneet Singh, Gursewak Sethi, Mehak Singh, Mohini Prabha Pruthi, Gomsie Raigar, Om Prakash Kaur, Rupinder Sarao, Preetinder Singh Lore, Jagjeet Singh Singh, Uma Maheshwar Dixit, Shilpi Sagare, Deepti B. Singh, S.P. Satturu, Vanisri Singh, Vikas Kumar Kumar, Arvind Development and validation of a novel core set of KASP markers for the traits improving grain yield and adaptability of rice under direct-seeded cultivation conditions |
| title | Development and validation of a novel core set of KASP markers for the traits improving grain yield and adaptability of rice under direct-seeded cultivation conditions |
| title_full | Development and validation of a novel core set of KASP markers for the traits improving grain yield and adaptability of rice under direct-seeded cultivation conditions |
| title_fullStr | Development and validation of a novel core set of KASP markers for the traits improving grain yield and adaptability of rice under direct-seeded cultivation conditions |
| title_full_unstemmed | Development and validation of a novel core set of KASP markers for the traits improving grain yield and adaptability of rice under direct-seeded cultivation conditions |
| title_short | Development and validation of a novel core set of KASP markers for the traits improving grain yield and adaptability of rice under direct-seeded cultivation conditions |
| title_sort | development and validation of a novel core set of kasp markers for the traits improving grain yield and adaptability of rice under direct seeded cultivation conditions |
| topic | abiotic stress biotic stress rice breeding methods grain yields |
| url | https://hdl.handle.net/10568/126152 |
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