Characteristics of Staphylococcus aureus isolated from patients in Busia County Referral Hospital, Kenya
Staphylococcus aureus is an important pathogen associated with hospital, community, and livestock-acquired infections, with the ability to develop resistance to antibiotics. Nasal carriage by hospital inpatients is a risk for opportunistic infections. Antibiotic susceptibility patterns, virulence ge...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
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MDPI
2022
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/125963 |
| _version_ | 1855540402438799360 |
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| author | Obanda, B.A. Cook, Elizabeth A.J. Fèvre, Eric M. Bebora, L. Ogara, W. Wang, S.-H. Gebreyes, W. Ngetich, R. Wandede, D. Muyodi, J. Blane, B. Coll, F. Harrison, E.M. Peacock, S.J. Gitao, G.C. |
| author_browse | Bebora, L. Blane, B. Coll, F. Cook, Elizabeth A.J. Fèvre, Eric M. Gebreyes, W. Gitao, G.C. Harrison, E.M. Muyodi, J. Ngetich, R. Obanda, B.A. Ogara, W. Peacock, S.J. Wandede, D. Wang, S.-H. |
| author_facet | Obanda, B.A. Cook, Elizabeth A.J. Fèvre, Eric M. Bebora, L. Ogara, W. Wang, S.-H. Gebreyes, W. Ngetich, R. Wandede, D. Muyodi, J. Blane, B. Coll, F. Harrison, E.M. Peacock, S.J. Gitao, G.C. |
| author_sort | Obanda, B.A. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Staphylococcus aureus is an important pathogen associated with hospital, community, and livestock-acquired infections, with the ability to develop resistance to antibiotics. Nasal carriage by hospital inpatients is a risk for opportunistic infections. Antibiotic susceptibility patterns, virulence genes and genetic population structure of S. aureus nasal isolates, from inpatients at Busia County Referral Hospital (BCRH) were analyzed. A total of 263 inpatients were randomly sampled, from May to July 2015. The majority of inpatients (85.9%) were treated empirically with antimicrobials, including ceftriaxone (65.8%) and metronidazole (49.8%). Thirty S. aureus isolates were cultured from 29 inpatients with a prevalence of 11% (10.3% methicillin-susceptible S. aureus (MSSA), 0.8% methicillin resistant S. aureus (MRSA)). Phenotypic and genotypic resistance was highest to penicillin-G (96.8%), trimethoprim (73.3%), and tetracycline (13.3%) with 20% of isolates classified as multidrug resistant. Virulence genes, Panton-Valentine leukocidin (pvl), toxic shock syndrome toxin-1 (tsst-1), and sasX gene were detected in 16.7%, 23.3% and 3.3% of isolates. Phylogenetic analysis showed 4 predominant clonal complexes CC152, CC8, CC80, and CC508. This study has identified that inpatients of BCRH were carriers of S. aureus harbouring virulence genes and resistance to a range of antibiotics. This may indicate a public health risk to other patients and the community. |
| format | Journal Article |
| id | CGSpace125963 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | CGSpace1259632025-12-08T10:29:22Z Characteristics of Staphylococcus aureus isolated from patients in Busia County Referral Hospital, Kenya Obanda, B.A. Cook, Elizabeth A.J. Fèvre, Eric M. Bebora, L. Ogara, W. Wang, S.-H. Gebreyes, W. Ngetich, R. Wandede, D. Muyodi, J. Blane, B. Coll, F. Harrison, E.M. Peacock, S.J. Gitao, G.C. health antimicrobial resistance pathogens hospitals antibiotics infection drug resistance public health staphylococcus aureus Staphylococcus aureus is an important pathogen associated with hospital, community, and livestock-acquired infections, with the ability to develop resistance to antibiotics. Nasal carriage by hospital inpatients is a risk for opportunistic infections. Antibiotic susceptibility patterns, virulence genes and genetic population structure of S. aureus nasal isolates, from inpatients at Busia County Referral Hospital (BCRH) were analyzed. A total of 263 inpatients were randomly sampled, from May to July 2015. The majority of inpatients (85.9%) were treated empirically with antimicrobials, including ceftriaxone (65.8%) and metronidazole (49.8%). Thirty S. aureus isolates were cultured from 29 inpatients with a prevalence of 11% (10.3% methicillin-susceptible S. aureus (MSSA), 0.8% methicillin resistant S. aureus (MRSA)). Phenotypic and genotypic resistance was highest to penicillin-G (96.8%), trimethoprim (73.3%), and tetracycline (13.3%) with 20% of isolates classified as multidrug resistant. Virulence genes, Panton-Valentine leukocidin (pvl), toxic shock syndrome toxin-1 (tsst-1), and sasX gene were detected in 16.7%, 23.3% and 3.3% of isolates. Phylogenetic analysis showed 4 predominant clonal complexes CC152, CC8, CC80, and CC508. This study has identified that inpatients of BCRH were carriers of S. aureus harbouring virulence genes and resistance to a range of antibiotics. This may indicate a public health risk to other patients and the community. 2022-12-09 2022-12-14T10:16:15Z 2022-12-14T10:16:15Z Journal Article https://hdl.handle.net/10568/125963 en Open Access MDPI Obanda, B.A., Cook, E.A.J., Fèvre, E.M., Bebora, L., Ogara, W., Wang, S.-H., Gebreyes, W., Ngetich, R., Wandede, D., Muyodi, J., Blane, B., Coll, F., Harrison, E.M., Peacock, S.J. and Gitao, G.C. 2022. Characteristics of Staphylococcus aureus isolated from patients in Busia County Referral Hospital, Kenya. Pathogens 11(12): 1504. |
| spellingShingle | health antimicrobial resistance pathogens hospitals antibiotics infection drug resistance public health staphylococcus aureus Obanda, B.A. Cook, Elizabeth A.J. Fèvre, Eric M. Bebora, L. Ogara, W. Wang, S.-H. Gebreyes, W. Ngetich, R. Wandede, D. Muyodi, J. Blane, B. Coll, F. Harrison, E.M. Peacock, S.J. Gitao, G.C. Characteristics of Staphylococcus aureus isolated from patients in Busia County Referral Hospital, Kenya |
| title | Characteristics of Staphylococcus aureus isolated from patients in Busia County Referral Hospital, Kenya |
| title_full | Characteristics of Staphylococcus aureus isolated from patients in Busia County Referral Hospital, Kenya |
| title_fullStr | Characteristics of Staphylococcus aureus isolated from patients in Busia County Referral Hospital, Kenya |
| title_full_unstemmed | Characteristics of Staphylococcus aureus isolated from patients in Busia County Referral Hospital, Kenya |
| title_short | Characteristics of Staphylococcus aureus isolated from patients in Busia County Referral Hospital, Kenya |
| title_sort | characteristics of staphylococcus aureus isolated from patients in busia county referral hospital kenya |
| topic | health antimicrobial resistance pathogens hospitals antibiotics infection drug resistance public health staphylococcus aureus |
| url | https://hdl.handle.net/10568/125963 |
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