Genomic regions associated with herbicide tolerance in a worldwide faba bean (Vicia faba L.) collection
Weeds represent one of the major constraints for faba bean crop. The identification of molecular markers associated with key genes imparting tolerance to herbicides can facilitate and fasten the efficient and effective development of herbicide tolerant cultivars. We phenotyped 140 faba bean genotype...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
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NATURE RESEARCH
2022
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/125863 |
| _version_ | 1855530134723887104 |
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| author | Abou-Khater, Lynn Maalouf, Fouad Jighly, Abdulqader Alsamman, Alsamman M. Rubiales, Diego Rispail, Nicolas Hu, Jinguo Ma, Yu Balech, Rind Hamwieh, Aladdin Baum, Michael Agrawal, Shiv Kumar |
| author_browse | Abou-Khater, Lynn Agrawal, Shiv Kumar Alsamman, Alsamman M. Balech, Rind Baum, Michael Hamwieh, Aladdin Hu, Jinguo Jighly, Abdulqader Ma, Yu Maalouf, Fouad Rispail, Nicolas Rubiales, Diego |
| author_facet | Abou-Khater, Lynn Maalouf, Fouad Jighly, Abdulqader Alsamman, Alsamman M. Rubiales, Diego Rispail, Nicolas Hu, Jinguo Ma, Yu Balech, Rind Hamwieh, Aladdin Baum, Michael Agrawal, Shiv Kumar |
| author_sort | Abou-Khater, Lynn |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Weeds represent one of the major constraints for faba bean crop. The identification of molecular markers associated with key genes imparting tolerance to herbicides can facilitate and fasten the efficient and effective development of herbicide tolerant cultivars. We phenotyped 140 faba bean genotypes in three open field experiments at two locations in Lebanon and Morocco against three herbicide treatments (T1 metribuzin 250 g ai/ha; T2 imazethapyr 75 g ai/ha; T3 untreated) and one in greenhouse where T1 and T3 were applied. The same set was genotyped using genotyping by sequencing (GBS) which yield 10,794 high quality single nucleotide polymorphisms (SNPs). ADMIXTURE software was used to infer the population structure which revealed two ancestral subpopulations. To identify SNPs associated with phenological and yield related traits under herbicide treatments, Single-trait (ST) and Multi-trait (MT) Genome Wide Association Studies (GWAS) were fitted using GEMMA software, showing 10 and 14 highly significant associations, respectively. Genomic sequences containing herbicide tolerance associated SNPs were aligned against the NCBI database using BLASTX tool using default parameters to annotate candidate genes underlying the causal variants. SNPs from acidic endochitinase, LRR receptor-like serine/threonine-protein kinase RCH1, probable serine/threonine-protein kinase NAK, malate dehydrogenase, photosystem I core protein PsaA and MYB-related protein P-like were significantly associated with herbicide tolerance traits. |
| format | Journal Article |
| id | CGSpace125863 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | NATURE RESEARCH |
| publisherStr | NATURE RESEARCH |
| record_format | dspace |
| spelling | CGSpace1258632026-01-15T02:11:45Z Genomic regions associated with herbicide tolerance in a worldwide faba bean (Vicia faba L.) collection Abou-Khater, Lynn Maalouf, Fouad Jighly, Abdulqader Alsamman, Alsamman M. Rubiales, Diego Rispail, Nicolas Hu, Jinguo Ma, Yu Balech, Rind Hamwieh, Aladdin Baum, Michael Agrawal, Shiv Kumar biotechnology food security health nutrition genetics goal 2 zero hunger vicia faba l. Weeds represent one of the major constraints for faba bean crop. The identification of molecular markers associated with key genes imparting tolerance to herbicides can facilitate and fasten the efficient and effective development of herbicide tolerant cultivars. We phenotyped 140 faba bean genotypes in three open field experiments at two locations in Lebanon and Morocco against three herbicide treatments (T1 metribuzin 250 g ai/ha; T2 imazethapyr 75 g ai/ha; T3 untreated) and one in greenhouse where T1 and T3 were applied. The same set was genotyped using genotyping by sequencing (GBS) which yield 10,794 high quality single nucleotide polymorphisms (SNPs). ADMIXTURE software was used to infer the population structure which revealed two ancestral subpopulations. To identify SNPs associated with phenological and yield related traits under herbicide treatments, Single-trait (ST) and Multi-trait (MT) Genome Wide Association Studies (GWAS) were fitted using GEMMA software, showing 10 and 14 highly significant associations, respectively. Genomic sequences containing herbicide tolerance associated SNPs were aligned against the NCBI database using BLASTX tool using default parameters to annotate candidate genes underlying the causal variants. SNPs from acidic endochitinase, LRR receptor-like serine/threonine-protein kinase RCH1, probable serine/threonine-protein kinase NAK, malate dehydrogenase, photosystem I core protein PsaA and MYB-related protein P-like were significantly associated with herbicide tolerance traits. 2022-12-08T14:46:47Z 2022-12-08T14:46:47Z Journal Article https://hdl.handle.net/10568/125863 en Open Access application/pdf NATURE RESEARCH Lynn Abou-Khater, Fouad Maalouf, Abdulqader Jighly, Alsamman M. Alsamman, Diego Rubiales, Nicolas Rispail, Jinguo Hu, Yu Ma, Rind Balech, Aladdin Hamwieh, Michael Baum, Shiv Kumar Agrawal. (7/1/2022). Genomic regions associated with herbicide tolerance in a worldwide faba bean (Vicia faba L. ) collection. Scientific Reports, 158. |
| spellingShingle | biotechnology food security health nutrition genetics goal 2 zero hunger vicia faba l. Abou-Khater, Lynn Maalouf, Fouad Jighly, Abdulqader Alsamman, Alsamman M. Rubiales, Diego Rispail, Nicolas Hu, Jinguo Ma, Yu Balech, Rind Hamwieh, Aladdin Baum, Michael Agrawal, Shiv Kumar Genomic regions associated with herbicide tolerance in a worldwide faba bean (Vicia faba L.) collection |
| title | Genomic regions associated with herbicide tolerance in a worldwide faba bean (Vicia faba L.) collection |
| title_full | Genomic regions associated with herbicide tolerance in a worldwide faba bean (Vicia faba L.) collection |
| title_fullStr | Genomic regions associated with herbicide tolerance in a worldwide faba bean (Vicia faba L.) collection |
| title_full_unstemmed | Genomic regions associated with herbicide tolerance in a worldwide faba bean (Vicia faba L.) collection |
| title_short | Genomic regions associated with herbicide tolerance in a worldwide faba bean (Vicia faba L.) collection |
| title_sort | genomic regions associated with herbicide tolerance in a worldwide faba bean vicia faba l collection |
| topic | biotechnology food security health nutrition genetics goal 2 zero hunger vicia faba l. |
| url | https://hdl.handle.net/10568/125863 |
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