Six SNPs and a TTG indel in sheep desmoglein 4 gene are in complete linkage disequilibrium
Desmoglein 4 (DSG4) plays an important role in the regulation of growth and differentiation of hair follicles in mammals. In this study, a 755 bp long segment of DSG4 was screened in 544 sheep sampled from nine Chinese indigenous breeds and two Western breeds using PCR-SSCP assay with three differen...
| Main Authors: | , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
2011
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| Online Access: | https://hdl.handle.net/10568/12585 |
| _version_ | 1855519695303606272 |
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| author | Yue-Hui Ma Ge, Zhang Yuan-yue, Zhang Weijun, Guan Qian-Jun Zhao Hui-jing, Zhao Han Jianlin |
| author_browse | Ge, Zhang Han Jianlin Hui-jing, Zhao Qian-Jun Zhao Weijun, Guan Yuan-yue, Zhang Yue-Hui Ma |
| author_facet | Yue-Hui Ma Ge, Zhang Yuan-yue, Zhang Weijun, Guan Qian-Jun Zhao Hui-jing, Zhao Han Jianlin |
| author_sort | Yue-Hui Ma |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Desmoglein 4 (DSG4) plays an important role in the regulation of growth and differentiation of hair follicles in mammals. In this study, a 755 bp long segment of DSG4 was screened in 544 sheep sampled from nine Chinese indigenous breeds and two Western breeds using PCR-SSCP assay with three different pairs of primers. Two of the three fragments showed polymorphisms with genotypes defined as AA, AB, BB and BC, and DD, DE, and EE, respectively. Interestingly, polymorphisms in these two fragments were in strong linkage disequilibrium. Only three haplotypes were found, of which haplotype AD determined by alleles A and D was the major one in all breeds, while haplotype BE was only found in Chinese breeds that possess divergent frequencies ranging from 0.02 to 0.43; haplotype CD was very rare and present in only one Chinese sheep. Sequences of the three haplotypes showed seven single nucleotide polymorphisms (SNPs) and a TTG insertion/deletion (indel), leading to five amino acid substitutions and a glycine indel. Our study provides valuable genetic markers in evaluating the impact of the DSG4 gene on wool traits in sheep. |
| format | Journal Article |
| id | CGSpace12585 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2011 |
| publishDateRange | 2011 |
| publishDateSort | 2011 |
| record_format | dspace |
| spelling | CGSpace125852024-01-09T09:49:58Z Six SNPs and a TTG indel in sheep desmoglein 4 gene are in complete linkage disequilibrium Yue-Hui Ma Ge, Zhang Yuan-yue, Zhang Weijun, Guan Qian-Jun Zhao Hui-jing, Zhao Han Jianlin Desmoglein 4 (DSG4) plays an important role in the regulation of growth and differentiation of hair follicles in mammals. In this study, a 755 bp long segment of DSG4 was screened in 544 sheep sampled from nine Chinese indigenous breeds and two Western breeds using PCR-SSCP assay with three different pairs of primers. Two of the three fragments showed polymorphisms with genotypes defined as AA, AB, BB and BC, and DD, DE, and EE, respectively. Interestingly, polymorphisms in these two fragments were in strong linkage disequilibrium. Only three haplotypes were found, of which haplotype AD determined by alleles A and D was the major one in all breeds, while haplotype BE was only found in Chinese breeds that possess divergent frequencies ranging from 0.02 to 0.43; haplotype CD was very rare and present in only one Chinese sheep. Sequences of the three haplotypes showed seven single nucleotide polymorphisms (SNPs) and a TTG insertion/deletion (indel), leading to five amino acid substitutions and a glycine indel. Our study provides valuable genetic markers in evaluating the impact of the DSG4 gene on wool traits in sheep. 2011-07-18 2012-01-01T17:46:49Z 2012-01-01T17:46:49Z Journal Article https://hdl.handle.net/10568/12585 en Open Access Ge Zhang, Yue-hui Ma, Yuan-yue Zhang, Wei-jun Guan, Qian-jun Zhao, Hui-jing Zhao and Jian-lin Han. 2011. Six SNPs and a TTG indel in sheep desmoglein 4 gene are in complete linkage disequilibrium. African Journal of Biotechnology 10(36):6852-6856. |
| spellingShingle | Yue-Hui Ma Ge, Zhang Yuan-yue, Zhang Weijun, Guan Qian-Jun Zhao Hui-jing, Zhao Han Jianlin Six SNPs and a TTG indel in sheep desmoglein 4 gene are in complete linkage disequilibrium |
| title | Six SNPs and a TTG indel in sheep desmoglein 4 gene are in complete linkage disequilibrium |
| title_full | Six SNPs and a TTG indel in sheep desmoglein 4 gene are in complete linkage disequilibrium |
| title_fullStr | Six SNPs and a TTG indel in sheep desmoglein 4 gene are in complete linkage disequilibrium |
| title_full_unstemmed | Six SNPs and a TTG indel in sheep desmoglein 4 gene are in complete linkage disequilibrium |
| title_short | Six SNPs and a TTG indel in sheep desmoglein 4 gene are in complete linkage disequilibrium |
| title_sort | six snps and a ttg indel in sheep desmoglein 4 gene are in complete linkage disequilibrium |
| url | https://hdl.handle.net/10568/12585 |
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