Analysis of genetic diversity of banana weevils (Cosmopolites sordidus) (Coleoptera: Curculionidae) using tanscriptome-derived simple sequence repeat markers
The banana weevil, Cosmopolites sordidus (Germar) (Coleoptera: Curculionidae) is an economically important insect pest of bananas. It causes up to 100% yield losses and substantial lifespan reduction in bananas. Advances in genomics, proteomics, and sequencing technologies have provided powerful pat...
| Autores principales: | , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
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Oxford University Press
2022
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| Acceso en línea: | https://hdl.handle.net/10568/125793 |
| _version_ | 1855524829121216512 |
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| author | Milton, Ali Muhanguzi, Dennis Male, Allan Kajubi, Ali Buah, Stephen Kubiriba, Jerome Tumuhimbise, Robooni |
| author_browse | Buah, Stephen Kajubi, Ali Kubiriba, Jerome Male, Allan Milton, Ali Muhanguzi, Dennis Tumuhimbise, Robooni |
| author_facet | Milton, Ali Muhanguzi, Dennis Male, Allan Kajubi, Ali Buah, Stephen Kubiriba, Jerome Tumuhimbise, Robooni |
| author_sort | Milton, Ali |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The banana weevil, Cosmopolites sordidus (Germar) (Coleoptera: Curculionidae) is an economically important insect pest of bananas. It causes up to 100% yield losses and substantial lifespan reduction in bananas. Advances in genomics, proteomics, and sequencing technologies have provided powerful pathways to genotyping disastrous pests such as C. sordidus. However, such technologies are often not available to the majority of rural subtropical African banana growers and pest control managers. This study was therefore motivated by the need to create cheap and easily accessible C. sordidus genotyping methods that could be deployed by banana pest control managers to the benefit of C. sordidus control programs in the tropics where such advanced technologies are not readily accessible. We used an in-house C. sordidus transcriptome from the an-ongoing study from which we mined an array of simple sequence repeat (SSR) markers. Of these, six highly polymorphic transcriptome-derived SSR markers were used to successfully genotype within and among banana weevil population genetic diversity of 12 C. sordidus populations collected from four banana-growing agro-ecological zones (AEZs) in Uganda. The developed transcriptome-derived SSR markers can be used by researchers in population genetics for characterization of the C. sordidus and identification of new genes that are linked to traits of particular interest. The significant genetic diversity revealed in C. sordidus provides pertinent information for integrated pest management strategies. |
| format | Journal Article |
| id | CGSpace125793 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Oxford University Press |
| publisherStr | Oxford University Press |
| record_format | dspace |
| spelling | CGSpace1257932025-11-11T18:48:39Z Analysis of genetic diversity of banana weevils (Cosmopolites sordidus) (Coleoptera: Curculionidae) using tanscriptome-derived simple sequence repeat markers Milton, Ali Muhanguzi, Dennis Male, Allan Kajubi, Ali Buah, Stephen Kubiriba, Jerome Tumuhimbise, Robooni curculionidae cosmopolites sordidus microsatellites genotyping methods musa genetic variation plant pests microsatélites genotipado plagas de plantas The banana weevil, Cosmopolites sordidus (Germar) (Coleoptera: Curculionidae) is an economically important insect pest of bananas. It causes up to 100% yield losses and substantial lifespan reduction in bananas. Advances in genomics, proteomics, and sequencing technologies have provided powerful pathways to genotyping disastrous pests such as C. sordidus. However, such technologies are often not available to the majority of rural subtropical African banana growers and pest control managers. This study was therefore motivated by the need to create cheap and easily accessible C. sordidus genotyping methods that could be deployed by banana pest control managers to the benefit of C. sordidus control programs in the tropics where such advanced technologies are not readily accessible. We used an in-house C. sordidus transcriptome from the an-ongoing study from which we mined an array of simple sequence repeat (SSR) markers. Of these, six highly polymorphic transcriptome-derived SSR markers were used to successfully genotype within and among banana weevil population genetic diversity of 12 C. sordidus populations collected from four banana-growing agro-ecological zones (AEZs) in Uganda. The developed transcriptome-derived SSR markers can be used by researchers in population genetics for characterization of the C. sordidus and identification of new genes that are linked to traits of particular interest. The significant genetic diversity revealed in C. sordidus provides pertinent information for integrated pest management strategies. 2022-04-13 2022-12-06T09:57:22Z 2022-12-06T09:57:22Z Journal Article https://hdl.handle.net/10568/125793 en Open Access application/pdf Oxford University Press Milton, A.; Muhanguzi, D.; Male, A.; Kajubi, A.; Buah, S.; Kubiriba, J.; Tumuhimbise, R. (2022) Analysis of genedic diversity of banana weevils (Cosmopolites sordidus) (Coleoptera: Curculionidae) using tanscriptome-derived simple sequence repeat markers. Journal of Economic Entomology 115(2) p. 637-646. ISSN: 1938-291X |
| spellingShingle | curculionidae cosmopolites sordidus microsatellites genotyping methods musa genetic variation plant pests microsatélites genotipado plagas de plantas Milton, Ali Muhanguzi, Dennis Male, Allan Kajubi, Ali Buah, Stephen Kubiriba, Jerome Tumuhimbise, Robooni Analysis of genetic diversity of banana weevils (Cosmopolites sordidus) (Coleoptera: Curculionidae) using tanscriptome-derived simple sequence repeat markers |
| title | Analysis of genetic diversity of banana weevils (Cosmopolites sordidus) (Coleoptera: Curculionidae) using tanscriptome-derived simple sequence repeat markers |
| title_full | Analysis of genetic diversity of banana weevils (Cosmopolites sordidus) (Coleoptera: Curculionidae) using tanscriptome-derived simple sequence repeat markers |
| title_fullStr | Analysis of genetic diversity of banana weevils (Cosmopolites sordidus) (Coleoptera: Curculionidae) using tanscriptome-derived simple sequence repeat markers |
| title_full_unstemmed | Analysis of genetic diversity of banana weevils (Cosmopolites sordidus) (Coleoptera: Curculionidae) using tanscriptome-derived simple sequence repeat markers |
| title_short | Analysis of genetic diversity of banana weevils (Cosmopolites sordidus) (Coleoptera: Curculionidae) using tanscriptome-derived simple sequence repeat markers |
| title_sort | analysis of genetic diversity of banana weevils cosmopolites sordidus coleoptera curculionidae using tanscriptome derived simple sequence repeat markers |
| topic | curculionidae cosmopolites sordidus microsatellites genotyping methods musa genetic variation plant pests microsatélites genotipado plagas de plantas |
| url | https://hdl.handle.net/10568/125793 |
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