Novel Genomic Regions Linked to Ascochyta Blight Resistance in Two Differentially Resistant Cultivars of Chickpea
Ascochyta blight (AB), caused by the fungal pathogen Ascochyta rabiei, is a devastating foliar disease of chickpea (Cicer arietinum L.). The genotyping-by-sequencing (GBS)-based approach was deployed for mapping QTLs associated with AB resistance in chickpea in two recombinant inbred line population...
| Autores principales: | , , , , , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Frontiers Media
2022
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/125765 |
| _version_ | 1855520537073156096 |
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| author | Alo, Fida Ran, Anupalli Baum, Michael Singh, Sarvjeet Kehel, Zakaria Rani, Upasana Udupa, Sripada M. Al-Shamaa, Khaled Alsamman, Alsamman M. Istanbuli, Tawffiq Attar, Basem Hamwieh, Aladdin Amri, Ahmed |
| author_browse | Al-Shamaa, Khaled Alo, Fida Alsamman, Alsamman M. Amri, Ahmed Attar, Basem Baum, Michael Hamwieh, Aladdin Istanbuli, Tawffiq Kehel, Zakaria Ran, Anupalli Rani, Upasana Singh, Sarvjeet Udupa, Sripada M. |
| author_facet | Alo, Fida Ran, Anupalli Baum, Michael Singh, Sarvjeet Kehel, Zakaria Rani, Upasana Udupa, Sripada M. Al-Shamaa, Khaled Alsamman, Alsamman M. Istanbuli, Tawffiq Attar, Basem Hamwieh, Aladdin Amri, Ahmed |
| author_sort | Alo, Fida |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Ascochyta blight (AB), caused by the fungal pathogen Ascochyta rabiei, is a devastating foliar disease of chickpea (Cicer arietinum L.). The genotyping-by-sequencing (GBS)-based approach was deployed for mapping QTLs associated with AB resistance in chickpea in two recombinant inbred line populations derived from two crosses (AB3279 derived from ILC 1929 × ILC 3279 and AB482 derived from ILC 1929 × ILC 482) and tested in six different environments. Twenty-one different genomic regions linked to AB resistance were identified in regions CalG02 and CalG04 in both populations AB3279 and AB482. These regions contain 1,118 SNPs significantly associated with AB resistance (p ≤ 0.001), which explained 11.2–39.3% of the phenotypic variation (PVE). Nine of the AB resistance-associated genomic regions were newly detected in this study, while twelve regions were known from previous AB studies. The proposed physical map narrows down AB resistance to consistent genomic regions identified across different environments. Gene ontology (GO) assigned these QTLs to 319 genes, many of which were associated with stress and disease resistance, and with most important genes belonging to resistance gene families such as leucine-rich repeat (LRR) and transcription factor families. Our results indicate that the flowering-associated gene GIGANTEA is a possible key factor in AB resistance in chickpea. The results have identified AB resistance-associated regions on the physical genetic map of chickpea and allowed for the identification of associated markers that will help in breeding of AB-resistant varieties. |
| format | Journal Article |
| id | CGSpace125765 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Frontiers Media |
| publisherStr | Frontiers Media |
| record_format | dspace |
| spelling | CGSpace1257652026-01-21T02:04:21Z Novel Genomic Regions Linked to Ascochyta Blight Resistance in Two Differentially Resistant Cultivars of Chickpea Alo, Fida Ran, Anupalli Baum, Michael Singh, Sarvjeet Kehel, Zakaria Rani, Upasana Udupa, Sripada M. Al-Shamaa, Khaled Alsamman, Alsamman M. Istanbuli, Tawffiq Attar, Basem Hamwieh, Aladdin Amri, Ahmed food security health nutrition chickpeas goal 2 zero hunger chickpea ascochyta blight resistance quantitative trait loci (qtls) genotyping-by-sequencing (gbs) Ascochyta blight (AB), caused by the fungal pathogen Ascochyta rabiei, is a devastating foliar disease of chickpea (Cicer arietinum L.). The genotyping-by-sequencing (GBS)-based approach was deployed for mapping QTLs associated with AB resistance in chickpea in two recombinant inbred line populations derived from two crosses (AB3279 derived from ILC 1929 × ILC 3279 and AB482 derived from ILC 1929 × ILC 482) and tested in six different environments. Twenty-one different genomic regions linked to AB resistance were identified in regions CalG02 and CalG04 in both populations AB3279 and AB482. These regions contain 1,118 SNPs significantly associated with AB resistance (p ≤ 0.001), which explained 11.2–39.3% of the phenotypic variation (PVE). Nine of the AB resistance-associated genomic regions were newly detected in this study, while twelve regions were known from previous AB studies. The proposed physical map narrows down AB resistance to consistent genomic regions identified across different environments. Gene ontology (GO) assigned these QTLs to 319 genes, many of which were associated with stress and disease resistance, and with most important genes belonging to resistance gene families such as leucine-rich repeat (LRR) and transcription factor families. Our results indicate that the flowering-associated gene GIGANTEA is a possible key factor in AB resistance in chickpea. The results have identified AB resistance-associated regions on the physical genetic map of chickpea and allowed for the identification of associated markers that will help in breeding of AB-resistant varieties. 2022-12-02T16:59:18Z 2022-12-02T16:59:18Z Journal Article https://hdl.handle.net/10568/125765 en Open Access application/pdf Frontiers Media Fida Alo, Anupalli Ran, Michael Baum, Sarvjeet Singh, Zakaria Kehel, Upasana Rani, Sripada M. Udupa, Khaled Al-Shamaa, Alsamman M. Alsamman, Tawffiq Istanbuli, Basem Attar, Aladdin Hamwieh, Ahmed Amri. (25/4/2022). Novel Genomic Regions Linked to Ascochyta Blight Resistance in Two Differentially Resistant Cultivars of Chickpea. Frontiers in Plant Science, 13. |
| spellingShingle | food security health nutrition chickpeas goal 2 zero hunger chickpea ascochyta blight resistance quantitative trait loci (qtls) genotyping-by-sequencing (gbs) Alo, Fida Ran, Anupalli Baum, Michael Singh, Sarvjeet Kehel, Zakaria Rani, Upasana Udupa, Sripada M. Al-Shamaa, Khaled Alsamman, Alsamman M. Istanbuli, Tawffiq Attar, Basem Hamwieh, Aladdin Amri, Ahmed Novel Genomic Regions Linked to Ascochyta Blight Resistance in Two Differentially Resistant Cultivars of Chickpea |
| title | Novel Genomic Regions Linked to Ascochyta Blight Resistance in Two Differentially Resistant Cultivars of Chickpea |
| title_full | Novel Genomic Regions Linked to Ascochyta Blight Resistance in Two Differentially Resistant Cultivars of Chickpea |
| title_fullStr | Novel Genomic Regions Linked to Ascochyta Blight Resistance in Two Differentially Resistant Cultivars of Chickpea |
| title_full_unstemmed | Novel Genomic Regions Linked to Ascochyta Blight Resistance in Two Differentially Resistant Cultivars of Chickpea |
| title_short | Novel Genomic Regions Linked to Ascochyta Blight Resistance in Two Differentially Resistant Cultivars of Chickpea |
| title_sort | novel genomic regions linked to ascochyta blight resistance in two differentially resistant cultivars of chickpea |
| topic | food security health nutrition chickpeas goal 2 zero hunger chickpea ascochyta blight resistance quantitative trait loci (qtls) genotyping-by-sequencing (gbs) |
| url | https://hdl.handle.net/10568/125765 |
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