Diverged subpopulations in tropical Urochloa (Brachiaria) forage species indicate a role for facultative apomixis and varying ploidy in their population structure and evolution
Background Urochloa (syn. Brachiaria) is a genus of tropical grasses sown as forage feedstock, particularly in marginal soils. We aimed to clarify the genetic diversity and population structure in Urochloa species to understand better how population evolution relates to ploidy level and occurrence o...
| Autores principales: | , , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Oxford University Press
2022
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| Acceso en línea: | https://hdl.handle.net/10568/125352 |
| _version_ | 1855541180350070784 |
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| author | Higgins, Janet Tomaszewska, Paulina Pellny, Till K. Castiblanco, Valheria Arango, Jacobo Tohme, Joseph M. Schwarzacher, Trude Mitchell, Rowan A. Heslop-Harrison, J.S. Vega, Jose J. de |
| author_browse | Arango, Jacobo Castiblanco, Valheria Heslop-Harrison, J.S. Higgins, Janet Mitchell, Rowan A. Pellny, Till K. Schwarzacher, Trude Tohme, Joseph M. Tomaszewska, Paulina Vega, Jose J. de |
| author_facet | Higgins, Janet Tomaszewska, Paulina Pellny, Till K. Castiblanco, Valheria Arango, Jacobo Tohme, Joseph M. Schwarzacher, Trude Mitchell, Rowan A. Heslop-Harrison, J.S. Vega, Jose J. de |
| author_sort | Higgins, Janet |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Background
Urochloa (syn. Brachiaria) is a genus of tropical grasses sown as forage feedstock, particularly in marginal soils. We aimed to clarify the genetic diversity and population structure in Urochloa species to understand better how population evolution relates to ploidy level and occurrence of apomictic reproduction.
Methods
We explored the genetic diversity of 111 accessions from the five Urochloa species used to develop commercial cultivars. These accessions were conserved from wild materials collected at their centre of origin in Africa, and tentatively represent the complete Urochloa gene pool used in breeding programmes. We used RNA-seq to generate 1.1 million SNP loci. We employed genetic admixture, principal component, and phylogenetic analysis for defining subpopulations.
Results
We observed three highly differentiated subpopulations in U. brizantha, which were unrelated to ploidy; one intermixed with U. decumbens, and two diverged from the former and the other species in the complex. We also observed two subpopulations in U. humidicola, unrelated to ploidy; one subpopulation had fewer accessions but included the only characterised sexual accession in the species. Our results also supported a division of U. decumbens between diploids and polyploids, and no subpopulations within U. ruziziensis and U. maxima.
Conclusions
Polyploid U. decumbens are more related to polyploid U. brizantha than to diploid U. decumbens, which supports the divergence of both polyploid groups from a common tetraploid ancestor and evidences of the hybridisation barrier of ploidy. The three differentiated subpopulations of apomictic polyploid U. brizantha accessions constitute diverged ecotypes, which can be likely utilised in hybrid breeding. Subpopulations were not observed in non-apomictic U. ruziziensis. Sexual Urochloa polyploids were not found (U. brizantha, U. decumbens) or were limited to small subpopulations (U. humidicola). The subpopulation structure observed in the Urochloa sexual–apomictic multiploidy complexes supports geographical parthenogenesis, where the polyploid genotypes exploit the evolutionary advantage of apomixis, i.e. uniparental reproduction and clonality, to occupy extensive geographical areas. |
| format | Journal Article |
| id | CGSpace125352 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Oxford University Press |
| publisherStr | Oxford University Press |
| record_format | dspace |
| spelling | CGSpace1253522025-11-11T19:07:44Z Diverged subpopulations in tropical Urochloa (Brachiaria) forage species indicate a role for facultative apomixis and varying ploidy in their population structure and evolution Higgins, Janet Tomaszewska, Paulina Pellny, Till K. Castiblanco, Valheria Arango, Jacobo Tohme, Joseph M. Schwarzacher, Trude Mitchell, Rowan A. Heslop-Harrison, J.S. Vega, Jose J. de grasslands plant breeding forage rna sequence apomixis parthenogenesis polyploidy praderas fitomejoramiento forrajes secuencia de arn Background Urochloa (syn. Brachiaria) is a genus of tropical grasses sown as forage feedstock, particularly in marginal soils. We aimed to clarify the genetic diversity and population structure in Urochloa species to understand better how population evolution relates to ploidy level and occurrence of apomictic reproduction. Methods We explored the genetic diversity of 111 accessions from the five Urochloa species used to develop commercial cultivars. These accessions were conserved from wild materials collected at their centre of origin in Africa, and tentatively represent the complete Urochloa gene pool used in breeding programmes. We used RNA-seq to generate 1.1 million SNP loci. We employed genetic admixture, principal component, and phylogenetic analysis for defining subpopulations. Results We observed three highly differentiated subpopulations in U. brizantha, which were unrelated to ploidy; one intermixed with U. decumbens, and two diverged from the former and the other species in the complex. We also observed two subpopulations in U. humidicola, unrelated to ploidy; one subpopulation had fewer accessions but included the only characterised sexual accession in the species. Our results also supported a division of U. decumbens between diploids and polyploids, and no subpopulations within U. ruziziensis and U. maxima. Conclusions Polyploid U. decumbens are more related to polyploid U. brizantha than to diploid U. decumbens, which supports the divergence of both polyploid groups from a common tetraploid ancestor and evidences of the hybridisation barrier of ploidy. The three differentiated subpopulations of apomictic polyploid U. brizantha accessions constitute diverged ecotypes, which can be likely utilised in hybrid breeding. Subpopulations were not observed in non-apomictic U. ruziziensis. Sexual Urochloa polyploids were not found (U. brizantha, U. decumbens) or were limited to small subpopulations (U. humidicola). The subpopulation structure observed in the Urochloa sexual–apomictic multiploidy complexes supports geographical parthenogenesis, where the polyploid genotypes exploit the evolutionary advantage of apomixis, i.e. uniparental reproduction and clonality, to occupy extensive geographical areas. 2022-11-17 2022-11-07T14:35:42Z 2022-11-07T14:35:42Z Journal Article https://hdl.handle.net/10568/125352 en Open Access application/pdf Oxford University Press Higgins, J.; Tomaszewska, P.; Pellny, T.K.; Castiblanco, V.; Arango, J.; Tohme, J.; Schwarzacher, T.; Mitchell, R.A.; Heslop-Harrison, J.S.; De Vega, J.J. (2022). Diverged subpopulations in tropical Urochloa (Brachiaria) forage species indicate a role for facultative apomixis and varying ploidy in their population structure and evolution. Annals of Botany, mcac115. ISSN: 1095-8290 |
| spellingShingle | grasslands plant breeding forage rna sequence apomixis parthenogenesis polyploidy praderas fitomejoramiento forrajes secuencia de arn Higgins, Janet Tomaszewska, Paulina Pellny, Till K. Castiblanco, Valheria Arango, Jacobo Tohme, Joseph M. Schwarzacher, Trude Mitchell, Rowan A. Heslop-Harrison, J.S. Vega, Jose J. de Diverged subpopulations in tropical Urochloa (Brachiaria) forage species indicate a role for facultative apomixis and varying ploidy in their population structure and evolution |
| title | Diverged subpopulations in tropical Urochloa (Brachiaria) forage species indicate a role for facultative apomixis and varying ploidy in their population structure and evolution |
| title_full | Diverged subpopulations in tropical Urochloa (Brachiaria) forage species indicate a role for facultative apomixis and varying ploidy in their population structure and evolution |
| title_fullStr | Diverged subpopulations in tropical Urochloa (Brachiaria) forage species indicate a role for facultative apomixis and varying ploidy in their population structure and evolution |
| title_full_unstemmed | Diverged subpopulations in tropical Urochloa (Brachiaria) forage species indicate a role for facultative apomixis and varying ploidy in their population structure and evolution |
| title_short | Diverged subpopulations in tropical Urochloa (Brachiaria) forage species indicate a role for facultative apomixis and varying ploidy in their population structure and evolution |
| title_sort | diverged subpopulations in tropical urochloa brachiaria forage species indicate a role for facultative apomixis and varying ploidy in their population structure and evolution |
| topic | grasslands plant breeding forage rna sequence apomixis parthenogenesis polyploidy praderas fitomejoramiento forrajes secuencia de arn |
| url | https://hdl.handle.net/10568/125352 |
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