Exogenous and endogenous plant growth regulators’ effect on regeneration of selected African sweetpotato (Ipomoea batatas L. Lam) cultivars
Optimization of plant regeneration protocol is a first critical step to facilitate techniques such as transgenesis, cisgenesis and genome editing. In this study, we assessed: i) influence of explant type on regeneration efficiency (RE); ii) RE of 33 sub-Saharan African sweetpotato cultivars; and iii...
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| Format: | Journal Article |
| Language: | Inglés |
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International Society for Horticultural Science
2022
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| Online Access: | https://hdl.handle.net/10568/125337 |
| _version_ | 1855526913262485504 |
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| author | Wamalwa, L. Tovar, J.C. Indieka, S. Torto, B. Ghislain, M. |
| author_browse | Ghislain, M. Indieka, S. Torto, B. Tovar, J.C. Wamalwa, L. |
| author_facet | Wamalwa, L. Tovar, J.C. Indieka, S. Torto, B. Ghislain, M. |
| author_sort | Wamalwa, L. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Optimization of plant regeneration protocol is a first critical step to facilitate techniques such as transgenesis, cisgenesis and genome editing. In this study, we assessed: i) influence of explant type on regeneration efficiency (RE); ii) RE of 33 sub-Saharan African sweetpotato cultivars; and iii) levels of endogenous zeatin riboside (ZR), indole-3-acetic acid (IAA) and their correlation to RE. Indirect organogenesis was conducted using a 2-step protocol: step-1, explants were cultured on MS containing 0.05 mg L-1 2,4-D for 2 days and transferred to step-2 with MS with 0.2 mg L-1 cytokinin for 60 days. Highest RE was obtained from leaf with petiole explants. High RE of 60–87% was obtained for 3 cultivars, moderate RE >40% for 7 cultivars and low RE <10% for 17 cultivars. Results indicated that exogenous plant growth regulators (PGRs) influenced RE, with kinetin and ZR inducing higher RE than thidiazuron (TDZ), though this was genotype dependent. Of the 6 cultivars screened for endogenous PGRs, the Tanzanian cultivar ‘Carrot’, which had with lowest RE, had the highest IAA:ZR ratio. Correlation between endogenous IAA and RE was strongly negative while weakly positive for ZR, which suggests that endogenous PGRs make a minor contribution to RE. In conclusion, 21% of cultivars with RE >40% were amenable to the 2-step protocol on application of exogenous PGRs; endogenous IAA and ZR levels are genotype-dependent, and higher endogenous IAA levels may contribute to inhibiting regeneration. For the remaining recalcitrant cultivars, we recommend further optimization, paying particular attention to endogenous PGRs that could have synergistic and antagonistic interactions with exogenous PGRs. |
| format | Journal Article |
| id | CGSpace125337 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | International Society for Horticultural Science |
| publisherStr | International Society for Horticultural Science |
| record_format | dspace |
| spelling | CGSpace1253372023-12-08T19:36:04Z Exogenous and endogenous plant growth regulators’ effect on regeneration of selected African sweetpotato (Ipomoea batatas L. Lam) cultivars Wamalwa, L. Tovar, J.C. Indieka, S. Torto, B. Ghislain, M. sweet potatoes organogenesis auxins cytokinins genetic improvement Optimization of plant regeneration protocol is a first critical step to facilitate techniques such as transgenesis, cisgenesis and genome editing. In this study, we assessed: i) influence of explant type on regeneration efficiency (RE); ii) RE of 33 sub-Saharan African sweetpotato cultivars; and iii) levels of endogenous zeatin riboside (ZR), indole-3-acetic acid (IAA) and their correlation to RE. Indirect organogenesis was conducted using a 2-step protocol: step-1, explants were cultured on MS containing 0.05 mg L-1 2,4-D for 2 days and transferred to step-2 with MS with 0.2 mg L-1 cytokinin for 60 days. Highest RE was obtained from leaf with petiole explants. High RE of 60–87% was obtained for 3 cultivars, moderate RE >40% for 7 cultivars and low RE <10% for 17 cultivars. Results indicated that exogenous plant growth regulators (PGRs) influenced RE, with kinetin and ZR inducing higher RE than thidiazuron (TDZ), though this was genotype dependent. Of the 6 cultivars screened for endogenous PGRs, the Tanzanian cultivar ‘Carrot’, which had with lowest RE, had the highest IAA:ZR ratio. Correlation between endogenous IAA and RE was strongly negative while weakly positive for ZR, which suggests that endogenous PGRs make a minor contribution to RE. In conclusion, 21% of cultivars with RE >40% were amenable to the 2-step protocol on application of exogenous PGRs; endogenous IAA and ZR levels are genotype-dependent, and higher endogenous IAA levels may contribute to inhibiting regeneration. For the remaining recalcitrant cultivars, we recommend further optimization, paying particular attention to endogenous PGRs that could have synergistic and antagonistic interactions with exogenous PGRs. 2022-04-25 2022-11-06T23:08:59Z 2022-11-06T23:08:59Z Journal Article https://hdl.handle.net/10568/125337 en Open Access International Society for Horticultural Science Wamalwa, L.; Tovar, J.; Indieka, S.; Torto, B.; Ghislain, M. 2022. Exogenous and endogenous plant growth regulators’ effect on regeneration of selected African sweetpotato (Ipomoea batatas L. Lam) cultivars. European Journal of Horticultural Science. ISSN 1611-4434. 87(2). 1-10. |
| spellingShingle | sweet potatoes organogenesis auxins cytokinins genetic improvement Wamalwa, L. Tovar, J.C. Indieka, S. Torto, B. Ghislain, M. Exogenous and endogenous plant growth regulators’ effect on regeneration of selected African sweetpotato (Ipomoea batatas L. Lam) cultivars |
| title | Exogenous and endogenous plant growth regulators’ effect on regeneration of selected African sweetpotato (Ipomoea batatas L. Lam) cultivars |
| title_full | Exogenous and endogenous plant growth regulators’ effect on regeneration of selected African sweetpotato (Ipomoea batatas L. Lam) cultivars |
| title_fullStr | Exogenous and endogenous plant growth regulators’ effect on regeneration of selected African sweetpotato (Ipomoea batatas L. Lam) cultivars |
| title_full_unstemmed | Exogenous and endogenous plant growth regulators’ effect on regeneration of selected African sweetpotato (Ipomoea batatas L. Lam) cultivars |
| title_short | Exogenous and endogenous plant growth regulators’ effect on regeneration of selected African sweetpotato (Ipomoea batatas L. Lam) cultivars |
| title_sort | exogenous and endogenous plant growth regulators effect on regeneration of selected african sweetpotato ipomoea batatas l lam cultivars |
| topic | sweet potatoes organogenesis auxins cytokinins genetic improvement |
| url | https://hdl.handle.net/10568/125337 |
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