Caracterización fenotípica y molecular de especies silvestres de solanum con resistencia cuantitativa al tizón tardio
Late blight caused by the oomycetous pathogen Phytophthora infestans (Mont.) de Bary is the most important disease of potato crop worldwide. The use of wild species as source of resistance in plant breeding programs has become a great alternative for the control of the disease. In order to identify...
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| Formato: | Tesis |
| Lenguaje: | Español |
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Universidad Nacional Agraria la Molina
2003
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| Acceso en línea: | https://hdl.handle.net/10568/125329 |
| _version_ | 1855518531805773824 |
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| author | Gonzales, E. |
| author_browse | Gonzales, E. |
| author_facet | Gonzales, E. |
| author_sort | Gonzales, E. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Late blight caused by the oomycetous pathogen Phytophthora infestans (Mont.) de Bary is the most important disease of potato crop worldwide. The use of wild species as source of resistance in plant breeding programs has become a great alternative for the control of the disease. In order to identify sources of quantitative resistance among wild Solanum species, 9 species (S. bukasovii, S. bulbocastanum, S. chomatophilum, S. capsicibaccatum, S. megistacrolobum, S. microdontum, S. raphanifolium, S. tarijense and S. verrucosum) were characterized for resistance to late blight in detached leaf assays, greenhouse and field experiments. Six of the selected species showed most of the genotypes free of R-genes, suggesting quantitative resistance to late blight. To identify the genes associated to quantitative resistance, two of these species ( S. megistacrolobum and S. raphanifolium) were characterized with molecular markers. The individuals of each species were grouped according to their phenotypic response in greenhouse in resistant or susceptible. These groups were then analyzed molecularly using ligation mediated polymerase chain reaction with primers obtained from genes involved in plant defense reactions. During this stage, new primers corresponding to genes that are suspected to have a role in plant defense were also designed. Then, the frequency of bands associated with each phenotypic group was tested using x2-test. Finally, some of the bands found significantly associated with one of the phenotypic categories were cloned and sequenced to verify if they correspond to candidate genes of the defense response. |
| format | Tesis |
| id | CGSpace125329 |
| institution | CGIAR Consortium |
| language | Español |
| publishDate | 2003 |
| publishDateRange | 2003 |
| publishDateSort | 2003 |
| publisher | Universidad Nacional Agraria la Molina |
| publisherStr | Universidad Nacional Agraria la Molina |
| record_format | dspace |
| spelling | CGSpace1253292025-11-06T14:17:44Z Caracterización fenotípica y molecular de especies silvestres de solanum con resistencia cuantitativa al tizón tardio Gonzales, E. solanum tuberosum potatoes genetics Late blight caused by the oomycetous pathogen Phytophthora infestans (Mont.) de Bary is the most important disease of potato crop worldwide. The use of wild species as source of resistance in plant breeding programs has become a great alternative for the control of the disease. In order to identify sources of quantitative resistance among wild Solanum species, 9 species (S. bukasovii, S. bulbocastanum, S. chomatophilum, S. capsicibaccatum, S. megistacrolobum, S. microdontum, S. raphanifolium, S. tarijense and S. verrucosum) were characterized for resistance to late blight in detached leaf assays, greenhouse and field experiments. Six of the selected species showed most of the genotypes free of R-genes, suggesting quantitative resistance to late blight. To identify the genes associated to quantitative resistance, two of these species ( S. megistacrolobum and S. raphanifolium) were characterized with molecular markers. The individuals of each species were grouped according to their phenotypic response in greenhouse in resistant or susceptible. These groups were then analyzed molecularly using ligation mediated polymerase chain reaction with primers obtained from genes involved in plant defense reactions. During this stage, new primers corresponding to genes that are suspected to have a role in plant defense were also designed. Then, the frequency of bands associated with each phenotypic group was tested using x2-test. Finally, some of the bands found significantly associated with one of the phenotypic categories were cloned and sequenced to verify if they correspond to candidate genes of the defense response. 2003 2022-11-03T21:17:24Z 2022-11-03T21:17:24Z Thesis https://hdl.handle.net/10568/125329 es Open Access application/pdf Universidad Nacional Agraria la Molina Gonzales, E. 2003. Caracterización fenotípica y molecular de especies silvestres de solanum con resistencia cuantitativa al tizón tardío. Universidad Nacional Agraria La Molina. Bachelor's thesis, Universidad Nacional Agraria La Molina. |
| spellingShingle | solanum tuberosum potatoes genetics Gonzales, E. Caracterización fenotípica y molecular de especies silvestres de solanum con resistencia cuantitativa al tizón tardio |
| title | Caracterización fenotípica y molecular de especies silvestres de solanum con resistencia cuantitativa al tizón tardio |
| title_full | Caracterización fenotípica y molecular de especies silvestres de solanum con resistencia cuantitativa al tizón tardio |
| title_fullStr | Caracterización fenotípica y molecular de especies silvestres de solanum con resistencia cuantitativa al tizón tardio |
| title_full_unstemmed | Caracterización fenotípica y molecular de especies silvestres de solanum con resistencia cuantitativa al tizón tardio |
| title_short | Caracterización fenotípica y molecular de especies silvestres de solanum con resistencia cuantitativa al tizón tardio |
| title_sort | caracterizacion fenotipica y molecular de especies silvestres de solanum con resistencia cuantitativa al tizon tardio |
| topic | solanum tuberosum potatoes genetics |
| url | https://hdl.handle.net/10568/125329 |
| work_keys_str_mv | AT gonzalese caracterizacionfenotipicaymoleculardeespeciessilvestresdesolanumconresistenciacuantitativaaltizontardio |