Mitochondrial genetic diversity of Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) associated with cassava in Lao PDR
Cassava Mosaic Disease (CMD) caused by Sri Lankan cassava mosaic virus (SLCMV), has rapidly spread in Southeast Asia (SEA) since 2016. Recently it has been documented in Lao PDR. Previous reports have identified whitefly species of B. tabaci as potential vectors of CMD in SEA, but their occurrence a...
| Main Authors: | , , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
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MDPI
2022
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/121932 |
| _version_ | 1855539065359695872 |
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| author | Leiva, Anne Chittarath, Khonesavanh López Alvarez, Diana Vongphachanh, Pinkham Gómez Jiménez, Maria Isabel Sengsay, Somkhit Wang, Xiao-Wei Rodríguez, Rafael Newby, Jonathan Craig Cuéllar, Wilmer Jose |
| author_browse | Chittarath, Khonesavanh Cuéllar, Wilmer Jose Gómez Jiménez, Maria Isabel Leiva, Anne López Alvarez, Diana Newby, Jonathan Craig Rodríguez, Rafael Sengsay, Somkhit Vongphachanh, Pinkham Wang, Xiao-Wei |
| author_facet | Leiva, Anne Chittarath, Khonesavanh López Alvarez, Diana Vongphachanh, Pinkham Gómez Jiménez, Maria Isabel Sengsay, Somkhit Wang, Xiao-Wei Rodríguez, Rafael Newby, Jonathan Craig Cuéllar, Wilmer Jose |
| author_sort | Leiva, Anne |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Cassava Mosaic Disease (CMD) caused by Sri Lankan cassava mosaic virus (SLCMV), has rapidly spread in Southeast Asia (SEA) since 2016. Recently it has been documented in Lao PDR. Previous reports have identified whitefly species of B. tabaci as potential vectors of CMD in SEA, but their occurrence and distribution in cassava fields is not well known. We conducted a countrywide survey in Lao PDR for adult whiteflies in cassava fields, and determined the abundance and genetic diversity of the B. tabaci species complex using mitochondrial cytochrome oxidase I (mtCOI) sequencing. In order to expedite the process, PCR amplifications were performed directly on whitefly adults without DNA extraction, and mtCOI sequences obtained using nanopore portable-sequencing technology. Low whitefly abundances and two cryptic species of the B. tabaci complex, Asia II 1 and Asia II 6, were identified. This is the first work on abundance and genetic identification of whiteflies associated with cassava in Lao PDR. This study indicates currently only a secondary role for Asia II in spreading CMD or as a pest. Routine monitoring and transmission studies on Asia II 6 should be carried out to establish its potential role as a vector of SLCMV in this region. |
| format | Journal Article |
| id | CGSpace121932 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | CGSpace1219322025-12-08T10:29:22Z Mitochondrial genetic diversity of Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) associated with cassava in Lao PDR Leiva, Anne Chittarath, Khonesavanh López Alvarez, Diana Vongphachanh, Pinkham Gómez Jiménez, Maria Isabel Sengsay, Somkhit Wang, Xiao-Wei Rodríguez, Rafael Newby, Jonathan Craig Cuéllar, Wilmer Jose bemisia tabaci plant viruses disease control mitochondrial genetics virus de las plantas control de enfermedades genética mitocondrial Cassava Mosaic Disease (CMD) caused by Sri Lankan cassava mosaic virus (SLCMV), has rapidly spread in Southeast Asia (SEA) since 2016. Recently it has been documented in Lao PDR. Previous reports have identified whitefly species of B. tabaci as potential vectors of CMD in SEA, but their occurrence and distribution in cassava fields is not well known. We conducted a countrywide survey in Lao PDR for adult whiteflies in cassava fields, and determined the abundance and genetic diversity of the B. tabaci species complex using mitochondrial cytochrome oxidase I (mtCOI) sequencing. In order to expedite the process, PCR amplifications were performed directly on whitefly adults without DNA extraction, and mtCOI sequences obtained using nanopore portable-sequencing technology. Low whitefly abundances and two cryptic species of the B. tabaci complex, Asia II 1 and Asia II 6, were identified. This is the first work on abundance and genetic identification of whiteflies associated with cassava in Lao PDR. This study indicates currently only a secondary role for Asia II in spreading CMD or as a pest. Routine monitoring and transmission studies on Asia II 6 should be carried out to establish its potential role as a vector of SLCMV in this region. 2022-09-22 2022-09-23T09:40:42Z 2022-09-23T09:40:42Z Journal Article https://hdl.handle.net/10568/121932 en Open Access application/pdf MDPI Leiva, A.; Chittarath, K.; Lopez-Alvarez, D.; Vongphachanh, P.; Gomez, M.; Sengsay, S.; Wang, X-W.; Rodriguez, R.; Newby, J.; Cuellar, W.J. (2022) Mitochondrial genetic diversity of Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) associated with cassava in Lao PDR. Insects 13: 861. 13 p. ISSN: 2075-4450 |
| spellingShingle | bemisia tabaci plant viruses disease control mitochondrial genetics virus de las plantas control de enfermedades genética mitocondrial Leiva, Anne Chittarath, Khonesavanh López Alvarez, Diana Vongphachanh, Pinkham Gómez Jiménez, Maria Isabel Sengsay, Somkhit Wang, Xiao-Wei Rodríguez, Rafael Newby, Jonathan Craig Cuéllar, Wilmer Jose Mitochondrial genetic diversity of Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) associated with cassava in Lao PDR |
| title | Mitochondrial genetic diversity of Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) associated with cassava in Lao PDR |
| title_full | Mitochondrial genetic diversity of Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) associated with cassava in Lao PDR |
| title_fullStr | Mitochondrial genetic diversity of Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) associated with cassava in Lao PDR |
| title_full_unstemmed | Mitochondrial genetic diversity of Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) associated with cassava in Lao PDR |
| title_short | Mitochondrial genetic diversity of Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) associated with cassava in Lao PDR |
| title_sort | mitochondrial genetic diversity of bemisia tabaci gennadius hemiptera aleyrodidae associated with cassava in lao pdr |
| topic | bemisia tabaci plant viruses disease control mitochondrial genetics virus de las plantas control de enfermedades genética mitocondrial |
| url | https://hdl.handle.net/10568/121932 |
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