The African swine fever isolate ASFV-Kenya-IX-1033 is highly virulent and stable after propagation in the wild boar cell line WSL
We describe the characterization of an African swine fever genotype IX virus (ASFV-Kenya-IX-1033), which was isolated from a domestic pig in western Kenya during a reported outbreak. This includes the efficiency of virus replication and in vivo virulence, together with genome stability and virulence...
| Autores principales: | , , , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
MDPI
2022
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/121075 |
| _version_ | 1855530621906976768 |
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| author | Hemmink, Johanneke D. Abkallo, Hussein M. Henson, Sonal P. Khazalwa, Emmanuel M. Oduor, Bernard Lacasta, Anna Okoth, Edward A. Riitho, Victor Fuchs, W. Bishop, Richard P. Steinaa, Lucilla |
| author_browse | Abkallo, Hussein M. Bishop, Richard P. Fuchs, W. Hemmink, Johanneke D. Henson, Sonal P. Khazalwa, Emmanuel M. Lacasta, Anna Oduor, Bernard Okoth, Edward A. Riitho, Victor Steinaa, Lucilla |
| author_facet | Hemmink, Johanneke D. Abkallo, Hussein M. Henson, Sonal P. Khazalwa, Emmanuel M. Oduor, Bernard Lacasta, Anna Okoth, Edward A. Riitho, Victor Fuchs, W. Bishop, Richard P. Steinaa, Lucilla |
| author_sort | Hemmink, Johanneke D. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | We describe the characterization of an African swine fever genotype IX virus (ASFV-Kenya-IX-1033), which was isolated from a domestic pig in western Kenya during a reported outbreak. This includes the efficiency of virus replication and in vivo virulence, together with genome stability and virulence, following passage in blood macrophages and in a wild boar lung cell line (WSL). The ASFV-Kenya-IX-1033 stock retained its ability to replicate in primary macrophages and retained virulence in vivo, following more than 20 passages in a WSL. At the whole genome level, a few single-nucleotide differences were observed between the macrophage and WSL-propagated viruses. Thus, we propose that the WSL is suitable for the production of live-attenuated ASFV vaccine candidates based on the modification of this wild-type isolate. The genome sequences for ASFV-Kenya-IX-1033 propagated in macrophages and in WSL cells were submitted to GenBank, and a challenge model based on the isolate was developed. This will aid the development of vaccines against the genotype IX ASFV circulating in eastern and central Africa. |
| format | Journal Article |
| id | CGSpace121075 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | CGSpace1210752025-12-08T10:29:22Z The African swine fever isolate ASFV-Kenya-IX-1033 is highly virulent and stable after propagation in the wild boar cell line WSL Hemmink, Johanneke D. Abkallo, Hussein M. Henson, Sonal P. Khazalwa, Emmanuel M. Oduor, Bernard Lacasta, Anna Okoth, Edward A. Riitho, Victor Fuchs, W. Bishop, Richard P. Steinaa, Lucilla african swine fever virus animal diseases swine We describe the characterization of an African swine fever genotype IX virus (ASFV-Kenya-IX-1033), which was isolated from a domestic pig in western Kenya during a reported outbreak. This includes the efficiency of virus replication and in vivo virulence, together with genome stability and virulence, following passage in blood macrophages and in a wild boar lung cell line (WSL). The ASFV-Kenya-IX-1033 stock retained its ability to replicate in primary macrophages and retained virulence in vivo, following more than 20 passages in a WSL. At the whole genome level, a few single-nucleotide differences were observed between the macrophage and WSL-propagated viruses. Thus, we propose that the WSL is suitable for the production of live-attenuated ASFV vaccine candidates based on the modification of this wild-type isolate. The genome sequences for ASFV-Kenya-IX-1033 propagated in macrophages and in WSL cells were submitted to GenBank, and a challenge model based on the isolate was developed. This will aid the development of vaccines against the genotype IX ASFV circulating in eastern and central Africa. 2022-08-29 2022-09-02T10:52:57Z 2022-09-02T10:52:57Z Journal Article https://hdl.handle.net/10568/121075 en Open Access MDPI Hemmink, J.D., Abkallo, H.M., Henson, S.P., Khazalwa, E.M., Oduor, B., Lacasta, A., Okoth, E., Riitho, V., Fuchs, W., Bishop, R.P. and Steinaa, L. 2022. The African swine fever isolate ASFV-Kenya-IX-1033 is highly virulent and stable after propagation in the wild boar cell line WSL. Viruses 14(9): 1912. |
| spellingShingle | african swine fever virus animal diseases swine Hemmink, Johanneke D. Abkallo, Hussein M. Henson, Sonal P. Khazalwa, Emmanuel M. Oduor, Bernard Lacasta, Anna Okoth, Edward A. Riitho, Victor Fuchs, W. Bishop, Richard P. Steinaa, Lucilla The African swine fever isolate ASFV-Kenya-IX-1033 is highly virulent and stable after propagation in the wild boar cell line WSL |
| title | The African swine fever isolate ASFV-Kenya-IX-1033 is highly virulent and stable after propagation in the wild boar cell line WSL |
| title_full | The African swine fever isolate ASFV-Kenya-IX-1033 is highly virulent and stable after propagation in the wild boar cell line WSL |
| title_fullStr | The African swine fever isolate ASFV-Kenya-IX-1033 is highly virulent and stable after propagation in the wild boar cell line WSL |
| title_full_unstemmed | The African swine fever isolate ASFV-Kenya-IX-1033 is highly virulent and stable after propagation in the wild boar cell line WSL |
| title_short | The African swine fever isolate ASFV-Kenya-IX-1033 is highly virulent and stable after propagation in the wild boar cell line WSL |
| title_sort | african swine fever isolate asfv kenya ix 1033 is highly virulent and stable after propagation in the wild boar cell line wsl |
| topic | african swine fever virus animal diseases swine |
| url | https://hdl.handle.net/10568/121075 |
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