Impact of orange essential oil on enteric methane emissions of heifers fed bermudagrass hay

In this study, the effects of orange essential oil (OEO) on the rumen fermentation, nutrient utilization, and methane (CH₄) emissions of beef heifers fed a diet of bermudagrass (Cynodon dactylon) were examined. In addition, in vitro and in situ experiments were conducted. The in vitro experiment con...

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Main Authors: Jiménez Ocampo, Rafael, Montoya Flores, María Denisse, Pamanes-Carrasco, Gerardo, Herrera-Torres, Esperanza, Arango, Jacobo, Estarrón-Esponosa, Mirna, Aguilar Pérez, Carlos Fernando, Araiza-Rosales, Elia E., Guerrero Cervantes, Maribel, Ku-Vera, Juan Carlos
Format: Journal Article
Language:Inglés
Published: Frontiers Media 2022
Subjects:
Online Access:https://hdl.handle.net/10568/121015
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author Jiménez Ocampo, Rafael
Montoya Flores, María Denisse
Pamanes-Carrasco, Gerardo
Herrera-Torres, Esperanza
Arango, Jacobo
Estarrón-Esponosa, Mirna
Aguilar Pérez, Carlos Fernando
Araiza-Rosales, Elia E.
Guerrero Cervantes, Maribel
Ku-Vera, Juan Carlos
author_browse Aguilar Pérez, Carlos Fernando
Araiza-Rosales, Elia E.
Arango, Jacobo
Estarrón-Esponosa, Mirna
Guerrero Cervantes, Maribel
Herrera-Torres, Esperanza
Jiménez Ocampo, Rafael
Ku-Vera, Juan Carlos
Montoya Flores, María Denisse
Pamanes-Carrasco, Gerardo
author_facet Jiménez Ocampo, Rafael
Montoya Flores, María Denisse
Pamanes-Carrasco, Gerardo
Herrera-Torres, Esperanza
Arango, Jacobo
Estarrón-Esponosa, Mirna
Aguilar Pérez, Carlos Fernando
Araiza-Rosales, Elia E.
Guerrero Cervantes, Maribel
Ku-Vera, Juan Carlos
author_sort Jiménez Ocampo, Rafael
collection Repository of Agricultural Research Outputs (CGSpace)
description In this study, the effects of orange essential oil (OEO) on the rumen fermentation, nutrient utilization, and methane (CH₄) emissions of beef heifers fed a diet of bermudagrass (Cynodon dactylon) were examined. In addition, in vitro and in situ experiments were conducted. The in vitro experiment consisted of three treatments: control (CTL, no OEO), OEO1 (0.25% OEO), and OEO2 (0.5% OEO). The forage to concentrate ratio was 70:30 (dry matter [DM] basis) in all treatments. No changes in pH, proportions of volatile fatty acids, and the acetate:propionate ratio were observed (P > 0.05). The addition of 0.25% OEO resulted in a reduction in CH₄ production (mL/g) relative to the control (P < 0.05). In the in situ experiment, 5 g of total mixed ration (CTL, OEO1, and OEO2) were incubated for 6, 12, 24, 48, and 72 h. Potential and effective degradability were not affected by OEO supplementation (P > 0.05). In the in vivo study, six crossbred beef heifers (Bos indicus × Bos taurus), fitted with rumen cannulas, were assigned to three different treatments: no additive (CTL), 0.25% OEO (OEO1), and 0.5% OEO (OEO2) in a replicated 3 × 3 Latin square (21-day periods). Heifers were fed at 2.8% body weight. In vivo CH₄ production was measured in open-circuit respiration chambers. Reductions in gross energy consumption, apparent total tract digestibility, and rumen valerate concentration were observed for OEO2 compared to the control (P < 0.05). Additionally, decreases in CH₄ emissions (g/day; P < 0.05) and CH4 (MJ gross energy intake/day; P < 0.05) were observed in response to supplementation of 0.5% OEO as compared to the CTL treatment. Thus, supplementation of 0.5% OEO reduced CH₄ emissions (g/day) by 12% without impacting the DM intake of heifers fed bermudagrass hay as a basal ration.
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spelling CGSpace1210152025-12-08T10:29:22Z Impact of orange essential oil on enteric methane emissions of heifers fed bermudagrass hay Jiménez Ocampo, Rafael Montoya Flores, María Denisse Pamanes-Carrasco, Gerardo Herrera-Torres, Esperanza Arango, Jacobo Estarrón-Esponosa, Mirna Aguilar Pérez, Carlos Fernando Araiza-Rosales, Elia E. Guerrero Cervantes, Maribel Ku-Vera, Juan Carlos cattle methane essential oils feed additives metabolites ganado bovino metano aceites esenciales aditivos de piensos In this study, the effects of orange essential oil (OEO) on the rumen fermentation, nutrient utilization, and methane (CH₄) emissions of beef heifers fed a diet of bermudagrass (Cynodon dactylon) were examined. In addition, in vitro and in situ experiments were conducted. The in vitro experiment consisted of three treatments: control (CTL, no OEO), OEO1 (0.25% OEO), and OEO2 (0.5% OEO). The forage to concentrate ratio was 70:30 (dry matter [DM] basis) in all treatments. No changes in pH, proportions of volatile fatty acids, and the acetate:propionate ratio were observed (P > 0.05). The addition of 0.25% OEO resulted in a reduction in CH₄ production (mL/g) relative to the control (P < 0.05). In the in situ experiment, 5 g of total mixed ration (CTL, OEO1, and OEO2) were incubated for 6, 12, 24, 48, and 72 h. Potential and effective degradability were not affected by OEO supplementation (P > 0.05). In the in vivo study, six crossbred beef heifers (Bos indicus × Bos taurus), fitted with rumen cannulas, were assigned to three different treatments: no additive (CTL), 0.25% OEO (OEO1), and 0.5% OEO (OEO2) in a replicated 3 × 3 Latin square (21-day periods). Heifers were fed at 2.8% body weight. In vivo CH₄ production was measured in open-circuit respiration chambers. Reductions in gross energy consumption, apparent total tract digestibility, and rumen valerate concentration were observed for OEO2 compared to the control (P < 0.05). Additionally, decreases in CH₄ emissions (g/day; P < 0.05) and CH4 (MJ gross energy intake/day; P < 0.05) were observed in response to supplementation of 0.5% OEO as compared to the CTL treatment. Thus, supplementation of 0.5% OEO reduced CH₄ emissions (g/day) by 12% without impacting the DM intake of heifers fed bermudagrass hay as a basal ration. 2022-08-16 2022-08-30T12:35:30Z 2022-08-30T12:35:30Z Journal Article https://hdl.handle.net/10568/121015 en Open Access application/pdf Frontiers Media Jiménez-Ocampo, R.; Montoya-Flores, M.D.; Pamanes-Carrasco, G.; Herrera-Torres, E.; Arango, J.; Estarrón-Esponosa, M.; Aguilar-Pérez, C.F.; Araiza-Rosales, E.E.; Guerrero-Cervantes, M.; Ku-Vera, J.C. (2022) Impact of orange essential oil on enteric methane emissions of heifers fed bermudagrass hay. Frontiers in Veterinary Science 9:863910. ISSN: 2297-1769
spellingShingle cattle
methane
essential oils
feed additives
metabolites
ganado bovino
metano
aceites esenciales
aditivos de piensos
Jiménez Ocampo, Rafael
Montoya Flores, María Denisse
Pamanes-Carrasco, Gerardo
Herrera-Torres, Esperanza
Arango, Jacobo
Estarrón-Esponosa, Mirna
Aguilar Pérez, Carlos Fernando
Araiza-Rosales, Elia E.
Guerrero Cervantes, Maribel
Ku-Vera, Juan Carlos
Impact of orange essential oil on enteric methane emissions of heifers fed bermudagrass hay
title Impact of orange essential oil on enteric methane emissions of heifers fed bermudagrass hay
title_full Impact of orange essential oil on enteric methane emissions of heifers fed bermudagrass hay
title_fullStr Impact of orange essential oil on enteric methane emissions of heifers fed bermudagrass hay
title_full_unstemmed Impact of orange essential oil on enteric methane emissions of heifers fed bermudagrass hay
title_short Impact of orange essential oil on enteric methane emissions of heifers fed bermudagrass hay
title_sort impact of orange essential oil on enteric methane emissions of heifers fed bermudagrass hay
topic cattle
methane
essential oils
feed additives
metabolites
ganado bovino
metano
aceites esenciales
aditivos de piensos
url https://hdl.handle.net/10568/121015
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