Transcriptional organization of the Salmonella Typhimurium phage P22 pid ORFan locus
Many phage genes lack sequence similarity to any other open reading frame (ORF) in current databases. These enigmatic ORFan genes can have a tremendous impact on phage propagation and host interactions but often remain experimentally unexplored. We previously revealed a novel interaction between pha...
| Main Authors: | , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
MDPI
2022
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/121009 |
| _version_ | 1855524387982147584 |
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| author | Wolput, S. Makumi, Angela Wicke, L. Bäcker, L.E. Cenens, W. Briers, Y. Wenner, N.A. Owen, S.V. Hinton, J.C.D. Lavigne, R. Aertsen, A. |
| author_browse | Aertsen, A. Briers, Y. Bäcker, L.E. Cenens, W. Hinton, J.C.D. Lavigne, R. Makumi, Angela Owen, S.V. Wenner, N.A. Wicke, L. Wolput, S. |
| author_facet | Wolput, S. Makumi, Angela Wicke, L. Bäcker, L.E. Cenens, W. Briers, Y. Wenner, N.A. Owen, S.V. Hinton, J.C.D. Lavigne, R. Aertsen, A. |
| author_sort | Wolput, S. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Many phage genes lack sequence similarity to any other open reading frame (ORF) in current databases. These enigmatic ORFan genes can have a tremendous impact on phage propagation and host interactions but often remain experimentally unexplored. We previously revealed a novel interaction between phage P22 and its Salmonella Typhimurium host, instigated by the ORFan gene pid (for phage P22 encoded instigator of dgo expression) and resulting in derepression of the host dgoRKAT operon. The pid gene is highly expressed in phage carrier cells that harbor a polarly located P22 episome that segregates asymmetrically among daughter cells. Here, we discovered that the pid locus is fitted with a weak promoter, has an exceptionally long 5′ untranslated region that is instructive for a secondary pid mRNA species, and has a 3′ Rho-independent termination loop that is responsible for stability of the pid transcript. |
| format | Journal Article |
| id | CGSpace121009 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | CGSpace1210092025-12-08T10:29:22Z Transcriptional organization of the Salmonella Typhimurium phage P22 pid ORFan locus Wolput, S. Makumi, Angela Wicke, L. Bäcker, L.E. Cenens, W. Briers, Y. Wenner, N.A. Owen, S.V. Hinton, J.C.D. Lavigne, R. Aertsen, A. bacteriophages research Many phage genes lack sequence similarity to any other open reading frame (ORF) in current databases. These enigmatic ORFan genes can have a tremendous impact on phage propagation and host interactions but often remain experimentally unexplored. We previously revealed a novel interaction between phage P22 and its Salmonella Typhimurium host, instigated by the ORFan gene pid (for phage P22 encoded instigator of dgo expression) and resulting in derepression of the host dgoRKAT operon. The pid gene is highly expressed in phage carrier cells that harbor a polarly located P22 episome that segregates asymmetrically among daughter cells. Here, we discovered that the pid locus is fitted with a weak promoter, has an exceptionally long 5′ untranslated region that is instructive for a secondary pid mRNA species, and has a 3′ Rho-independent termination loop that is responsible for stability of the pid transcript. 2022-02-02 2022-08-30T10:22:29Z 2022-08-30T10:22:29Z Journal Article https://hdl.handle.net/10568/121009 en Open Access MDPI Wolput, S., Makumi, A., Wicke, L., Bäcker, L.E., Cenens, W., Briers, Y., Wenner, N.A., Owen, S.V., Hinton, J.C.D., Lavigne, R. and Aertsen, A. 2022. Transcriptional organization of the Salmonella Typhimurium phage P22 pid ORFan locus. International Journal of Molecular Sciences 23(3): 1253. |
| spellingShingle | bacteriophages research Wolput, S. Makumi, Angela Wicke, L. Bäcker, L.E. Cenens, W. Briers, Y. Wenner, N.A. Owen, S.V. Hinton, J.C.D. Lavigne, R. Aertsen, A. Transcriptional organization of the Salmonella Typhimurium phage P22 pid ORFan locus |
| title | Transcriptional organization of the Salmonella Typhimurium phage P22 pid ORFan locus |
| title_full | Transcriptional organization of the Salmonella Typhimurium phage P22 pid ORFan locus |
| title_fullStr | Transcriptional organization of the Salmonella Typhimurium phage P22 pid ORFan locus |
| title_full_unstemmed | Transcriptional organization of the Salmonella Typhimurium phage P22 pid ORFan locus |
| title_short | Transcriptional organization of the Salmonella Typhimurium phage P22 pid ORFan locus |
| title_sort | transcriptional organization of the salmonella typhimurium phage p22 pid orfan locus |
| topic | bacteriophages research |
| url | https://hdl.handle.net/10568/121009 |
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