An optimized nucleic acid isolation protocol for virus diagnostics in cassava (Manihot esculenta Crantz.)
Our group works on the detection and characterization of cassava viruses, supporting projects that involve large scale pathogen surveillance activities and resistance screening assays in multiple and remote locations. In order to comply with these applications, nucleic acid isolation protocols need...
| Autores principales: | , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2021
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/120937 |
| _version_ | 1855537799349927936 |
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| author | Jiménez Polo, Jenyfer Leiva Sandoval, Ana Maria Olaya, Cristian A. Acosta-Trujillo, Daniela Cuéllar, Wilmer Jose |
| author_browse | Acosta-Trujillo, Daniela Cuéllar, Wilmer Jose Jiménez Polo, Jenyfer Leiva Sandoval, Ana Maria Olaya, Cristian A. |
| author_facet | Jiménez Polo, Jenyfer Leiva Sandoval, Ana Maria Olaya, Cristian A. Acosta-Trujillo, Daniela Cuéllar, Wilmer Jose |
| author_sort | Jiménez Polo, Jenyfer |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Our group works on the detection and characterization of cassava viruses, supporting projects that involve large scale pathogen surveillance activities and resistance screening assays in multiple and remote locations. In order to comply with these applications, nucleic acid isolation protocols need to be cost effective, adjusted for samples that will stand long distance transport and harsh storage conditions, while maximizing the yield and quality of the nucleic acid extracts obtained. The method we describe here has been widely used and validated using different downstream tests (including, but not limited to, Rolling Circle Amplification and Illumina and Nanopore sequencing), but is currently unpublished. The protocol begins with milligram amounts of dry leaf samples stored in silica gel, does not require liquid Nitrogen nor phenol extraction and produces an average of 2.11 µg of nucleic acids per mg of dry tissue. |
| format | Journal Article |
| id | CGSpace120937 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | CGSpace1209372025-11-11T19:04:22Z An optimized nucleic acid isolation protocol for virus diagnostics in cassava (Manihot esculenta Crantz.) Jiménez Polo, Jenyfer Leiva Sandoval, Ana Maria Olaya, Cristian A. Acosta-Trujillo, Daniela Cuéllar, Wilmer Jose nucleic acids diagnostic techniques methods acidos nucléicos técnicas de diagnosis métodos Our group works on the detection and characterization of cassava viruses, supporting projects that involve large scale pathogen surveillance activities and resistance screening assays in multiple and remote locations. In order to comply with these applications, nucleic acid isolation protocols need to be cost effective, adjusted for samples that will stand long distance transport and harsh storage conditions, while maximizing the yield and quality of the nucleic acid extracts obtained. The method we describe here has been widely used and validated using different downstream tests (including, but not limited to, Rolling Circle Amplification and Illumina and Nanopore sequencing), but is currently unpublished. The protocol begins with milligram amounts of dry leaf samples stored in silica gel, does not require liquid Nitrogen nor phenol extraction and produces an average of 2.11 µg of nucleic acids per mg of dry tissue. 2021 2022-08-25T07:39:11Z 2022-08-25T07:39:11Z Journal Article https://hdl.handle.net/10568/120937 en Open Access application/pdf Elsevier Jimenez, J.; Leiva, A.M.; Olaya, C.; Acosta-Trujillo, D.; Cuellar, W. (2021) An optimized nucleic acid isolation protocol for virus diagnostics in cassava (Manihot esculenta Crantz). MethodsX 8: 101496. 7 p. ISSN: 2215-0161 |
| spellingShingle | nucleic acids diagnostic techniques methods acidos nucléicos técnicas de diagnosis métodos Jiménez Polo, Jenyfer Leiva Sandoval, Ana Maria Olaya, Cristian A. Acosta-Trujillo, Daniela Cuéllar, Wilmer Jose An optimized nucleic acid isolation protocol for virus diagnostics in cassava (Manihot esculenta Crantz.) |
| title | An optimized nucleic acid isolation protocol for virus diagnostics in cassava (Manihot esculenta Crantz.) |
| title_full | An optimized nucleic acid isolation protocol for virus diagnostics in cassava (Manihot esculenta Crantz.) |
| title_fullStr | An optimized nucleic acid isolation protocol for virus diagnostics in cassava (Manihot esculenta Crantz.) |
| title_full_unstemmed | An optimized nucleic acid isolation protocol for virus diagnostics in cassava (Manihot esculenta Crantz.) |
| title_short | An optimized nucleic acid isolation protocol for virus diagnostics in cassava (Manihot esculenta Crantz.) |
| title_sort | optimized nucleic acid isolation protocol for virus diagnostics in cassava manihot esculenta crantz |
| topic | nucleic acids diagnostic techniques methods acidos nucléicos técnicas de diagnosis métodos |
| url | https://hdl.handle.net/10568/120937 |
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