Incidence of RNA viruses infecting taro and tannia in East Africa and molecular characterisation of dasheen mosaic virusisolates
Taro (Colocasia esculenta) and tannia (Xanthosoma sp.) plants growing in 25 districts across Ethiopia, Kenya, Tanzania and Uganda were surveyed for four RNA viruses. Leaf samples from 392 plants were tested for cucumber mosaic virus (CMV), dasheen mosaic virus (DsMV), taro vein chlorosis virus (TaVC...
| Autores principales: | , , , , , , , |
|---|---|
| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Wiley
2022
|
| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/120387 |
| _version_ | 1855531244947767296 |
|---|---|
| author | Kidanemariam, Dawit B. Sukal, A.C. Abraham, Adane D. Njuguna, Joyce N. Stomeo, Francesca Dale, J.L. James, A.P. Harding, R.M. |
| author_browse | Abraham, Adane D. Dale, J.L. Harding, R.M. James, A.P. Kidanemariam, Dawit B. Njuguna, Joyce N. Stomeo, Francesca Sukal, A.C. |
| author_facet | Kidanemariam, Dawit B. Sukal, A.C. Abraham, Adane D. Njuguna, Joyce N. Stomeo, Francesca Dale, J.L. James, A.P. Harding, R.M. |
| author_sort | Kidanemariam, Dawit B. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Taro (Colocasia esculenta) and tannia (Xanthosoma sp.) plants growing in 25 districts across Ethiopia, Kenya, Tanzania and Uganda were surveyed for four RNA viruses. Leaf samples from 392 plants were tested for cucumber mosaic virus (CMV), dasheen mosaic virus (DsMV), taro vein chlorosis virus (TaVCV) and Colocasia bobone disease‐associated virus (CBDaV) by RT‐PCR. No samples tested positive for TaVCV or CBDaV, while CMV was only detected in three tannia samples with mosaic symptoms from Uganda. DsMV was detected in 40 samples, including 36 out of 171 from Ethiopia, one out of 94 from Uganda and three out of 41 from Tanzania, while none of the 86 samples from Kenya tested positive for any of the four viruses. The complete genomes of nine DsMV isolates from East Africa were cloned and sequenced. Phylogenetic analyses based on the amino acid sequence of the DsMV CP‐coding region revealed two distinct clades. Isolates from Ethiopia were distributed in both clades, while samples from Uganda and Tanzania belong to different clades. Seven possible recombination events were identified from the analysis carried out on the available 15 full‐length DsMV isolates. Nucleotide substitution ratio analysis revealed that all the DsMV genes are under strong negative selection pressure. |
| format | Journal Article |
| id | CGSpace120387 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | CGSpace1203872024-03-06T10:16:43Z Incidence of RNA viruses infecting taro and tannia in East Africa and molecular characterisation of dasheen mosaic virusisolates Kidanemariam, Dawit B. Sukal, A.C. Abraham, Adane D. Njuguna, Joyce N. Stomeo, Francesca Dale, J.L. James, A.P. Harding, R.M. viruses taro xanthosoma sagittifolium plant viruses colocasia esculenta Taro (Colocasia esculenta) and tannia (Xanthosoma sp.) plants growing in 25 districts across Ethiopia, Kenya, Tanzania and Uganda were surveyed for four RNA viruses. Leaf samples from 392 plants were tested for cucumber mosaic virus (CMV), dasheen mosaic virus (DsMV), taro vein chlorosis virus (TaVCV) and Colocasia bobone disease‐associated virus (CBDaV) by RT‐PCR. No samples tested positive for TaVCV or CBDaV, while CMV was only detected in three tannia samples with mosaic symptoms from Uganda. DsMV was detected in 40 samples, including 36 out of 171 from Ethiopia, one out of 94 from Uganda and three out of 41 from Tanzania, while none of the 86 samples from Kenya tested positive for any of the four viruses. The complete genomes of nine DsMV isolates from East Africa were cloned and sequenced. Phylogenetic analyses based on the amino acid sequence of the DsMV CP‐coding region revealed two distinct clades. Isolates from Ethiopia were distributed in both clades, while samples from Uganda and Tanzania belong to different clades. Seven possible recombination events were identified from the analysis carried out on the available 15 full‐length DsMV isolates. Nucleotide substitution ratio analysis revealed that all the DsMV genes are under strong negative selection pressure. 2022-03 2022-08-01T14:33:26Z 2022-08-01T14:33:26Z Journal Article https://hdl.handle.net/10568/120387 en Open Access Wiley Kidanemariam, D.B., Sukal, A.C., Abraham, A.D., Njuguna, J.N., Stomeo, F., Dale, J.L., James, A.P. and Harding, R.M. 2022. Incidence of RNA viruses infecting taro and tannia in East Africa and molecular characterisation of dasheen mosaic virusisolates. Annals of Applied Biology 180(2): 211–223. |
| spellingShingle | viruses taro xanthosoma sagittifolium plant viruses colocasia esculenta Kidanemariam, Dawit B. Sukal, A.C. Abraham, Adane D. Njuguna, Joyce N. Stomeo, Francesca Dale, J.L. James, A.P. Harding, R.M. Incidence of RNA viruses infecting taro and tannia in East Africa and molecular characterisation of dasheen mosaic virusisolates |
| title | Incidence of RNA viruses infecting taro and tannia in East Africa and molecular characterisation of dasheen mosaic virusisolates |
| title_full | Incidence of RNA viruses infecting taro and tannia in East Africa and molecular characterisation of dasheen mosaic virusisolates |
| title_fullStr | Incidence of RNA viruses infecting taro and tannia in East Africa and molecular characterisation of dasheen mosaic virusisolates |
| title_full_unstemmed | Incidence of RNA viruses infecting taro and tannia in East Africa and molecular characterisation of dasheen mosaic virusisolates |
| title_short | Incidence of RNA viruses infecting taro and tannia in East Africa and molecular characterisation of dasheen mosaic virusisolates |
| title_sort | incidence of rna viruses infecting taro and tannia in east africa and molecular characterisation of dasheen mosaic virusisolates |
| topic | viruses taro xanthosoma sagittifolium plant viruses colocasia esculenta |
| url | https://hdl.handle.net/10568/120387 |
| work_keys_str_mv | AT kidanemariamdawitb incidenceofrnavirusesinfectingtaroandtanniaineastafricaandmolecularcharacterisationofdasheenmosaicvirusisolates AT sukalac incidenceofrnavirusesinfectingtaroandtanniaineastafricaandmolecularcharacterisationofdasheenmosaicvirusisolates AT abrahamadaned incidenceofrnavirusesinfectingtaroandtanniaineastafricaandmolecularcharacterisationofdasheenmosaicvirusisolates AT njugunajoycen incidenceofrnavirusesinfectingtaroandtanniaineastafricaandmolecularcharacterisationofdasheenmosaicvirusisolates AT stomeofrancesca incidenceofrnavirusesinfectingtaroandtanniaineastafricaandmolecularcharacterisationofdasheenmosaicvirusisolates AT dalejl incidenceofrnavirusesinfectingtaroandtanniaineastafricaandmolecularcharacterisationofdasheenmosaicvirusisolates AT jamesap incidenceofrnavirusesinfectingtaroandtanniaineastafricaandmolecularcharacterisationofdasheenmosaicvirusisolates AT hardingrm incidenceofrnavirusesinfectingtaroandtanniaineastafricaandmolecularcharacterisationofdasheenmosaicvirusisolates |