Molecular Characterization and DNA Fingerprinting of Xanthomonas oryzae pv. oryzae Isolates from Climate Change Prone Areas in East Africa
Genomic DNA fingerprinting is a useful tool for effective and reliable identification and differentiation of Xanthomonas oryzae pv. oryzae (Xoo) pathogen from rice. The study aimed to conduct molecular characterization and DNA fingerprinting of 23 Xoo isolates from East Africa and two Xoo isolates f...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
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Science Alert
2011
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/120195 |
| _version_ | 1855529057517568000 |
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| author | Onasanya, A. Kiepe, P. Basso, A. Nkima, G. Nwilene, F.E. Ingelbrecht, I.L. Lamo, J. Ekperigin, M.M. Onasanya, R.O. Oyelakin, O. Winter, S. Sere, Y. |
| author_browse | Basso, A. Ekperigin, M.M. Ingelbrecht, I.L. Kiepe, P. Lamo, J. Nkima, G. Nwilene, F.E. Onasanya, A. Onasanya, R.O. Oyelakin, O. Sere, Y. Winter, S. |
| author_facet | Onasanya, A. Kiepe, P. Basso, A. Nkima, G. Nwilene, F.E. Ingelbrecht, I.L. Lamo, J. Ekperigin, M.M. Onasanya, R.O. Oyelakin, O. Winter, S. Sere, Y. |
| author_sort | Onasanya, A. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Genomic DNA fingerprinting is a useful tool for effective and reliable identification and differentiation of Xanthomonas oryzae pv. oryzae (Xoo) pathogen from rice. The study aimed to conduct molecular characterization and DNA fingerprinting of 23 Xoo isolates from East Africa and two Xoo isolates from IRRI (Philippines) as control. PCR analysis was carryout on genomic DNA of 25 Xoo isolates using 6 Xoo specific primer pairs. Cluster analyses of genetic data obtained from 25 Xoo DNA fingerprints revealed two major genotypes (GrpA and GrpB) among the 25 Xoo isolates. GrpA has three subgroups (GrpA1; GrpA2; GrpA3) and GrpB (GrpB1; GrpB2; GrpB3). GrpA genotype consists of 20 Xoo isolates from Uganda, Rwanda and Philippines while GrpB genotype has 5 Xoo isolates from Rwanda. Some Xoo isolates were identical (PX-1, PX-2; UX621, RX2101; RX554, UX623, RX4113; UX211, UX213, UX214, RX4112, UX215). The emergence of subgroup genotypes could possibly be due to mutations and interactions among isolates and strains in host cells. Some Xoo isolates from Rwanda and Uganda were identical suggesting possible pathogen migration between these countries and long-term survival. Durable resistance rice cultivars would need to overcome both GrpA and GrpB Xoo genotypes in order to survive after their deployment into different rice ecologies in East Africa |
| format | Journal Article |
| id | CGSpace120195 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2011 |
| publishDateRange | 2011 |
| publishDateSort | 2011 |
| publisher | Science Alert |
| publisherStr | Science Alert |
| record_format | dspace |
| spelling | CGSpace1201952025-12-08T09:54:28Z Molecular Characterization and DNA Fingerprinting of Xanthomonas oryzae pv. oryzae Isolates from Climate Change Prone Areas in East Africa Onasanya, A. Kiepe, P. Basso, A. Nkima, G. Nwilene, F.E. Ingelbrecht, I.L. Lamo, J. Ekperigin, M.M. Onasanya, R.O. Oyelakin, O. Winter, S. Sere, Y. xanthomonas oryzae pv. oryzicola dna fingerprinting east africa Genomic DNA fingerprinting is a useful tool for effective and reliable identification and differentiation of Xanthomonas oryzae pv. oryzae (Xoo) pathogen from rice. The study aimed to conduct molecular characterization and DNA fingerprinting of 23 Xoo isolates from East Africa and two Xoo isolates from IRRI (Philippines) as control. PCR analysis was carryout on genomic DNA of 25 Xoo isolates using 6 Xoo specific primer pairs. Cluster analyses of genetic data obtained from 25 Xoo DNA fingerprints revealed two major genotypes (GrpA and GrpB) among the 25 Xoo isolates. GrpA has three subgroups (GrpA1; GrpA2; GrpA3) and GrpB (GrpB1; GrpB2; GrpB3). GrpA genotype consists of 20 Xoo isolates from Uganda, Rwanda and Philippines while GrpB genotype has 5 Xoo isolates from Rwanda. Some Xoo isolates were identical (PX-1, PX-2; UX621, RX2101; RX554, UX623, RX4113; UX211, UX213, UX214, RX4112, UX215). The emergence of subgroup genotypes could possibly be due to mutations and interactions among isolates and strains in host cells. Some Xoo isolates from Rwanda and Uganda were identical suggesting possible pathogen migration between these countries and long-term survival. Durable resistance rice cultivars would need to overcome both GrpA and GrpB Xoo genotypes in order to survive after their deployment into different rice ecologies in East Africa 2011-09-01 2022-07-20T06:45:30Z 2022-07-20T06:45:30Z Journal Article https://hdl.handle.net/10568/120195 en Open Access Science Alert Onasanya, A. Kiepe, P. Basso, A. Nkima, G. Nwilene, F.E. Ingelbrecht, I. Lamo, J. Ekperigin, M.M. Onasanya, R.O. Oyelakin, O. Winter, S. Sere, Y. Molecular Characterization and DNA Fingerprinting of Xanthomonas oryzae pv. oryzae Isolates from Climate Change Prone Areas in East Africa. Research Journal of Environmental Sciences. 2011, Volume: 5, Issue 9: 772-778. |
| spellingShingle | xanthomonas oryzae pv. oryzicola dna fingerprinting east africa Onasanya, A. Kiepe, P. Basso, A. Nkima, G. Nwilene, F.E. Ingelbrecht, I.L. Lamo, J. Ekperigin, M.M. Onasanya, R.O. Oyelakin, O. Winter, S. Sere, Y. Molecular Characterization and DNA Fingerprinting of Xanthomonas oryzae pv. oryzae Isolates from Climate Change Prone Areas in East Africa |
| title | Molecular Characterization and DNA Fingerprinting of Xanthomonas oryzae pv. oryzae Isolates from Climate Change Prone Areas in East Africa |
| title_full | Molecular Characterization and DNA Fingerprinting of Xanthomonas oryzae pv. oryzae Isolates from Climate Change Prone Areas in East Africa |
| title_fullStr | Molecular Characterization and DNA Fingerprinting of Xanthomonas oryzae pv. oryzae Isolates from Climate Change Prone Areas in East Africa |
| title_full_unstemmed | Molecular Characterization and DNA Fingerprinting of Xanthomonas oryzae pv. oryzae Isolates from Climate Change Prone Areas in East Africa |
| title_short | Molecular Characterization and DNA Fingerprinting of Xanthomonas oryzae pv. oryzae Isolates from Climate Change Prone Areas in East Africa |
| title_sort | molecular characterization and dna fingerprinting of xanthomonas oryzae pv oryzae isolates from climate change prone areas in east africa |
| topic | xanthomonas oryzae pv. oryzicola dna fingerprinting east africa |
| url | https://hdl.handle.net/10568/120195 |
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