Genetic Fingerprinting of Pseudomonas aeruginosa Involved in Nosocomial Infection as Revealed by RAPD-PCR Markers
Genetic fingerprinting of 30 Pseudomonas aeruginosa (Pa) isolates from three types of nosocomial infection cases from two Osun State Teaching Hospitals was compared using RAPD-PCR markers. Ten out of 50 operon primers tested showed polymorphism with reproducible results among the isolates and produc...
| Autores principales: | , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Science Alert
2010
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/120190 |
| _version_ | 1855519141091344384 |
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| author | Akanji, B.O. Ajele, J.O. Onasanya, A. Oyelakin, O. |
| author_browse | Ajele, J.O. Akanji, B.O. Onasanya, A. Oyelakin, O. |
| author_facet | Akanji, B.O. Ajele, J.O. Onasanya, A. Oyelakin, O. |
| author_sort | Akanji, B.O. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Genetic fingerprinting of 30 Pseudomonas aeruginosa (Pa) isolates from three types of nosocomial infection cases from two Osun State Teaching Hospitals was compared using RAPD-PCR markers. Ten out of 50 operon primers tested showed polymorphism with reproducible results among the isolates and produced 131 bands of which 74 were polymorphic with sizes ranging between 200 and 3,000 bp. Cluster analysis using the 74 polymorphic markers classified the 30 Pseudomonas aeruginosa isolates into two (PgA and PgB) genetic groups. Comparing isolates proportion in each genotype based on their site of infection, antibiotics resistance pattern and geographical location, it was revealed that the proportion of urinary tract infection isolates in PgA genotype was significantly less than those in PgB genotype (z = -1.195, p<0.05) while the proportion of septicaemia isolates in PgA genotype was significantly higher than its proportion in PgB genotype (z = 1.348, p<0.05). However the proportion of wound infection isolates of PgA and PgB genotypes were significantly the same (z = -0.278, p>0.05). The PgA genotype contained few isolates with increased virulence and resistance to new antimicrobial modules and could possibly be new emerging P. aeruginosa strains from PgB genotype population. The study has critically revealed the genetic diversity and distribution among P. aeruginosa isolates in Osun State |
| format | Journal Article |
| id | CGSpace120190 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2010 |
| publishDateRange | 2010 |
| publishDateSort | 2010 |
| publisher | Science Alert |
| publisherStr | Science Alert |
| record_format | dspace |
| spelling | CGSpace1201902025-12-08T09:54:28Z Genetic Fingerprinting of Pseudomonas aeruginosa Involved in Nosocomial Infection as Revealed by RAPD-PCR Markers Akanji, B.O. Ajele, J.O. Onasanya, A. Oyelakin, O. pseudomonas aeruginosa polymorphism genotype virulence antibiotic resistance Genetic fingerprinting of 30 Pseudomonas aeruginosa (Pa) isolates from three types of nosocomial infection cases from two Osun State Teaching Hospitals was compared using RAPD-PCR markers. Ten out of 50 operon primers tested showed polymorphism with reproducible results among the isolates and produced 131 bands of which 74 were polymorphic with sizes ranging between 200 and 3,000 bp. Cluster analysis using the 74 polymorphic markers classified the 30 Pseudomonas aeruginosa isolates into two (PgA and PgB) genetic groups. Comparing isolates proportion in each genotype based on their site of infection, antibiotics resistance pattern and geographical location, it was revealed that the proportion of urinary tract infection isolates in PgA genotype was significantly less than those in PgB genotype (z = -1.195, p<0.05) while the proportion of septicaemia isolates in PgA genotype was significantly higher than its proportion in PgB genotype (z = 1.348, p<0.05). However the proportion of wound infection isolates of PgA and PgB genotypes were significantly the same (z = -0.278, p>0.05). The PgA genotype contained few isolates with increased virulence and resistance to new antimicrobial modules and could possibly be new emerging P. aeruginosa strains from PgB genotype population. The study has critically revealed the genetic diversity and distribution among P. aeruginosa isolates in Osun State 2010-12-15 2022-07-20T06:45:28Z 2022-07-20T06:45:28Z Journal Article https://hdl.handle.net/10568/120190 en Open Access Science Alert Akanji, B.O. Ajele, J.O. Onasanya, A. Oyelakin, O. Genetic Fingerprinting of Pseudomonas aeruginosa Involved in Nosocomial Infection as Revealed by RAPD-PCR Markers.Biotechnology. 2011, Volume 10, Issue 1: 70-77. |
| spellingShingle | pseudomonas aeruginosa polymorphism genotype virulence antibiotic resistance Akanji, B.O. Ajele, J.O. Onasanya, A. Oyelakin, O. Genetic Fingerprinting of Pseudomonas aeruginosa Involved in Nosocomial Infection as Revealed by RAPD-PCR Markers |
| title | Genetic Fingerprinting of Pseudomonas aeruginosa Involved in Nosocomial Infection as Revealed by RAPD-PCR Markers |
| title_full | Genetic Fingerprinting of Pseudomonas aeruginosa Involved in Nosocomial Infection as Revealed by RAPD-PCR Markers |
| title_fullStr | Genetic Fingerprinting of Pseudomonas aeruginosa Involved in Nosocomial Infection as Revealed by RAPD-PCR Markers |
| title_full_unstemmed | Genetic Fingerprinting of Pseudomonas aeruginosa Involved in Nosocomial Infection as Revealed by RAPD-PCR Markers |
| title_short | Genetic Fingerprinting of Pseudomonas aeruginosa Involved in Nosocomial Infection as Revealed by RAPD-PCR Markers |
| title_sort | genetic fingerprinting of pseudomonas aeruginosa involved in nosocomial infection as revealed by rapd pcr markers |
| topic | pseudomonas aeruginosa polymorphism genotype virulence antibiotic resistance |
| url | https://hdl.handle.net/10568/120190 |
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