The transcriptional cell atlas of testis development in sheep at pre-sexual maturity
Sheep testes undergo a dramatic rate of development with structural changes during pre-sexual maturity, including the proliferation and maturation of somatic niche cells and the initiation of spermatogenesis. To explore this complex process, 12,843 testicular cells from three males at pre-sexual mat...
| Autores principales: | , , , , , , , , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
MDPI
2022
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/119934 |
| _version_ | 1855540613671288832 |
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| author | Wu, Y. Guo, T. Li, J. Niu, C. Sun, W. Zhu, S. Zhao, H. Qiao, G. Han, M. He, X. Lu, Z. Yuan, C. Han Jianlin Liu, J. Yang, B. Yue, Y. |
| author_browse | Guo, T. Han Jianlin Han, M. He, X. Li, J. Liu, J. Lu, Z. Niu, C. Qiao, G. Sun, W. Wu, Y. Yang, B. Yuan, C. Yue, Y. Zhao, H. Zhu, S. |
| author_facet | Wu, Y. Guo, T. Li, J. Niu, C. Sun, W. Zhu, S. Zhao, H. Qiao, G. Han, M. He, X. Lu, Z. Yuan, C. Han Jianlin Liu, J. Yang, B. Yue, Y. |
| author_sort | Wu, Y. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Sheep testes undergo a dramatic rate of development with structural changes during pre-sexual maturity, including the proliferation and maturation of somatic niche cells and the initiation of spermatogenesis. To explore this complex process, 12,843 testicular cells from three males at pre-sexual maturity (three-month-old) were sequenced using the 10× Genomics ChromiumTM single-cell RNA-seq (scRNA-seq) technology. Nine testicular somatic cell types (Sertoli cells, myoid cells, monocytes, macrophages, Leydig cells, dendritic cells, endothelial cells, smooth muscle cells, and leukocytes) and an unknown cell cluster were observed. In particular, five male germ cell types (including two types of undifferentiated spermatogonia (Apale and Adark), primary spermatocytes, secondary spermatocytes, and sperm cells) were identified. Interestingly, Apale and Adark were found to be two distinct states of undifferentiated spermatogonia. Further analysis identified specific marker genes, including UCHL1, DDX4, SOHLH1, KITLG, and PCNA, in the germ cells at different states of differentiation. The study revealed significant changes in germline stem cells at pre-sexual maturation, paving the way to explore the candidate factors and pathways for the regulation of germ and somatic cells, and to provide us with opportunities for the establishment of livestock stem cell breeding programs. |
| format | Journal Article |
| id | CGSpace119934 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | CGSpace1199342025-12-08T10:29:22Z The transcriptional cell atlas of testis development in sheep at pre-sexual maturity Wu, Y. Guo, T. Li, J. Niu, C. Sun, W. Zhu, S. Zhao, H. Qiao, G. Han, M. He, X. Lu, Z. Yuan, C. Han Jianlin Liu, J. Yang, B. Yue, Y. sheep animal breeding small ruminants livestock Sheep testes undergo a dramatic rate of development with structural changes during pre-sexual maturity, including the proliferation and maturation of somatic niche cells and the initiation of spermatogenesis. To explore this complex process, 12,843 testicular cells from three males at pre-sexual maturity (three-month-old) were sequenced using the 10× Genomics ChromiumTM single-cell RNA-seq (scRNA-seq) technology. Nine testicular somatic cell types (Sertoli cells, myoid cells, monocytes, macrophages, Leydig cells, dendritic cells, endothelial cells, smooth muscle cells, and leukocytes) and an unknown cell cluster were observed. In particular, five male germ cell types (including two types of undifferentiated spermatogonia (Apale and Adark), primary spermatocytes, secondary spermatocytes, and sperm cells) were identified. Interestingly, Apale and Adark were found to be two distinct states of undifferentiated spermatogonia. Further analysis identified specific marker genes, including UCHL1, DDX4, SOHLH1, KITLG, and PCNA, in the germ cells at different states of differentiation. The study revealed significant changes in germline stem cells at pre-sexual maturation, paving the way to explore the candidate factors and pathways for the regulation of germ and somatic cells, and to provide us with opportunities for the establishment of livestock stem cell breeding programs. 2022-02-15 2022-06-24T13:14:08Z 2022-06-24T13:14:08Z Journal Article https://hdl.handle.net/10568/119934 en Open Access MDPI Wu, Y., Guo, T., Li, J., Niu, C., Sun, W., Zhu, S., Zhao, H., Qiao, G., Han, M., He, X., Lu, Z., Yuan, C., Han, J., Liu, J., Yang, B. and Yue, Y. 2022. The transcriptional cell atlas of testis development in sheep at pre-sexual maturity. Current issues in Molecular Biology 44(2):483-497. |
| spellingShingle | sheep animal breeding small ruminants livestock Wu, Y. Guo, T. Li, J. Niu, C. Sun, W. Zhu, S. Zhao, H. Qiao, G. Han, M. He, X. Lu, Z. Yuan, C. Han Jianlin Liu, J. Yang, B. Yue, Y. The transcriptional cell atlas of testis development in sheep at pre-sexual maturity |
| title | The transcriptional cell atlas of testis development in sheep at pre-sexual maturity |
| title_full | The transcriptional cell atlas of testis development in sheep at pre-sexual maturity |
| title_fullStr | The transcriptional cell atlas of testis development in sheep at pre-sexual maturity |
| title_full_unstemmed | The transcriptional cell atlas of testis development in sheep at pre-sexual maturity |
| title_short | The transcriptional cell atlas of testis development in sheep at pre-sexual maturity |
| title_sort | transcriptional cell atlas of testis development in sheep at pre sexual maturity |
| topic | sheep animal breeding small ruminants livestock |
| url | https://hdl.handle.net/10568/119934 |
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