An inexpensive CRISPR-based point-of-care test for the identification of meat species and meat products
The growing demand for and supply of meat and meat products has led to a proportional increase in cases of meat adulteration. Adulterated meat poses serious economic and health consequences globally. Current laboratory methods for meat species identification require specialized equipment with limite...
| Main Authors: | , , , , , , , , , , , , , , , |
|---|---|
| Format: | Journal Article |
| Language: | Inglés |
| Published: |
MDPI
2022
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/119686 |
| _version_ | 1855541296244981760 |
|---|---|
| author | Tao, D. Xiao, X. Lan, X. Xu, B. Wang, Y. Khazalwa, Emmanuel M. Pan, W. Ruan, J. Jiang, Y. Liu, X. Li, C. Ye, R. Li, X. Xu, J. Zhao, S. Xie, S. |
| author_browse | Jiang, Y. Khazalwa, Emmanuel M. Lan, X. Li, C. Li, X. Liu, X. Pan, W. Ruan, J. Tao, D. Wang, Y. Xiao, X. Xie, S. Xu, B. Xu, J. Ye, R. Zhao, S. |
| author_facet | Tao, D. Xiao, X. Lan, X. Xu, B. Wang, Y. Khazalwa, Emmanuel M. Pan, W. Ruan, J. Jiang, Y. Liu, X. Li, C. Ye, R. Li, X. Xu, J. Zhao, S. Xie, S. |
| author_sort | Tao, D. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The growing demand for and supply of meat and meat products has led to a proportional increase in cases of meat adulteration. Adulterated meat poses serious economic and health consequences globally. Current laboratory methods for meat species identification require specialized equipment with limited field applications. This study developed an inexpensive, point-of-care Loop-Mediated Isothermal Amplification (LAMP)-CRISPR/Cas12a colorimetric assay to detect meat species using a Texas Red-labelled single-strand (ssDNA) reporter. As low as 1.0 pg/µL of the porcine NADH4, the chicken NADH dehydrogenase subunit 2 (ND2) and the duck D-loop genes was detectable under white, blue and ultraviolet light. The test turnaround time from DNA extraction to visualization was approximately 40 min. The assay accurately detected pure and mixed-meat products in the laboratory (n = 15) and during a pilot point-of-care test (n = 8) in a food processing factory. The results are 100% reproducible using lateral flow detection strips and the real-time PCR detection instrument. This technology is fully deployable and usable in any standard room. Thus, our study demonstrates that this method is a straightforward, specific, sensitive, point-of-care test (POCT) adaptable to various outlets such as customs, quarantine units and meat import/export departments. |
| format | Journal Article |
| id | CGSpace119686 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | CGSpace1196862025-12-08T10:29:22Z An inexpensive CRISPR-based point-of-care test for the identification of meat species and meat products Tao, D. Xiao, X. Lan, X. Xu, B. Wang, Y. Khazalwa, Emmanuel M. Pan, W. Ruan, J. Jiang, Y. Liu, X. Li, C. Ye, R. Li, X. Xu, J. Zhao, S. Xie, S. animal products meat crispr The growing demand for and supply of meat and meat products has led to a proportional increase in cases of meat adulteration. Adulterated meat poses serious economic and health consequences globally. Current laboratory methods for meat species identification require specialized equipment with limited field applications. This study developed an inexpensive, point-of-care Loop-Mediated Isothermal Amplification (LAMP)-CRISPR/Cas12a colorimetric assay to detect meat species using a Texas Red-labelled single-strand (ssDNA) reporter. As low as 1.0 pg/µL of the porcine NADH4, the chicken NADH dehydrogenase subunit 2 (ND2) and the duck D-loop genes was detectable under white, blue and ultraviolet light. The test turnaround time from DNA extraction to visualization was approximately 40 min. The assay accurately detected pure and mixed-meat products in the laboratory (n = 15) and during a pilot point-of-care test (n = 8) in a food processing factory. The results are 100% reproducible using lateral flow detection strips and the real-time PCR detection instrument. This technology is fully deployable and usable in any standard room. Thus, our study demonstrates that this method is a straightforward, specific, sensitive, point-of-care test (POCT) adaptable to various outlets such as customs, quarantine units and meat import/export departments. 2022-05-19 2022-05-30T11:35:59Z 2022-05-30T11:35:59Z Journal Article https://hdl.handle.net/10568/119686 en Open Access MDPI Tao, D., Xiao, X., Lan, X., Xu, B., Wang, Y., Khazalwa, E.M., Pan, W., Ruan, J., Jiang, Y., Liu, X., Li, C., Ye, R., Li, X., Xu, J., Zhao, S. and Xie, S. 2022. An inexpensive CRISPR-based point-of-care test for the identification of meat species and meat products. Genes 13(5): 912. |
| spellingShingle | animal products meat crispr Tao, D. Xiao, X. Lan, X. Xu, B. Wang, Y. Khazalwa, Emmanuel M. Pan, W. Ruan, J. Jiang, Y. Liu, X. Li, C. Ye, R. Li, X. Xu, J. Zhao, S. Xie, S. An inexpensive CRISPR-based point-of-care test for the identification of meat species and meat products |
| title | An inexpensive CRISPR-based point-of-care test for the identification of meat species and meat products |
| title_full | An inexpensive CRISPR-based point-of-care test for the identification of meat species and meat products |
| title_fullStr | An inexpensive CRISPR-based point-of-care test for the identification of meat species and meat products |
| title_full_unstemmed | An inexpensive CRISPR-based point-of-care test for the identification of meat species and meat products |
| title_short | An inexpensive CRISPR-based point-of-care test for the identification of meat species and meat products |
| title_sort | inexpensive crispr based point of care test for the identification of meat species and meat products |
| topic | animal products meat crispr |
| url | https://hdl.handle.net/10568/119686 |
| work_keys_str_mv | AT taod aninexpensivecrisprbasedpointofcaretestfortheidentificationofmeatspeciesandmeatproducts AT xiaox aninexpensivecrisprbasedpointofcaretestfortheidentificationofmeatspeciesandmeatproducts AT lanx aninexpensivecrisprbasedpointofcaretestfortheidentificationofmeatspeciesandmeatproducts AT xub aninexpensivecrisprbasedpointofcaretestfortheidentificationofmeatspeciesandmeatproducts AT wangy aninexpensivecrisprbasedpointofcaretestfortheidentificationofmeatspeciesandmeatproducts AT khazalwaemmanuelm aninexpensivecrisprbasedpointofcaretestfortheidentificationofmeatspeciesandmeatproducts AT panw aninexpensivecrisprbasedpointofcaretestfortheidentificationofmeatspeciesandmeatproducts AT ruanj aninexpensivecrisprbasedpointofcaretestfortheidentificationofmeatspeciesandmeatproducts AT jiangy aninexpensivecrisprbasedpointofcaretestfortheidentificationofmeatspeciesandmeatproducts AT liux aninexpensivecrisprbasedpointofcaretestfortheidentificationofmeatspeciesandmeatproducts AT lic aninexpensivecrisprbasedpointofcaretestfortheidentificationofmeatspeciesandmeatproducts AT yer aninexpensivecrisprbasedpointofcaretestfortheidentificationofmeatspeciesandmeatproducts AT lix aninexpensivecrisprbasedpointofcaretestfortheidentificationofmeatspeciesandmeatproducts AT xuj aninexpensivecrisprbasedpointofcaretestfortheidentificationofmeatspeciesandmeatproducts AT zhaos aninexpensivecrisprbasedpointofcaretestfortheidentificationofmeatspeciesandmeatproducts AT xies aninexpensivecrisprbasedpointofcaretestfortheidentificationofmeatspeciesandmeatproducts AT taod inexpensivecrisprbasedpointofcaretestfortheidentificationofmeatspeciesandmeatproducts AT xiaox inexpensivecrisprbasedpointofcaretestfortheidentificationofmeatspeciesandmeatproducts AT lanx inexpensivecrisprbasedpointofcaretestfortheidentificationofmeatspeciesandmeatproducts AT xub inexpensivecrisprbasedpointofcaretestfortheidentificationofmeatspeciesandmeatproducts AT wangy inexpensivecrisprbasedpointofcaretestfortheidentificationofmeatspeciesandmeatproducts AT khazalwaemmanuelm inexpensivecrisprbasedpointofcaretestfortheidentificationofmeatspeciesandmeatproducts AT panw inexpensivecrisprbasedpointofcaretestfortheidentificationofmeatspeciesandmeatproducts AT ruanj inexpensivecrisprbasedpointofcaretestfortheidentificationofmeatspeciesandmeatproducts AT jiangy inexpensivecrisprbasedpointofcaretestfortheidentificationofmeatspeciesandmeatproducts AT liux inexpensivecrisprbasedpointofcaretestfortheidentificationofmeatspeciesandmeatproducts AT lic inexpensivecrisprbasedpointofcaretestfortheidentificationofmeatspeciesandmeatproducts AT yer inexpensivecrisprbasedpointofcaretestfortheidentificationofmeatspeciesandmeatproducts AT lix inexpensivecrisprbasedpointofcaretestfortheidentificationofmeatspeciesandmeatproducts AT xuj inexpensivecrisprbasedpointofcaretestfortheidentificationofmeatspeciesandmeatproducts AT zhaos inexpensivecrisprbasedpointofcaretestfortheidentificationofmeatspeciesandmeatproducts AT xies inexpensivecrisprbasedpointofcaretestfortheidentificationofmeatspeciesandmeatproducts |