From fruit growth to ripening in plantain: a careful balance between carbohydrate synthesis and breakdown
We investigated the fruit development in two plantain banana cultivars from two weeks after bunch emergence till twelve weeks through high-throughput proteomics, major metabolite quantification and metabolic flux analyses. We aimed to investigate for the first time different fruit development stages...
| Main Authors: | , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Oxford University Press
2022
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/119584 |
| _version_ | 1855534582012575744 |
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| author | Campos, Nadia A. Colombié, Sophie Moing, Annick Cassan, Cedric Amah, Delphine Swennen, Rony L. Gibon, Yves Carpentier, Sebastien C. |
| author_browse | Amah, Delphine Campos, Nadia A. Carpentier, Sebastien C. Cassan, Cedric Colombié, Sophie Gibon, Yves Moing, Annick Swennen, Rony L. |
| author_facet | Campos, Nadia A. Colombié, Sophie Moing, Annick Cassan, Cedric Amah, Delphine Swennen, Rony L. Gibon, Yves Carpentier, Sebastien C. |
| author_sort | Campos, Nadia A. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | We investigated the fruit development in two plantain banana cultivars from two weeks after bunch emergence till twelve weeks through high-throughput proteomics, major metabolite quantification and metabolic flux analyses. We aimed to investigate for the first time different fruit development stages and gain unique insights into the order of appearance and dominance of specific enzymes/fluxes. Starch synthesis and breakdown are processes that take place simultaneously. During the first ten weeks fruits accumulated up to 48% of starch. Glucose 6-phosphate and fructose were important starch precursors. We found a unique amyloplast transporter and hypothesize that it facilitates the import of fructose. We identified an invertase originating from the M. balbisiana genome that would enable to flow carbon back to growth and starch synthesis and keep a high starch content even during ripening. Enzymes associated to the initiation of ripening were involved in ethylene and auxin metabolism, starch breakdown, pulp softening and ascorbate biosynthesis. The initiation of ripening was cultivar specific. A faster initiation was particularly linked to 1-aminocyclopropane-1-carboxylate oxidase and 4-alpha glucanotransferase disproportioning enzyme. This knowledge is fundamental to determine the ideal harvest moment, reduce postharvest losses and improve product quality through breeding. |
| format | Journal Article |
| id | CGSpace119584 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Oxford University Press |
| publisherStr | Oxford University Press |
| record_format | dspace |
| spelling | CGSpace1195842025-11-11T19:01:39Z From fruit growth to ripening in plantain: a careful balance between carbohydrate synthesis and breakdown Campos, Nadia A. Colombié, Sophie Moing, Annick Cassan, Cedric Amah, Delphine Swennen, Rony L. Gibon, Yves Carpentier, Sebastien C. ripening metabolites starch crops proteomics maduramiento metabolitos plantas feculentas We investigated the fruit development in two plantain banana cultivars from two weeks after bunch emergence till twelve weeks through high-throughput proteomics, major metabolite quantification and metabolic flux analyses. We aimed to investigate for the first time different fruit development stages and gain unique insights into the order of appearance and dominance of specific enzymes/fluxes. Starch synthesis and breakdown are processes that take place simultaneously. During the first ten weeks fruits accumulated up to 48% of starch. Glucose 6-phosphate and fructose were important starch precursors. We found a unique amyloplast transporter and hypothesize that it facilitates the import of fructose. We identified an invertase originating from the M. balbisiana genome that would enable to flow carbon back to growth and starch synthesis and keep a high starch content even during ripening. Enzymes associated to the initiation of ripening were involved in ethylene and auxin metabolism, starch breakdown, pulp softening and ascorbate biosynthesis. The initiation of ripening was cultivar specific. A faster initiation was particularly linked to 1-aminocyclopropane-1-carboxylate oxidase and 4-alpha glucanotransferase disproportioning enzyme. This knowledge is fundamental to determine the ideal harvest moment, reduce postharvest losses and improve product quality through breeding. 2022-08-11 2022-05-18T10:27:24Z 2022-05-18T10:27:24Z Journal Article https://hdl.handle.net/10568/119584 en Open Access application/pdf Oxford University Press Campos, N.A.; Colombié, S.; Moing, A.; Cassan, C.; Amah, D.; Swennen, R.; Gibon, Y.; Carpentier, S.C. (2022) From fruit growth to ripening in plantain: a careful balance between carbohydrate synthesis and breakdown. Journal of Experimental Botany 73(14) p. 4832–4849 18 p. ISSN: 0022-0957 |
| spellingShingle | ripening metabolites starch crops proteomics maduramiento metabolitos plantas feculentas Campos, Nadia A. Colombié, Sophie Moing, Annick Cassan, Cedric Amah, Delphine Swennen, Rony L. Gibon, Yves Carpentier, Sebastien C. From fruit growth to ripening in plantain: a careful balance between carbohydrate synthesis and breakdown |
| title | From fruit growth to ripening in plantain: a careful balance between carbohydrate synthesis and breakdown |
| title_full | From fruit growth to ripening in plantain: a careful balance between carbohydrate synthesis and breakdown |
| title_fullStr | From fruit growth to ripening in plantain: a careful balance between carbohydrate synthesis and breakdown |
| title_full_unstemmed | From fruit growth to ripening in plantain: a careful balance between carbohydrate synthesis and breakdown |
| title_short | From fruit growth to ripening in plantain: a careful balance between carbohydrate synthesis and breakdown |
| title_sort | from fruit growth to ripening in plantain a careful balance between carbohydrate synthesis and breakdown |
| topic | ripening metabolites starch crops proteomics maduramiento metabolitos plantas feculentas |
| url | https://hdl.handle.net/10568/119584 |
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