Detection of antimicrobial resistance, pathogenicity, and virulence potentials of non-typhoidal Salmonella isolates at the Yaounde abattoir using whole-genome sequencing technique
One of the crucial public health problems today is the emerging and re-emerging of multidrug-resistant (MDR) bacteria coupled with a decline in the development of new antimicrobials. Non-typhoidal Salmonella (NTS) is classified among the MDR pathogens of international concern. To predict their MDR p...
| Autores principales: | , , , , , , , , , , , , , |
|---|---|
| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
MDPI
2022
|
| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/119461 |
| _version_ | 1855524526804172800 |
|---|---|
| author | Matchawe, C. Machuka, Eunice M. Kyallo, Martina M. Bonny, P. Nkeunen, G. Njaci, Isaac Esemu, S.N. Githae, Dedan Juma, John Nfor, B.M. Nsawir, B.J. Piasentier, E. Ndip, L.M. Pelle, Roger |
| author_browse | Bonny, P. Esemu, S.N. Githae, Dedan Juma, John Kyallo, Martina M. Machuka, Eunice M. Matchawe, C. Ndip, L.M. Nfor, B.M. Njaci, Isaac Nkeunen, G. Nsawir, B.J. Pelle, Roger Piasentier, E. |
| author_facet | Matchawe, C. Machuka, Eunice M. Kyallo, Martina M. Bonny, P. Nkeunen, G. Njaci, Isaac Esemu, S.N. Githae, Dedan Juma, John Nfor, B.M. Nsawir, B.J. Piasentier, E. Ndip, L.M. Pelle, Roger |
| author_sort | Matchawe, C. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | One of the crucial public health problems today is the emerging and re-emerging of multidrug-resistant (MDR) bacteria coupled with a decline in the development of new antimicrobials. Non-typhoidal Salmonella (NTS) is classified among the MDR pathogens of international concern. To predict their MDR potentials, 23 assembled genomes of NTS from live cattle (n = 1), beef carcass (n = 19), butchers’ hands (n = 1) and beef processing environments (n = 2) isolated from 830 wet swabs at the Yaounde abattoir between December 2014 and November 2015 were explored using whole-genome sequencing. Phenotypically, while 22% (n = 5) of Salmonella isolates were streptomycin-resistant, 13% (n = 3) were MDR. Genotypically, all the Salmonella isolates possessed high MDR potentials against several classes of antibiotics including critically important drugs (carbapenems, third-generation cephalosporin and fluoroquinolone). Moreover, >31% of NTS exhibited resistance potentials to polymyxin, considered as the last resort drug. Additionally, ≤80% of isolates harbored “silent resistant genes” as a potential reservoir of drug resistance. Our isolates showed a high degree of pathogenicity and possessed key virulence factors to establish infection even in humans. Whole-genome sequencing unveiled both broader antimicrobial resistance (AMR) profiles and inference of pathogen characteristics. This study calls for the prudent use of antibiotics and constant monitoring of AMR of NTS. |
| format | Journal Article |
| id | CGSpace119461 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | CGSpace1194612025-12-08T10:29:22Z Detection of antimicrobial resistance, pathogenicity, and virulence potentials of non-typhoidal Salmonella isolates at the Yaounde abattoir using whole-genome sequencing technique Matchawe, C. Machuka, Eunice M. Kyallo, Martina M. Bonny, P. Nkeunen, G. Njaci, Isaac Esemu, S.N. Githae, Dedan Juma, John Nfor, B.M. Nsawir, B.J. Piasentier, E. Ndip, L.M. Pelle, Roger animal diseases antimicrobial resistance genetics One of the crucial public health problems today is the emerging and re-emerging of multidrug-resistant (MDR) bacteria coupled with a decline in the development of new antimicrobials. Non-typhoidal Salmonella (NTS) is classified among the MDR pathogens of international concern. To predict their MDR potentials, 23 assembled genomes of NTS from live cattle (n = 1), beef carcass (n = 19), butchers’ hands (n = 1) and beef processing environments (n = 2) isolated from 830 wet swabs at the Yaounde abattoir between December 2014 and November 2015 were explored using whole-genome sequencing. Phenotypically, while 22% (n = 5) of Salmonella isolates were streptomycin-resistant, 13% (n = 3) were MDR. Genotypically, all the Salmonella isolates possessed high MDR potentials against several classes of antibiotics including critically important drugs (carbapenems, third-generation cephalosporin and fluoroquinolone). Moreover, >31% of NTS exhibited resistance potentials to polymyxin, considered as the last resort drug. Additionally, ≤80% of isolates harbored “silent resistant genes” as a potential reservoir of drug resistance. Our isolates showed a high degree of pathogenicity and possessed key virulence factors to establish infection even in humans. Whole-genome sequencing unveiled both broader antimicrobial resistance (AMR) profiles and inference of pathogen characteristics. This study calls for the prudent use of antibiotics and constant monitoring of AMR of NTS. 2022-04-23 2022-05-04T17:35:41Z 2022-05-04T17:35:41Z Journal Article https://hdl.handle.net/10568/119461 en Open Access application/pdf MDPI Matchawe, C., Machuka, E.M., Kyallo, M., Bonny, P., Nkeunen, G., Njaci, I., Esemu, S.N., Githae, D., Juma, J., Nfor, B.M., Nsawir, B.J., Piasentier, E., Ndip, L.M. and Pelle, R. 2022. Detection of antimicrobial resistance, pathogenicity, and virulence potentials of non-typhoidal Salmonella isolates at the Yaounde abattoir using whole-genome sequencing technique. Pathogens 11:502. |
| spellingShingle | animal diseases antimicrobial resistance genetics Matchawe, C. Machuka, Eunice M. Kyallo, Martina M. Bonny, P. Nkeunen, G. Njaci, Isaac Esemu, S.N. Githae, Dedan Juma, John Nfor, B.M. Nsawir, B.J. Piasentier, E. Ndip, L.M. Pelle, Roger Detection of antimicrobial resistance, pathogenicity, and virulence potentials of non-typhoidal Salmonella isolates at the Yaounde abattoir using whole-genome sequencing technique |
| title | Detection of antimicrobial resistance, pathogenicity, and virulence potentials of non-typhoidal Salmonella isolates at the Yaounde abattoir using whole-genome sequencing technique |
| title_full | Detection of antimicrobial resistance, pathogenicity, and virulence potentials of non-typhoidal Salmonella isolates at the Yaounde abattoir using whole-genome sequencing technique |
| title_fullStr | Detection of antimicrobial resistance, pathogenicity, and virulence potentials of non-typhoidal Salmonella isolates at the Yaounde abattoir using whole-genome sequencing technique |
| title_full_unstemmed | Detection of antimicrobial resistance, pathogenicity, and virulence potentials of non-typhoidal Salmonella isolates at the Yaounde abattoir using whole-genome sequencing technique |
| title_short | Detection of antimicrobial resistance, pathogenicity, and virulence potentials of non-typhoidal Salmonella isolates at the Yaounde abattoir using whole-genome sequencing technique |
| title_sort | detection of antimicrobial resistance pathogenicity and virulence potentials of non typhoidal salmonella isolates at the yaounde abattoir using whole genome sequencing technique |
| topic | animal diseases antimicrobial resistance genetics |
| url | https://hdl.handle.net/10568/119461 |
| work_keys_str_mv | AT matchawec detectionofantimicrobialresistancepathogenicityandvirulencepotentialsofnontyphoidalsalmonellaisolatesattheyaoundeabattoirusingwholegenomesequencingtechnique AT machukaeunicem detectionofantimicrobialresistancepathogenicityandvirulencepotentialsofnontyphoidalsalmonellaisolatesattheyaoundeabattoirusingwholegenomesequencingtechnique AT kyallomartinam detectionofantimicrobialresistancepathogenicityandvirulencepotentialsofnontyphoidalsalmonellaisolatesattheyaoundeabattoirusingwholegenomesequencingtechnique AT bonnyp detectionofantimicrobialresistancepathogenicityandvirulencepotentialsofnontyphoidalsalmonellaisolatesattheyaoundeabattoirusingwholegenomesequencingtechnique AT nkeuneng detectionofantimicrobialresistancepathogenicityandvirulencepotentialsofnontyphoidalsalmonellaisolatesattheyaoundeabattoirusingwholegenomesequencingtechnique AT njaciisaac detectionofantimicrobialresistancepathogenicityandvirulencepotentialsofnontyphoidalsalmonellaisolatesattheyaoundeabattoirusingwholegenomesequencingtechnique AT esemusn detectionofantimicrobialresistancepathogenicityandvirulencepotentialsofnontyphoidalsalmonellaisolatesattheyaoundeabattoirusingwholegenomesequencingtechnique AT githaededan detectionofantimicrobialresistancepathogenicityandvirulencepotentialsofnontyphoidalsalmonellaisolatesattheyaoundeabattoirusingwholegenomesequencingtechnique AT jumajohn detectionofantimicrobialresistancepathogenicityandvirulencepotentialsofnontyphoidalsalmonellaisolatesattheyaoundeabattoirusingwholegenomesequencingtechnique AT nforbm detectionofantimicrobialresistancepathogenicityandvirulencepotentialsofnontyphoidalsalmonellaisolatesattheyaoundeabattoirusingwholegenomesequencingtechnique AT nsawirbj detectionofantimicrobialresistancepathogenicityandvirulencepotentialsofnontyphoidalsalmonellaisolatesattheyaoundeabattoirusingwholegenomesequencingtechnique AT piasentiere detectionofantimicrobialresistancepathogenicityandvirulencepotentialsofnontyphoidalsalmonellaisolatesattheyaoundeabattoirusingwholegenomesequencingtechnique AT ndiplm detectionofantimicrobialresistancepathogenicityandvirulencepotentialsofnontyphoidalsalmonellaisolatesattheyaoundeabattoirusingwholegenomesequencingtechnique AT pelleroger detectionofantimicrobialresistancepathogenicityandvirulencepotentialsofnontyphoidalsalmonellaisolatesattheyaoundeabattoirusingwholegenomesequencingtechnique |