Paternity assignment in white guinea yam (Dioscorea Rotundata) half-sib Progenies from polycross mating design using SNP markers
White Guinea yam is mostly a dioecious outcrossing crop with male and female flowers produced on distinct plants. Fertile parents produce high fruit set in an open pollination polycross block, which is a cost-effective and convenient way of generating variability in yam breeding. However, the pollen...
| Main Authors: | , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
MDPI
2020
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/119459 |
| _version_ | 1855514258239913984 |
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| author | Norman, P.E. Agre, P.A. Danquah, A. Tongoona, P.B. Danquah, E.Y. Koeyer, D. de Ikeogu, Ugochukwu N. Asiedu, R. Asfaw, A. |
| author_browse | Agre, P.A. Asfaw, A. Asiedu, R. Danquah, A. Danquah, E.Y. Ikeogu, Ugochukwu N. Koeyer, D. de Norman, P.E. Tongoona, P.B. |
| author_facet | Norman, P.E. Agre, P.A. Danquah, A. Tongoona, P.B. Danquah, E.Y. Koeyer, D. de Ikeogu, Ugochukwu N. Asiedu, R. Asfaw, A. |
| author_sort | Norman, P.E. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | White Guinea yam is mostly a dioecious outcrossing crop with male and female flowers produced on distinct plants. Fertile parents produce high fruit set in an open pollination polycross block, which is a cost-effective and convenient way of generating variability in yam breeding. However, the pollen parent of progeny from polycross mating is usually unknown. This study aimed to determine paternity in white Guinea yam half-sib progenies from polycross mating design. A total of 394 half-sib progenies from random open pollination involving nine female and three male parents was genotyped with 6602 SNP markers from DArTSeq platform to recover full pedigree. A higher proportion of expected heterozygosity, allelic richness, and evenness were observed in the half-sib progenies. A complete pedigree was established for all progenies from two families (TDr1685 and TDr1688) with 100% accuracy, while in the remaining families, paternity was assigned successfully only for 56 to 98% of the progenies. Our results indicated unequal paternal contribution under natural open pollination in yam, suggesting unequal pollen migrations or gene flow among the crossing parents. A total of 3.8% of progenies lacking paternal identity due to foreign pollen contamination outside the polycross block was observed. This study established the efficient determination of parental reconstruction and allelic contributions in the white Guinea yam half-sib progenies generated from open pollination polycross using SNP markers. Findings are useful for parental reconstruction, accurate dissection of the genetic effects, and selection in white Guinea yam breeding program utilizing polycross mating design. |
| format | Journal Article |
| id | CGSpace119459 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | CGSpace1194592025-11-11T10:01:55Z Paternity assignment in white guinea yam (Dioscorea Rotundata) half-sib Progenies from polycross mating design using SNP markers Norman, P.E. Agre, P.A. Danquah, A. Tongoona, P.B. Danquah, E.Y. Koeyer, D. de Ikeogu, Ugochukwu N. Asiedu, R. Asfaw, A. yams dioscorea rotundata dna food production paternity ecology White Guinea yam is mostly a dioecious outcrossing crop with male and female flowers produced on distinct plants. Fertile parents produce high fruit set in an open pollination polycross block, which is a cost-effective and convenient way of generating variability in yam breeding. However, the pollen parent of progeny from polycross mating is usually unknown. This study aimed to determine paternity in white Guinea yam half-sib progenies from polycross mating design. A total of 394 half-sib progenies from random open pollination involving nine female and three male parents was genotyped with 6602 SNP markers from DArTSeq platform to recover full pedigree. A higher proportion of expected heterozygosity, allelic richness, and evenness were observed in the half-sib progenies. A complete pedigree was established for all progenies from two families (TDr1685 and TDr1688) with 100% accuracy, while in the remaining families, paternity was assigned successfully only for 56 to 98% of the progenies. Our results indicated unequal paternal contribution under natural open pollination in yam, suggesting unequal pollen migrations or gene flow among the crossing parents. A total of 3.8% of progenies lacking paternal identity due to foreign pollen contamination outside the polycross block was observed. This study established the efficient determination of parental reconstruction and allelic contributions in the white Guinea yam half-sib progenies generated from open pollination polycross using SNP markers. Findings are useful for parental reconstruction, accurate dissection of the genetic effects, and selection in white Guinea yam breeding program utilizing polycross mating design. 2020 2022-05-04T11:53:45Z 2022-05-04T11:53:45Z Journal Article https://hdl.handle.net/10568/119459 en Open Access application/pdf MDPI Norman, P.E., Agre, P.A., Danquah, A., Tongoona, P.B., Danquah, E.Y., De Koeyer, D., ... & Asfaw, A. (2020). Paternity assignment in white guinea yam (Dioscorea Rotundata) half-sib Progenies from polycross mating design using SNP markers. Plants, 9(4): 527, 1-15. |
| spellingShingle | yams dioscorea rotundata dna food production paternity ecology Norman, P.E. Agre, P.A. Danquah, A. Tongoona, P.B. Danquah, E.Y. Koeyer, D. de Ikeogu, Ugochukwu N. Asiedu, R. Asfaw, A. Paternity assignment in white guinea yam (Dioscorea Rotundata) half-sib Progenies from polycross mating design using SNP markers |
| title | Paternity assignment in white guinea yam (Dioscorea Rotundata) half-sib Progenies from polycross mating design using SNP markers |
| title_full | Paternity assignment in white guinea yam (Dioscorea Rotundata) half-sib Progenies from polycross mating design using SNP markers |
| title_fullStr | Paternity assignment in white guinea yam (Dioscorea Rotundata) half-sib Progenies from polycross mating design using SNP markers |
| title_full_unstemmed | Paternity assignment in white guinea yam (Dioscorea Rotundata) half-sib Progenies from polycross mating design using SNP markers |
| title_short | Paternity assignment in white guinea yam (Dioscorea Rotundata) half-sib Progenies from polycross mating design using SNP markers |
| title_sort | paternity assignment in white guinea yam dioscorea rotundata half sib progenies from polycross mating design using snp markers |
| topic | yams dioscorea rotundata dna food production paternity ecology |
| url | https://hdl.handle.net/10568/119459 |
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