Micropropagation and effect of phloroglucinol on rooting of Diospyros crassiflora Hiern
The jet-black to streaked ebony wood produced by the African ebony (Diospyros crassiflora Hiern, Ebenaceae) is exploited in Central and West Africa. A conservation effort is currently underway in Cameroon to propagate the tree through seedlings and cuttings. However, the intermittent availability of...
| Autores principales: | , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
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American Society for Horticultural Science
2020
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| Acceso en línea: | https://hdl.handle.net/10568/119377 |
| _version_ | 1855524229562236928 |
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| author | Tchouga, A.O. Deblauwe, V. Djabou, S.A.M. Forgione, G. Hanna, R. Niemenak, N. |
| author_browse | Deblauwe, V. Djabou, S.A.M. Forgione, G. Hanna, R. Niemenak, N. Tchouga, A.O. |
| author_facet | Tchouga, A.O. Deblauwe, V. Djabou, S.A.M. Forgione, G. Hanna, R. Niemenak, N. |
| author_sort | Tchouga, A.O. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The jet-black to streaked ebony wood produced by the African ebony (Diospyros crassiflora Hiern, Ebenaceae) is exploited in Central and West Africa. A conservation effort is currently underway in Cameroon to propagate the tree through seedlings and cuttings. However, the intermittent availability of its seeds and the long time required for rooting formation of cuttings are limiting its propagation. This study aims to develop a successful protocol for ebony micropropagation. In vitro culture of nodal segments from seedlings was performed in half-strength Murashige and Skoog (MS) medium supplemented with either zeatin (0.0, 2.3, 4.6, 9.1, 13.7, 18.2, 22.8, and 27.4 µm) or 6-benzylaminopurine (BAP) (0.0, 2.2, 4.4, 8.8, 13.3, 17.8, 22.2, and 26.6 µm). After 12 weeks, all media allowed shoot budbreak. Shoots displaying the greatest budbreak were observed with 22.8 µm zeatin and 22.2 µm BAP. The shoots were then transferred to a medium supplemented with indole-3-butyric acid (IBA) and phloroglucinol (PG) at different concentrations for root induction. Root induction was observed on the shoots initially induced in the medium with BAP, but not in those grown in the medium with zeatin. In half-strength MS supplemented with 396.5 µm PG plus 14.2 µm IBA, the formation of a single tap root was observed on 79% of shoots, with an average root length of 2.8 ± 0.18 cm. Rooted plants were successfully acclimatized to the greenhouse, with a 50% survival rate. This is the first report on Diospyros crassiflora micropropagation, which paves the way for rapid ebony multiplication to respond to needed conservation efforts. |
| format | Journal Article |
| id | CGSpace119377 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | American Society for Horticultural Science |
| publisherStr | American Society for Horticultural Science |
| record_format | dspace |
| spelling | CGSpace1193772025-11-11T10:15:58Z Micropropagation and effect of phloroglucinol on rooting of Diospyros crassiflora Hiern Tchouga, A.O. Deblauwe, V. Djabou, S.A.M. Forgione, G. Hanna, R. Niemenak, N. in vitro culture microcuttings plant growth substances micropropagation The jet-black to streaked ebony wood produced by the African ebony (Diospyros crassiflora Hiern, Ebenaceae) is exploited in Central and West Africa. A conservation effort is currently underway in Cameroon to propagate the tree through seedlings and cuttings. However, the intermittent availability of its seeds and the long time required for rooting formation of cuttings are limiting its propagation. This study aims to develop a successful protocol for ebony micropropagation. In vitro culture of nodal segments from seedlings was performed in half-strength Murashige and Skoog (MS) medium supplemented with either zeatin (0.0, 2.3, 4.6, 9.1, 13.7, 18.2, 22.8, and 27.4 µm) or 6-benzylaminopurine (BAP) (0.0, 2.2, 4.4, 8.8, 13.3, 17.8, 22.2, and 26.6 µm). After 12 weeks, all media allowed shoot budbreak. Shoots displaying the greatest budbreak were observed with 22.8 µm zeatin and 22.2 µm BAP. The shoots were then transferred to a medium supplemented with indole-3-butyric acid (IBA) and phloroglucinol (PG) at different concentrations for root induction. Root induction was observed on the shoots initially induced in the medium with BAP, but not in those grown in the medium with zeatin. In half-strength MS supplemented with 396.5 µm PG plus 14.2 µm IBA, the formation of a single tap root was observed on 79% of shoots, with an average root length of 2.8 ± 0.18 cm. Rooted plants were successfully acclimatized to the greenhouse, with a 50% survival rate. This is the first report on Diospyros crassiflora micropropagation, which paves the way for rapid ebony multiplication to respond to needed conservation efforts. 2020-04 2022-04-25T10:18:26Z 2022-04-25T10:18:26Z Journal Article https://hdl.handle.net/10568/119377 en Open Access application/pdf American Society for Horticultural Science Tchouga, A.O., Deblauwe, V., Djabou, S.A.M., Forgione, G., Hanna, R. & Niemenak, N. (2020). Micropropagation and effect of phloroglucinol on rooting of Diospyros crassiflora Hiern. HortScience, 55(4), 424-428. |
| spellingShingle | in vitro culture microcuttings plant growth substances micropropagation Tchouga, A.O. Deblauwe, V. Djabou, S.A.M. Forgione, G. Hanna, R. Niemenak, N. Micropropagation and effect of phloroglucinol on rooting of Diospyros crassiflora Hiern |
| title | Micropropagation and effect of phloroglucinol on rooting of Diospyros crassiflora Hiern |
| title_full | Micropropagation and effect of phloroglucinol on rooting of Diospyros crassiflora Hiern |
| title_fullStr | Micropropagation and effect of phloroglucinol on rooting of Diospyros crassiflora Hiern |
| title_full_unstemmed | Micropropagation and effect of phloroglucinol on rooting of Diospyros crassiflora Hiern |
| title_short | Micropropagation and effect of phloroglucinol on rooting of Diospyros crassiflora Hiern |
| title_sort | micropropagation and effect of phloroglucinol on rooting of diospyros crassiflora hiern |
| topic | in vitro culture microcuttings plant growth substances micropropagation |
| url | https://hdl.handle.net/10568/119377 |
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