Mediation of potato-potato cyst nematode, G. rostochiensis interaction by specific root exudate compounds
Potato (Solanum tuberosum) is a widely consumed staple food crop worldwide whose production is threatened by potato cyst nematodes (PCN). To infect a host, PCN eggs first need to be stimulated to hatch by chemical components in the host root exudates, yet it remains unknown how most root exudate com...
| Autores principales: | , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Frontiers Media
2020
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/119316 |
| _version_ | 1855526263715790848 |
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| author | Ochola, J. Cortada Gonzales, L. Ng'ang'a, M. Hassanali, A. Coyne, D. Torto, B. |
| author_browse | Cortada Gonzales, L. Coyne, D. Hassanali, A. Ng'ang'a, M. Ochola, J. Torto, B. |
| author_facet | Ochola, J. Cortada Gonzales, L. Ng'ang'a, M. Hassanali, A. Coyne, D. Torto, B. |
| author_sort | Ochola, J. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Potato (Solanum tuberosum) is a widely consumed staple food crop worldwide whose production is threatened by potato cyst nematodes (PCN). To infect a host, PCN eggs first need to be stimulated to hatch by chemical components in the host root exudates, yet it remains unknown how most root exudate components influence PCN behavior. Here, we evaluated the influence of eight compounds identified by LC-QqQ-MS in the root exudate of potato on the hatching response of the PCN, Globodera rostochiensis at varying doses. The eight compounds included the amino acids tyrosine, tryptophan and phenylalanine; phytohormones zeatin and methyl dihydrojasmonate; steroidal glycoalkaloids α-solanine and α-chaconine and the steroidal alkaloid solanidine. We additionally tested two other Solanaceae steroidal alkaloids, solasodine and tomatidine, previously identified in the root exudates of tomato, an alternative host for PCN. In dose-response assays with the individual compounds, the known PCN hatching factors α-chaconine and α-solanine stimulated the highest number of eggs to hatch, ∼47 and ∼42%, respectively, whereas the steroidal alkaloids (aglycones), solanidine and solasodine and potato root exudate (PRE) were intermediate, 28% each and 21%, respectively, with tomatidine eliciting the lowest hatching response 13%. However, ∼60% of the hatched juveniles failed to emerge from the cyst, which was compound- and concentration-dependent. The amino acids, phytohormones and the negative control (1% DMSO in water), however, were generally non-stimulatory. The use of steroidal glycoalkaloids and their aglycones in the suicidal hatching of PCN offers promise as an environmentally sustainable approach to manage this pest. |
| format | Journal Article |
| id | CGSpace119316 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Frontiers Media |
| publisherStr | Frontiers Media |
| record_format | dspace |
| spelling | CGSpace1193162025-11-11T10:18:35Z Mediation of potato-potato cyst nematode, G. rostochiensis interaction by specific root exudate compounds Ochola, J. Cortada Gonzales, L. Ng'ang'a, M. Hassanali, A. Coyne, D. Torto, B. potatoes globodera hatcheries semiochemicals steroids glycoalkaloids Potato (Solanum tuberosum) is a widely consumed staple food crop worldwide whose production is threatened by potato cyst nematodes (PCN). To infect a host, PCN eggs first need to be stimulated to hatch by chemical components in the host root exudates, yet it remains unknown how most root exudate components influence PCN behavior. Here, we evaluated the influence of eight compounds identified by LC-QqQ-MS in the root exudate of potato on the hatching response of the PCN, Globodera rostochiensis at varying doses. The eight compounds included the amino acids tyrosine, tryptophan and phenylalanine; phytohormones zeatin and methyl dihydrojasmonate; steroidal glycoalkaloids α-solanine and α-chaconine and the steroidal alkaloid solanidine. We additionally tested two other Solanaceae steroidal alkaloids, solasodine and tomatidine, previously identified in the root exudates of tomato, an alternative host for PCN. In dose-response assays with the individual compounds, the known PCN hatching factors α-chaconine and α-solanine stimulated the highest number of eggs to hatch, ∼47 and ∼42%, respectively, whereas the steroidal alkaloids (aglycones), solanidine and solasodine and potato root exudate (PRE) were intermediate, 28% each and 21%, respectively, with tomatidine eliciting the lowest hatching response 13%. However, ∼60% of the hatched juveniles failed to emerge from the cyst, which was compound- and concentration-dependent. The amino acids, phytohormones and the negative control (1% DMSO in water), however, were generally non-stimulatory. The use of steroidal glycoalkaloids and their aglycones in the suicidal hatching of PCN offers promise as an environmentally sustainable approach to manage this pest. 2020 2022-04-13T08:38:48Z 2022-04-13T08:38:48Z Journal Article https://hdl.handle.net/10568/119316 en Open Access application/pdf Frontiers Media Ochola, J., Cortada, L., Ng’ang’a, M., Hassanali, A., Coyne, D. & Torto, B. (2020). Mediation of potato-potato cyst nematode, G. rostochiensis interaction by specific root exudate compounds. Frontiers in Plant Science, 11: 649, 1-11. |
| spellingShingle | potatoes globodera hatcheries semiochemicals steroids glycoalkaloids Ochola, J. Cortada Gonzales, L. Ng'ang'a, M. Hassanali, A. Coyne, D. Torto, B. Mediation of potato-potato cyst nematode, G. rostochiensis interaction by specific root exudate compounds |
| title | Mediation of potato-potato cyst nematode, G. rostochiensis interaction by specific root exudate compounds |
| title_full | Mediation of potato-potato cyst nematode, G. rostochiensis interaction by specific root exudate compounds |
| title_fullStr | Mediation of potato-potato cyst nematode, G. rostochiensis interaction by specific root exudate compounds |
| title_full_unstemmed | Mediation of potato-potato cyst nematode, G. rostochiensis interaction by specific root exudate compounds |
| title_short | Mediation of potato-potato cyst nematode, G. rostochiensis interaction by specific root exudate compounds |
| title_sort | mediation of potato potato cyst nematode g rostochiensis interaction by specific root exudate compounds |
| topic | potatoes globodera hatcheries semiochemicals steroids glycoalkaloids |
| url | https://hdl.handle.net/10568/119316 |
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