Analysis of antimicrobial resistance in non-typhoidal Salmonella collected from pork retail outlets in Vietnam using whole genome sequencing
Non-typhoidal salmonella (TS) remains a significant health burden worldwide. In Vietnam, pork accounts for 70% of the total meat consumed, and contamination with Salmonella is high. High levels of antimicrobial resistance (AMR) have emerged among porcine NTS and of particular concern is the emergenc...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Frontiers Media
2022
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/119225 |
| _version_ | 1855536593479139328 |
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| author | Holohan, N. Wallat, M. Thi Hai Yen Luu Clark, E. Duong Thi Quy Truong Sinh Dang-Xuan Hue Thi Kim Vu Dung Van Truong Hoang Tran Huy Hung Nguyen-Viet Unger, Fred Son Thi Thanh Dang Stabler, R.A. |
| author_browse | Clark, E. Dung Van Truong Duong Thi Quy Truong Hoang Tran Huy Holohan, N. Hue Thi Kim Vu Hung Nguyen-Viet Sinh Dang-Xuan Son Thi Thanh Dang Stabler, R.A. Thi Hai Yen Luu Unger, Fred Wallat, M. |
| author_facet | Holohan, N. Wallat, M. Thi Hai Yen Luu Clark, E. Duong Thi Quy Truong Sinh Dang-Xuan Hue Thi Kim Vu Dung Van Truong Hoang Tran Huy Hung Nguyen-Viet Unger, Fred Son Thi Thanh Dang Stabler, R.A. |
| author_sort | Holohan, N. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Non-typhoidal salmonella (TS) remains a significant health burden worldwide. In Vietnam, pork accounts for 70% of the total meat consumed, and contamination with Salmonella is high. High levels of antimicrobial resistance (AMR) have emerged among porcine NTS and of particular concern is the emergence of colistin resistance, a “last defense” antibioic against multi-drug resistant (MDR) Gram-negative pathogens. This study aimed to investigate the antibiotic susceptibility of 69 NTS isolates collected from the pork retail outlets and slaughterhouses in Vietnam during 2014 a nd 2018/19. Phenotypic testing and whole genome sequencing was used to assess the serotype and AMR gene profiles of the 69 NTS isolates. Seventeen different serotypes were identified, of which S. enterica subsp enterica serotype Typhimurium was the most common followed by S. ser. Rissen, S. ser. London, S. ser. Anatum, and S. ser. Derby. Phenotype AMR was common with 41 (59.4%) isolates deemed MDR. MDR strains were most common in slaughterhouses (83%) and supermarkets (75%) and lowest in traditional markets (38%) and convenience stores (40%). Colistin resistance was identified in 18 strains (15 resistant, three intermediate) with mcr-1 identified in seven isolates (S. ser. Meleagridis, S. Rissen, S. Derby) and mcr-3 in two isolates (S. Typhimurium). This includes the first mcr positive S. Meleagridis to our knowledge. Surprisingly, boutique stores had high levels (60%) of MDR isolates including 5/20 isolates with mcr-1. This study demonstrates that pork from modern retail stores classed as supermarkets or boutique (with pork claiming to be high quality, traceable, environmentally friendly marketed toward higher income consumers) still contained NTS with high levels of AMR. |
| format | Journal Article |
| id | CGSpace119225 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Frontiers Media |
| publisherStr | Frontiers Media |
| record_format | dspace |
| spelling | CGSpace1192252025-12-08T10:29:22Z Analysis of antimicrobial resistance in non-typhoidal Salmonella collected from pork retail outlets in Vietnam using whole genome sequencing Holohan, N. Wallat, M. Thi Hai Yen Luu Clark, E. Duong Thi Quy Truong Sinh Dang-Xuan Hue Thi Kim Vu Dung Van Truong Hoang Tran Huy Hung Nguyen-Viet Unger, Fred Son Thi Thanh Dang Stabler, R.A. antimicrobial resistance animal products swine Non-typhoidal salmonella (TS) remains a significant health burden worldwide. In Vietnam, pork accounts for 70% of the total meat consumed, and contamination with Salmonella is high. High levels of antimicrobial resistance (AMR) have emerged among porcine NTS and of particular concern is the emergence of colistin resistance, a “last defense” antibioic against multi-drug resistant (MDR) Gram-negative pathogens. This study aimed to investigate the antibiotic susceptibility of 69 NTS isolates collected from the pork retail outlets and slaughterhouses in Vietnam during 2014 a nd 2018/19. Phenotypic testing and whole genome sequencing was used to assess the serotype and AMR gene profiles of the 69 NTS isolates. Seventeen different serotypes were identified, of which S. enterica subsp enterica serotype Typhimurium was the most common followed by S. ser. Rissen, S. ser. London, S. ser. Anatum, and S. ser. Derby. Phenotype AMR was common with 41 (59.4%) isolates deemed MDR. MDR strains were most common in slaughterhouses (83%) and supermarkets (75%) and lowest in traditional markets (38%) and convenience stores (40%). Colistin resistance was identified in 18 strains (15 resistant, three intermediate) with mcr-1 identified in seven isolates (S. ser. Meleagridis, S. Rissen, S. Derby) and mcr-3 in two isolates (S. Typhimurium). This includes the first mcr positive S. Meleagridis to our knowledge. Surprisingly, boutique stores had high levels (60%) of MDR isolates including 5/20 isolates with mcr-1. This study demonstrates that pork from modern retail stores classed as supermarkets or boutique (with pork claiming to be high quality, traceable, environmentally friendly marketed toward higher income consumers) still contained NTS with high levels of AMR. 2022-03-29 2022-04-03T11:21:39Z 2022-04-03T11:21:39Z Journal Article https://hdl.handle.net/10568/119225 en Open Access Frontiers Media Holohan, N., Wallat, M., Thi Hai Yen Luu, Clark, E., Duong Thi Quy Truong, Sinh Dang-Xuan, Hue Thi Kim Vu, Dung Van Truong, Hoang Tran Huy, Hung Nguyen-Viet, Unger, F., Son Thi Thanh Dang and Stabler, R.A. 2022. Analysis of antimicrobial resistance in non-typhoidal Salmonella collected from pork retail outlets in Vietnam using whole genome sequencing. Frontiers in Veterinary Science 9: 816279. |
| spellingShingle | antimicrobial resistance animal products swine Holohan, N. Wallat, M. Thi Hai Yen Luu Clark, E. Duong Thi Quy Truong Sinh Dang-Xuan Hue Thi Kim Vu Dung Van Truong Hoang Tran Huy Hung Nguyen-Viet Unger, Fred Son Thi Thanh Dang Stabler, R.A. Analysis of antimicrobial resistance in non-typhoidal Salmonella collected from pork retail outlets in Vietnam using whole genome sequencing |
| title | Analysis of antimicrobial resistance in non-typhoidal Salmonella collected from pork retail outlets in Vietnam using whole genome sequencing |
| title_full | Analysis of antimicrobial resistance in non-typhoidal Salmonella collected from pork retail outlets in Vietnam using whole genome sequencing |
| title_fullStr | Analysis of antimicrobial resistance in non-typhoidal Salmonella collected from pork retail outlets in Vietnam using whole genome sequencing |
| title_full_unstemmed | Analysis of antimicrobial resistance in non-typhoidal Salmonella collected from pork retail outlets in Vietnam using whole genome sequencing |
| title_short | Analysis of antimicrobial resistance in non-typhoidal Salmonella collected from pork retail outlets in Vietnam using whole genome sequencing |
| title_sort | analysis of antimicrobial resistance in non typhoidal salmonella collected from pork retail outlets in vietnam using whole genome sequencing |
| topic | antimicrobial resistance animal products swine |
| url | https://hdl.handle.net/10568/119225 |
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