Prevalence, antibiogram, and multidrug-resistant profile of E. coli O157: H7 in retail raw beef in Addis Ababa, Ethiopia
Escherichia coli O157:H7 is an emerging foodborne pathogen of public health importance. The objectives of this study were to estimate the prevalence and evaluate the antimicrobial susceptibility pattern and multidrug-resistant profile of E. coli O157:H7 isolated from raw beef sold in butcher shops i...
| Autores principales: | , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
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Frontiers Media
2022
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| Acceso en línea: | https://hdl.handle.net/10568/118241 |
| _version_ | 1855539371086708736 |
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| author | Haile, A.F. Alonso, Silvia Belay, N. Atoma, T.B. Boyaka, P.N. Grace, Delia |
| author_browse | Alonso, Silvia Atoma, T.B. Belay, N. Boyaka, P.N. Grace, Delia Haile, A.F. |
| author_facet | Haile, A.F. Alonso, Silvia Belay, N. Atoma, T.B. Boyaka, P.N. Grace, Delia |
| author_sort | Haile, A.F. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Escherichia coli O157:H7 is an emerging foodborne pathogen of public health importance. The objectives of this study were to estimate the prevalence and evaluate the antimicrobial susceptibility pattern and multidrug-resistant profile of E. coli O157:H7 isolated from raw beef sold in butcher shops in Addis Ababa, Ethiopia. A total of 384 raw beef samples were collected from randomly selected butcher shops across the 10 sub-cities of Addis Ababa. E. coli O157:H7 was isolated following ISO-16654:2001 standard, and isolates were tested for resistance to 13 antimicrobial agents using the Kirby–Bauer disk diffusion method. Out of the 384 retail raw beef samples examined, 14 (3.64%) (95% CI = 1.77–5.51%) carried E. coli O157:H7 serotype. Of the 14 E. coli O157:H7 isolates, 8 (57.14%) were found to be resistant to three or more antimicrobial categories. The frequency of resistant phenotype was more common for ampicillin (92.8%), nitrofurantoin (92.8%), and tetracycline (50%). Multidrug-resistant E. coli O157:H7 were present in raw beef sold in butcher shops in Addis Ababa. Thus, more stringent monitoring of antimicrobial use in both human and animal populations should be implemented. In addition, further studies should be conducted to understand the E. coli O157:H7 points of contamination and define appropriate risk mitigation strategies. |
| format | Journal Article |
| id | CGSpace118241 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Frontiers Media |
| publisherStr | Frontiers Media |
| record_format | dspace |
| spelling | CGSpace1182412025-12-08T10:29:22Z Prevalence, antibiogram, and multidrug-resistant profile of E. coli O157: H7 in retail raw beef in Addis Ababa, Ethiopia Haile, A.F. Alonso, Silvia Belay, N. Atoma, T.B. Boyaka, P.N. Grace, Delia antimicrobial resistance food safety animal products meat Escherichia coli O157:H7 is an emerging foodborne pathogen of public health importance. The objectives of this study were to estimate the prevalence and evaluate the antimicrobial susceptibility pattern and multidrug-resistant profile of E. coli O157:H7 isolated from raw beef sold in butcher shops in Addis Ababa, Ethiopia. A total of 384 raw beef samples were collected from randomly selected butcher shops across the 10 sub-cities of Addis Ababa. E. coli O157:H7 was isolated following ISO-16654:2001 standard, and isolates were tested for resistance to 13 antimicrobial agents using the Kirby–Bauer disk diffusion method. Out of the 384 retail raw beef samples examined, 14 (3.64%) (95% CI = 1.77–5.51%) carried E. coli O157:H7 serotype. Of the 14 E. coli O157:H7 isolates, 8 (57.14%) were found to be resistant to three or more antimicrobial categories. The frequency of resistant phenotype was more common for ampicillin (92.8%), nitrofurantoin (92.8%), and tetracycline (50%). Multidrug-resistant E. coli O157:H7 were present in raw beef sold in butcher shops in Addis Ababa. Thus, more stringent monitoring of antimicrobial use in both human and animal populations should be implemented. In addition, further studies should be conducted to understand the E. coli O157:H7 points of contamination and define appropriate risk mitigation strategies. 2022-02-24 2022-02-24T10:46:12Z 2022-02-24T10:46:12Z Journal Article https://hdl.handle.net/10568/118241 en Open Access Frontiers Media Haile, A.F., Alonso, S., Belay, N., Atoma, T.B., Boyaka, P.N. and Grace, D. 2022. Prevalence, antibiogram, and multidrug-resistant profile of E. coli O157: H7 in retail raw beef in Addis Ababa, Ethiopia. Frontiers in Veterinary Science 9: 734896. |
| spellingShingle | antimicrobial resistance food safety animal products meat Haile, A.F. Alonso, Silvia Belay, N. Atoma, T.B. Boyaka, P.N. Grace, Delia Prevalence, antibiogram, and multidrug-resistant profile of E. coli O157: H7 in retail raw beef in Addis Ababa, Ethiopia |
| title | Prevalence, antibiogram, and multidrug-resistant profile of E. coli O157: H7 in retail raw beef in Addis Ababa, Ethiopia |
| title_full | Prevalence, antibiogram, and multidrug-resistant profile of E. coli O157: H7 in retail raw beef in Addis Ababa, Ethiopia |
| title_fullStr | Prevalence, antibiogram, and multidrug-resistant profile of E. coli O157: H7 in retail raw beef in Addis Ababa, Ethiopia |
| title_full_unstemmed | Prevalence, antibiogram, and multidrug-resistant profile of E. coli O157: H7 in retail raw beef in Addis Ababa, Ethiopia |
| title_short | Prevalence, antibiogram, and multidrug-resistant profile of E. coli O157: H7 in retail raw beef in Addis Ababa, Ethiopia |
| title_sort | prevalence antibiogram and multidrug resistant profile of e coli o157 h7 in retail raw beef in addis ababa ethiopia |
| topic | antimicrobial resistance food safety animal products meat |
| url | https://hdl.handle.net/10568/118241 |
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